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Anti dhpr

Manufactured by Thermo Fisher Scientific
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The Anti-DHPR is a laboratory reagent used for the detection and quantification of DHPR (dihydrolipoamide dehydrogenase) in biological samples. It functions as an antibody that specifically binds to the DHPR protein, enabling researchers to analyze the presence and levels of this enzyme in their experiments.

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Lab products found in correlation

Sc 51313, by Santa Cruz Biotechnology (1 mentions) Sc 23918, by Santa Cruz Biotechnology (1 mentions) Ma3 920, by Thermo Fisher Scientific (1 mentions) Anti α actin, by Abcam (1 mentions) Anti orai1, by Abcam (1 mentions) Anti gst, by Thermo Fisher Scientific (1 mentions) Anti serca1a, by Thermo Fisher Scientific (1 mentions) Anti jp1, by Thermo Fisher Scientific (1 mentions) Anti jp2, by Thermo Fisher Scientific (1 mentions) Anti ryr1, by Thermo Fisher Scientific (1 mentions) Anti stim1, by Abcam (1 mentions)

2 protocols using anti dhpr

1

Immunoblotting Analysis of Quadriceps Muscle Proteins

Cited 1 time
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Proteins in lysates of 2-3month old wildtype and Ehd1-heterozygous quadriceps muscles, (n ≥ 2), were transferred to membranes and were immunoblotted with anti-BIN1 (1:1000, sc-23918, Santa Cruz, Dallas, TX), rabbit polyclonal anti-EHD1 (1:5000, [10 (link)]), anti-DHPR (1:1000, ma3-920, Thermo Scientific, Rockford, IL), anti-Junctophilin 2 (1:1000, sc-51313, Santa Cruz, Dallas, TX), anti-Junctophilin 1 (1:1000, 40–5100, Thermo Scientific, Rockford, IL) antibodies. Secondary antibodies, goat anti-rabbit, and goat anti-mouse conjugated to horseradish peroxidase (Jackson ImmunoResearch) were used at a dilution of 1:2500. Blocking and antibody incubations were done in Starting Block T20 (Invitrogen, Grand Island, NY) for all antibodies and rinsed with TBS-T. Memcode and gelcode stains were used to mark proteins for loading controls (Life Technologies, Grand Island, NY). Chemiluminescence substrate, Kodak Biomax MS film, and a UVP BioSpectrum Imaging System (Upland, CA) were used for detection.
Demonbreun A.R., Swanson K.E., Rossi A.E., Deveaux H.K., Earley J.U., Allen M.V., Arya P., Bhattacharyya S., Band H., Pytel P, & McNally E.M. (2015). Eps 15 Homology Domain (EHD)-1 Remodels Transverse Tubules in Skeletal Muscle. PLoS ONE, 10(9), e0136679.
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2

Triad Vesicles Containing TRPC3

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The triad vesicles containing TRPC3 were prepared from rabbit fast-twitch skeletal muscle and were solubilized, as previously described [4 (link),6 (link),25 (link),29 (link)]. All surgical interventions and methods of animal care were conducted under the guidelines, as previously described [6 (link),27 (link),30 (link)]. The solubilized triad sample or the lysate of myotubes was subjected to co-immunoprecipitation assay using anti-TRPC3 antibody [28 (link),30 (link)]. For immunoblot assays, various antibodies were used: anti-RyR1, anti-DHPR, anti-SERCA1a, anti-MG29, anti-JP1, anti-JP2, and anti-GST antibodies (1:1,000) from Thermo Scientific Inc. (Rockford, IL, USA), anti-TRPC3 and anti-TRPC4 antibodies (1:800) from Alomone Laboratories (Jerusalem 9104201, Israel), and anti-Orai1, anti-STIM1, and anti-α-actin antibodies (1:1,000) from Abcam (Cambridge, MA, USA).
Woo J.S., Hwang J.H., Huang M., Ahn M.K., Cho C.H., Ma J, & Lee E.H. (2015). Interaction between mitsugumin 29 and TRPC3 participates in regulating Ca2+ transients in skeletal muscle. Biochemical and biophysical research communications, 464(1), 133-139.
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