Slc32a1 cop4 h134r eyfp

Adult mice were employed (>P60, 20–24 g), and housed in single cages on a normal light-dark cycle. For the Cre-dependent TeLC group, offspring of Emx1^Cre/Cre breeding pairs were obtained from The Jackson Laboratory (005628). For the optogenetic experiments, the mice employed were: adult Thy1^{ChR2/ChR2-YFP}, line 18 (Thy1-COP4/EYFP, Jackson Laboratory, 007612) and VGAT ^ChR2/+, line 8 (Slc32a1-COP4*H134R/EYFP, Jackson Laboratory, 014548). For the combined optogenetics and TeLC expression experiments, adult offspring of Emx1^Cre/Cre mice crossed with Thy1^{ChR2/ChR2-YFP} mice were employed.

All experiments were carried out in accordance with the guidelines given by Landesamt für Gesundheit und Soziales (LAGeSo - G 0142/18) Berlin and were approved by the authority. Adult VGAT-ChR2 (Slc32a1-COP4∗H134R/EYFP, The Jackson Laboratory, stock no. 014548, n=9) transgenic mice of either sex were used to activate INs (Zhao et al., 2011 (link)). Mice were aged 7–9 months on the day of recordings.

Animal procedures were performed in accordance with protocols (Protocol number: 13–99) approved by the Institutional Animal Care and Use Committee (IACUC) of the Janelia Research Campus. Animals were housed on a 12-hr light/dark schedule with ad libitum water. Mice undergoing behavioral training were food restricted to 80–90% of original body weight by limiting food intake to 2–3 g/day. Otherwise, mice had ad libitum food. Animals were monitored daily by veterinary staff, and animals recovering from surgery or on food restriction were given a Pain Assessment Score of 1–5, based on IACUC guidelines. Animals with a Pain Assessment Score above 3 were temporarily removed from behavioral testing, given analgesia as determined by the veterinarian, and in some cases their food allotment was increased. Animals were returned to food restriction and behavioral testing after three consecutive days with a Pain Assessment Score below 3. Slc32a1-COP4*H134R/EYFP (Stock Number: 014548) were obtained from The Jackson Laboratory (Bar Harbor, ME).