A 10 mL blood sample was taken from the antecubital vein for an analysis of serum inflammatory cytokine (e.g., IL-1β, IL-6, and IL-8), BDNF, Aβ1-40, and Aβ1-42 levels. To permit clotting, the blood samples were incubated at room temperature (BD Vacutainer Plus), after which they were centrifuged at 2500 rpm at 4 °C for 15 min (Hettich Mikro 22R, C1110, Hettich, Tuttlingen, Germany). The serum was stored at −80 °C in small aliquots. Inflammatory cytokines and BDNF levels were analyzed using human cytokine antibody-immobilized magnetic beads (Millipore, Billerica, MA, USA), and a Luminex 200 analyzer (Luminex, Austin, TX, USA) was used to perform the measurements. The levels of Aβ1-40 and Aβ1-42 biomarkers were measured using single molecule counting (SMC®) immunoassay technology with commercially available kits obtained from Millipore and Sigma (Aβ1-40: # 03-0145-00, Aβ1-42: # 03-0146-00). A single individual performed the entire procedure used to determine the molecular markers in order to avoid interoperator bias. In terms of inter- and intra-assay precision, blood samples were run on multiple plates over a period of 3 days. Spiked and unspiked samples were within 20% across experiments.
Human cytokine antibody immobilized magnetic beads
Manufactured by Merck Group
Sourced in United States
Human cytokine antibody-immobilized magnetic beads are a type of laboratory equipment used in the detection and analysis of cytokines, which are signaling proteins involved in immune responses. These beads have antibodies specific to human cytokines immobilized on their surface, allowing for the capture and isolation of cytokines from biological samples. The magnetic properties of the beads enable easy separation and manipulation during the analysis process.
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Lab products found in correlation
2 protocols using human cytokine antibody immobilized magnetic beads
1
Biomarker Analysis of Blood Samples
2
Acute Exercise Effects on Serum Biomarkers
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A 10-mL blood sample were obtained from the antecubital vein via an aseptic technique at three time points (T1: before the 1st cognitive task test; T2: before the 2nd cognitive task about 5 min after acute exercise; and T3: immediately after the 2nd cognitive task test) by a qualified phlebotomist. The blood samples were withdrawn for analysis of serum BDNF, IGF-1, VEGF, and FGF-2 levels. Blood samples were obtained via an indwelling catheter located in a forearm vein during the T2 and T3 time points, with sterile saline to flush the catheter to prevent clot formation. The catheter was cleared of saline prior to each sample collection. The blood samples were kept at room temperature to allow for clotting (BD Vacutainer Plus), and then centrifuged at 3000 rpm for 15 min at 4 °C (Hettich Mikro 22R, C1110). Samples were harvested, aliquoted, and stored at − 80 °C for further serum marker assays. The levels of serum BDNF, IGF-I, VEGF and FGF-2 were analyzed by Human Cytokine Antibody-Immobilized Magnetic beads (Millipore, Billerica, MA, USA). Measurements were performed on a Luminex 200 analyzer (Luminex, Austin, TX, USA). The whole procedure for the determination of the four molecular markers was performed by the same person to avoid inter-operator bias.
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