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Human angiogenesis protein array

Manufactured by R&D Systems
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The Human Angiogenesis Protein Array is a laboratory tool designed to detect and measure the levels of various proteins involved in the process of angiogenesis, which is the formation of new blood vessels. The array allows for the simultaneous analysis of multiple angiogenesis-related proteins in a single experiment.

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Lab products found in correlation

Imagequant las 4000 biomolecular imager, by GE Healthcare (1 mentions)

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Human Angiogenesis Array

2 protocols using human angiogenesis protein array

1

Angiogenic Factors Secretion Assay

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Secretion of angiogenic factors was analyzed using a human angiogenesis protein array (R&D Systems, Minneapolis, USA). For this approach, supernatants of three unstimulated and three LPS-stimulated SDSC-seeded scaffolds (3 x 105 stem cells/6 mm-scaffold cultured in 1 ml medium) were collected 24 h after LPS treatment and analyzed following the manufacturer’s instructions (pooled 1:3). 10% FBS-DMEM was used as negative control.
Kisch T., Weber C., Rapoport D.H., Kruse C., Schumann S., Stang F.H., Siemers F, & Matthießen A.E. (2015). LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration. PLoS ONE, 10(11), e0142907.
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2

Quantitative Angiogenesis Protein Profiling

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The cell lysate was examined with the Human Angiogenesis Protein Array (ARY007, R&D Systems, Minneapolis, MN, USA), following the manufacturer’s protocol [31 (link)]. The results of the protein array were quantified with the ImageQuant™ LAS 4000 biomolecular imager (GE Healthcare Life Sciences, Chicago, IL, USA).
Lin L.W., Wang S.W., Huang W.C., Huynh T.K., Lai C.Y., Ko C.Y., Fong Y.C., Lee J.J., Yang S.F, & Tang C.H. (2023). Melatonin Inhibits VEGF-Induced Endothelial Progenitor Cell Angiogenesis in Neovascular Age-Related Macular Degeneration. Cells, 12(5), 799.
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