EPS matrix (2 mg) or CW preparation of S. indica or B. sorokiniana were ground with a stainless steel bead (5 mm) using a TissueLyser mill at 30 Hz for 1 min. The powdered material was subjected to glycosyl linkage analysis as described (Liu et al., 2015 (link)). Briefly, a methylation reaction was performed using NaOH/DMSO. The methylated compounds were hydrolyzed in 1 M trifluoroacetic acid, reduced using sodium borodeuteride (ACROS Organics, cat.no. 194950050) and per-o-acetylated. The resulting partially methylated alditol acetates were analyzed using an Agilent 5977A GC/MSD System equipped with a SP-2380 Fused Silica Capillary Column (Supelco). The glycosidic linkages were assigned based on retention time and mass spectrum fragmentation patterns compared to the CCRC spectral database (https://www.ccrc.uga.edu/specdb/ms/pmaa/pframe.html ).
Agilent 5977a gc msd system
Manufactured by Agilent Technologies
The Agilent 5977A GC/MSD System is a gas chromatography-mass spectrometry (GC/MS) instrument designed for analytical laboratories. It combines a gas chromatograph with a mass selective detector, enabling the identification and quantification of chemical compounds in complex samples. The system provides high-performance separation and detection capabilities for a wide range of applications, including environmental analysis, food testing, and pharmaceutical research.
Automatically generated - may contain errors
3 protocols using agilent 5977a gc msd system
1
Glycosyl Linkage Analysis of Polysaccharides
2
Purity Characterization by GC-MS and NMR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purity was inspected by GC–MS (43 (link)) and NMR. Samples were dissolved in ethyl acetate and subjected to GC–MS on an Agilent 5977A GC/MSD system—7890B Agilent GC system with an ultrainert GC column (19091S-433UI) followed by an Agilent 5977A MS instrument. Helium was used as the carrier gas at a constant flow of 0.7 mL/min, and 1:50 split samples were injected. Injection port and MS source temperature were held constant at 230 °C, and the MS quad temperature was held constant at 150 °C. The column temperature gradient was as follows: 70 °C for 0.5 min, 25 °C/min to 150 °C, 15 °C/min to 200 °C, and 25 °C/min to 300 °C and held at the upper temperature for 1 min. Results were analyzed on Mass Hunter software, the mass spectrum was compared to Wiley/NIST 2014 library (SI Appendix, Fig. S3A–C ). For NMR analysis, samples were dissolved in deuterated chloroform (CDCl3) in NMR tubes and were analyzed using an AVANCE III-400 device (Bruker) in 1H, 13C, and DEPT NMR modes. Resulting spectra were compared to National Institute of Advanced Industrial Science and Technology (AIST) spectral database. (SI Appendix, Fig. S3 D and E )
3
Glycosyl Linkage Analysis of Polysaccharides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EPS matrix (2 mg) or CW preparation of S. indica or B. sorokiniana were ground with a stainless steel bead (5 mm) using a TissueLyser mill at 30 Hz for 1 minute.
The powdered material was subjected to glycosyl linkage analysis as described (Liu et al., 2015) . Briefly, a methylation reaction was performed using NaOH/DMSO. The methylated compounds were hydrolyzed in 1 M trifluoroacetic acid, reduced using sodium borodeuteride (ACROS Organics, cat.no. 194950050 ) and per-o-acetylated.
The resulting partially methylated alditol acetates were analyzed using an Agilent 5977A GC/MSD System equipped with a SP-2380 Fused Silica Capillary Column was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted May 10, 2021. ; https://doi.org/10.1101/2021.05.10.443455 doi: bioRxiv preprint (Supelco). The glycosidic linkages were assigned based on retention time and mass spectrum fragmentation patterns compared to the CCRC spectral database (https://www.ccrc.uga.edu/specdb/ms/pmaa/pframe.html).
The powdered material was subjected to glycosyl linkage analysis as described (Liu et al., 2015) . Briefly, a methylation reaction was performed using NaOH/DMSO. The methylated compounds were hydrolyzed in 1 M trifluoroacetic acid, reduced using sodium borodeuteride (ACROS Organics, cat.no. 194950050 ) and per-o-acetylated.
The resulting partially methylated alditol acetates were analyzed using an Agilent 5977A GC/MSD System equipped with a SP-2380 Fused Silica Capillary Column was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted May 10, 2021. ; https://doi.org/10.1101/2021.05.10.443455 doi: bioRxiv preprint (Supelco). The glycosidic linkages were assigned based on retention time and mass spectrum fragmentation patterns compared to the CCRC spectral database (https://www.ccrc.uga.edu/specdb/ms/pmaa/pframe.html).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!