The experiments were performed on male Spraque-Dawley rats (Charles River, Germany) weighing about 250–300 g. The animals were maintained under standard laboratory conditions of lighting (light phase: 7:00–19:00) and temperature (19–21 °C). The rats were age-matched and were housed five to a cage with free access to water and food. All manipulations were performed between 8:00–14.00. All procedures were conducted according to the guidelines of the National Institutes of Health Animal Care and Use Committee and were approved by the Ethics Committee of the Institute of Pharmacology, Polish Academy of Sciences in Krakow. Every effort was made to minimize animal suffering and to reduce the number of animals used.
Spraque dawley rats
Manufactured by Charles River Laboratories
Sourced in Germany
Sprague-Dawley rats are a common laboratory rat strain used in various research applications. They are known for their docile temperament and suitability for a wide range of studies. The Sprague-Dawley rat is a widely used animal model in the fields of toxicology, pharmacology, and biomedical research.
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Lab products found in correlation
Nih foxn1rnu immunocompromised nude rats, by Charles River Laboratories (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Glutamine, by Merck Group (1 mentions)
Cryostat, by Leica (1 mentions)
Phosphate buffered solution, by Thermo Fisher Scientific (1 mentions)
Urethane, by Merck Group (1 mentions)
5 protocols using spraque dawley rats
1
Sprague-Dawley Rat Housing and Experimental Protocols
2
Sprague-Dawley Rat Housing Protocol
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Adult male Spraque-Dawley rats (Charles River Laboratories, Sulzfeld, Germany), weighting 250–300 g are raised in Specific pathogen Free (SPF) grade animal laboratory. Animals are housed in groups of two under standard conditions at a temperature of 22°C ± 1 and a 12 h-12 h light/dark cycle starting at 7:00 AM with free access to food and water. All experiments are carried out according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals (publication no. 85–23, revised 1985) and approved by IACUC (Institutional Animal Care and Use Committee of Nanjing Medical University, Ethical no.14030134).
3
Animal Experiments for Tissue Research
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All experiments on animals or involving the use of animal tissues were performed in accordance with a project license held under the Animals (Scientific Procedures) Act 1986, Amendment Regulations 2012, following ethical review by the University of Cambridge animal welfare and ethical review body. Neonatal Spraque Dawley rats and NIH‐Foxn1rnu immunocompromised nude rats were obtained from Charles River Laboratories.
4
Isolation and Culture of Rat Cerebral Cortex Astrocytes
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Primary mixed glial cultures were derived from the cerebral cortices of P0‐P2 neonatal Spraque Dawley rats (Charles River Laboratories) and were matured along the previous guidelines (McCarthy & De Vellis, 1980 (link)), with minor modifications (Syed et al., 2008 (link)). Mixed glia cells were maintained for 10 days in culture after which flasks were shaken for 1 h at 260 rpm on an orbital shaker to remove the loosely attached microglia, and then overnight at 260 rpm to dislodge oligodendrocyte precursors. Astrocyte cultures were maintained in glial culture medium (high‐glucose DMEM [Sigma‐Aldrich] supplemented with 10% FBS, glutamine [Sigma‐Aldrich] and 1% Penicillin/Streptomycin) for at least 2 weeks before passaging and platting for calcium imaging and multi‐electrode array (MEA) recording.
5
Immunohistochemical Analysis of Medulla Oblongata
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Spraque‐Dawley rats (n = 3, Charles Rivers, Wilmington, MA) were anesthetized with intraperitoneal injection of urethane (Sigma, St. Louis, MO) and transcardially perfused using oxygenated Dulbecco's Modified Eagle's Medium/Ham F12 Medium (Sigma), followed by fixation in 4% formaldehyde (Fisher Scientific, Waltham, MA). The medulla oblongata was excised and postfixed for 24 h (4% formaldehyde). The tissue was then placed into 20% sucrose solution for 2 days or until the tissue sank to the bottom of the vial and was sectioned at 30 μm using a cryostat (Leica Microsystems, Buffalo Grove, IL). The transverse sections were transferred to 0.1 mol/L phosphate‐buffered solution (Fisher) for immunohistochemical staining.
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