The cell lines used in this study included human embryonic kidney cells (HEK293) and tumorigenic cells (cervix HeLa and lung H1299; American Tissue Type Collection, Manassas, VA, USA). Cells were cultured as described before (55 (link)). Cisplatin, vincristine and taxol (paclitaxel) were purchased from Bristol-Myers Squibb (New York, NY, USA). PFTα and histone acetyltransferase inhibitor (HATi II) were obtained from Biomol Research Laboratories (Farmingdale, NY, USA) and Calbiochem (San Diego, CA, USA), respectively. Other chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA). All reagents were used according to the instructions provided by the supplier.
Paclitaxel taxol
Manufactured by Bristol-Myers Squibb
Sourced in United States
Paclitaxel (Taxol) is a chemotherapeutic agent used in the treatment of certain types of cancer. It works by inhibiting cell division, thereby preventing the growth and spread of cancer cells. Paclitaxel is a naturally derived compound extracted from the bark of the Pacific yew tree.
Automatically generated - may contain errors
Lab products found in correlation
Naringenin, by Merck Group (2 mentions)
Naringin, by Merck Group (2 mentions)
Cisplatin, by Bristol-Myers Squibb (1 mentions)
Vincristine, by Bristol-Myers Squibb (1 mentions)
Verso 1 step rt pcr reddymix kit, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Uric acid, by Spinreact (1 mentions)
1 1 3 3 tetramethoxypropane, by Merck Group (1 mentions)
5 5 dithio bis nitrobenzoic acid, by Merck Group (1 mentions)
Ck mb, by Spinreact (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Bodipy tr cadaverine, by Thermo Fisher Scientific (1 mentions)
6 protocols using paclitaxel taxol
1
Anticancer Drug Treatment in Cell Lines
2
Taxol Hepatoprotective Assessment Protocol
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Taxol (paclitaxel) of batch number: 7E05628 was purchased from Bristol-Myers Squibb global biopharmaceutical company. Sigma (MO, USA) provided naringenin and naringin (batch codes: BCBM4171V and BCBJ2179V, respectively). Alkaline phosphatase (ALP) reagent kits, alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyltransferase (GGT), and GGT were all bought from Biosystem S.A. (Spain) with catalogue numbers of M11533c-21, M11531c-21, M11584c-11, and M11592-0610, respectively. Lactate dehydrogenase (LDH) reagent kit (catalogue number: MX41214) acquired from Spin React (Spain). Albumin and total bilirubin kits (catalogue numbers 10560 and 10,742 respectively) were attained from HUMAN Gesellschaft für Biochemica und Diagnostica mbH, Wiesbaden, Germany. Verso 1-Step RT-PCR Reddy Mix Kit from Thermo Scientific (Applied Biosystems, Foster City, CA, USA) (catalogue number: AB-1454-LD). All other chemicals were purchased commercially and were of analytical quality.
3
Ovarian Cancer Cell Lines and Drug Treatments
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Two human ovarian cancer cell lines were selected for this study: SKOV3ip1, which expresses high levels of vascular endothelial growth factor (VEGF) and is associated with increased ascites formation, and HeyA8, which is characterized by low VEGF expression and no ascites formation. Those cell lines are kind gifts of Dr Isaiah J. Fidler (The University of Texas MD Anderson Cancer Center, Houston, TX) [20] (link). Cells were maintained in monolayer cultures in Eagle's minimal essential medium supplemented with 10% FBS (Life Technologies, Inc, Grand Island, NY), l -glutamine, pyruvate, non-essential amino acids, two-fold vitamins, and penicillin-streptomycin (Invitrogen, Carlsbad, CA) and incubated at 37°C in 5% CO2 and 95% air.
The rhLK8 protein was expressed and purified to homogeneity as previously described [21] (link). Purified rhLK8 proteins were stored in buffer containing 100 mM NaCl and 150 mMl -glycine (pH 4.2). Paclitaxel (Taxol), purchased from Bristol-Myers Squibb (Princeton, NJ), was diluted in saline for intraperitoneal (i.p.) injection.
The rhLK8 protein was expressed and purified to homogeneity as previously described [21] (link). Purified rhLK8 proteins were stored in buffer containing 100 mM NaCl and 150 mM
4
Paclitaxel and Antioxidant Compound Evaluation
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Paclitaxel (Taxol®) was obtained from the Bristol–Myers Squibb global biopharmaceutical company (batch code: 7E05628). Naringin (batch code: BCBM4171 V) and naringenin (batch code: BCBJ2179 V) were obtained from Sigma (MO, USA). The creatinine reagent kit (catalog numbers: M11502c-18) and the urea reagent kit (catalog numbers: M11536c-16) were purchased from Biosystem S.A. (Spain). Uric acid, CK-MB, and LDH reagent kits were purchased from Spin React (Spain), with catalog numbers MD41001, MD41254, and MX41214, respectively. Chemicals of oxidative stress including trichloroacetic acid were obtained from PanReac AppliChem ITW Companies (Spain) (batch code: 5O011689), thiobarbitUric acid was obtained from Sd Fine Chem Limited(SDFCL) Company (India) (batch code: L 16 A/1916/1212/13), 1,1,3,3 tetra-methoxy propane was obtained from Sigma-Aldrich (MO, USA) (catalog no: T9889), metaphosphoric acid was obtained from ALPHA CHEMIKA Company (India) (batch code: M 21519), 5,5- dithiobis nitrobenzoic acid was obtained from Sigma-Aldrich (MO, USA) (batch code: 40K3652), GSH was obtained from PanReac AppliChem ITW Companies (Spain) (batch code: 3W010085), and pyrogallol was obtained from Research Lab Company (India) (batch code: 1280B251114).
5
Colorectal Cancer Sensitivity and Resistance
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HCT-8 and HCT-8/VCR, human colorectal cancer sensitive and multidrug resistant cell lines, respectively, were purchased from Gu Haibo Biological Technology Co., Ltd. (Shanghai, China). SalB was obtained from Shanghai Winherb Medical Technology Co., Ltd. (Shanghai, China). Vincristine (VCR) was manufactured by Shenzhen Main Luck Pharmaceuticals Inc. (Shenzhen, China). 5-Fluorouracil (5-FU), cisplatin (CDDP) and N-acetylcysteine (NAC) were purchased from Sigma-Aldrich (Merck Millipore, Darmstadt, Germany). Paclitaxel (Taxol) was from Bristol-Myers Squibb (New York, NY, USA). H2O2 was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Cell Counting Kit-8 (CCK-8) was manufactured by Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). Bicinchoninic acid (BCA) assay and ROS kits were from Beyotime Institute of Biotechnology (Haimen, China). A rabbit monoclonal antibody against P-gp (catalog no. ab170904) was purchased from Abcam (Cambridge, MA, USA). Rabbit monoclonal antibodies against Bcl-2 (catalog no. 4223), Bax (catalog no. 5023) and GAPDH (catalog no. 2118), and the horseradish peroxidase-conjugated secondary antibody mouse anti-rabbit IgG (catalog no. 5127), were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).
6
Bioconjugate Delivery of Paclitaxel and SN-38
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The hyaluronan-based paclitaxel (ONCOFID-P) [21] (link) and SN-38 (ONCOFID-S) [11] (link) bioconjugates have been previously described. The batch of ONCOFID-S used was characterized by a SN-38 loading of 9.4%. Paclitaxel (Taxol) was from Bristol-Myers Squibb Italia (Rome, Italy), while irinotecan (CPT-11) and SN-38 were purchased from Antibioticos (Rodano, Italy). When needed, bioconjugates were labeled with the fluorochrome BODIPY TR cadaverine (Invitrogen, San Giuliano Milanese, Italy), as previously described [33] (link).
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