Cd31 clone wm59

Manufactured by BioLegend

CD31 (clone WM59) is a cell surface protein that is expressed on endothelial cells, platelets, and certain types of leukocytes. It is involved in cell-cell adhesion and signaling processes. This antibody can be used for the identification and enumeration of CD31-positive cells in various research applications.

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Clone WM59 (3 citations) CD31-APC clone WM59 (2 citations)

3 protocols using cd31 clone wm59

Hematopoietic Cell Culture Protocols

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M199 and StemPro®-34 SFM culture media were purchased from Life Technologies (Carlsbad, CA). HPGM™ and StemSpan™ SFEM cell culture media were purchased from Lonza (Walkersville, MD) and STEMCELL Technologies (Vancouver, Canada), respectively. Human SCF, IL-6, and IL-3 were purchased from ProSpec (Rehovot, Israel) or PeproTech (Rocky Hill, NJ). Defined HyClone fetal bovine serum (FBS) was purchased from GE Healthcare Life Sciences (Logan, UT). Human fibroblasts treated with mitomycin C were purchased from Merck Millipore (Billerica, MA), while human umbilical vein ECs were purchased from PromoCell (Heidelberg, Germany). Anti-human fluorochrome-conjugated CD117/c-kit (clone 104D2), FcεRI (clone CRA-1), and CD31 (clone WM59), and their isotype controls, Ms IgG1 (clone MOPC-21) and Ms IgG2b (clone MPC-11), were purchased from BioLegend (San Diego, CA). Anti-human fluorochrome-conjugated CD90 (clone 5E10), its isotype, Ms IgG1 (clone MOPC-21), and mouse anti-human tryptase (clone AA1) were purchased from Abcam (Cambridge, MA). Mouse anti-human chymase (clone B7) and the secondary antibody goat anti-mouse IgG1 were from Chemicon International Inc. (Temecula, CA) and Santa Cruz Biotechnology (Dallas, TX), respectively.

Derakhshan T., Bhowmick R., Ritchey J.W, & Gappa-Fahlenkamp H. (2018). Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation. Stem Cells International, 2018, 2136193.

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Multiparametric Flow Cytometry Panel for Murine and Human Samples

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Antibodies for FACS of murine samples included CD31 (MEC13.3, BD Bioscience), CD45 (clone 30-F11, BD Bioscience), Ter119 (clone TER-119, BD Bioscience), CD249/BP-1 (clone 6C3, eBioscience), THY1.2 (clone 53-2.1, BD Bioscience), CD200 (clone OX-90, BD Bioscience), CD105 (clone MJ7/18, BioLegend), EMB (clone REA501, Miltenyi Biotec). Antibodies for FACS of human samples included CD31 (clone WM59, BioLegend), CD45 (clone HI30, BioLegend), CD235a (clone GA-R2, BioLegend), THY1-1 (clone 5E10, BD Bioscience), CD200 (clone MRC OX-104, BD Bioscience), CD105 (clone 43A3, BioLegend), EMB (Rabbit monoclonal [EPR11417], Abcam), CD146 (clone P1H12, BD Bioscience), Podoplanin (clone LpMab-17, BD Bioscience), CD73 (clone AD2, BD Bioscience), CD164 (clone N6B6, BD Bioscience), HLA-ABC (clone W6/32, BioLegend), B2M(beta2-microglobulin) (Clone 2M2, BioLegend), Goat anti-Rabbit IgG Alexa Fluor Plus 647 (polyclonal, A32733, Invitrogen) and Donkey anti-Rabbit IgG Alexa Fluor Plus 555(polyclonal,A32794, Invitrogen).

Sun J., Hu L., Bok S., Yallowitz A.R., Cung M., McCormick J., Zheng L.J., Debnath S., Niu Y., Tan A.Y., Lalani S., Morse K.W., Shinn D., Pajak A., Li Z., Li N., Xu R., Iyer S, & Greenblatt M.B. (2023). Discovery of a Vertebral Skeletal Stem Cell Driving Spinal Metastases. Research Square.

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Immunofluorescent Analysis of Human GBM Tumors

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Human GBM tumors were sliced into thin sections manually with a sharp surgical blade. Sections were incubated overnight at 4 degrees C with 2 μg/mL PE-conjugated HLA-A2 tetramer in PBS supplemented with 2% FBS and DNAse. Sections were then fixed in 2% paraformaldehyde for 2 h before being treated with 30% sucrose overnight for cryoprotection. Tissues were then frozen and sectioned as described above. PE-tetramer was amplified with a polyclonal rabbit-anti-PE antibody at a 1:600 dilution (Novus Biologicals, NB120–7011) followed by goat anti-rabbit Cy3 at a 1:800 dilution (Jackson ImmunoResearch, 111–165-003). Sections were also co-stained with antibodies to human CD8a (clone RPA-T8, eBiosciences) and CD31 (clone WM59, Biolegend) and counterstained with DAPI to detect nuclei.

Ning J., Gavil N.V., Wu S., Wijeyesinghe S., Weyu E., Ma J., Li M., Grigore F.N., Dhawan S., Skorput A.G., Musial S.C., Chen C.C., Masopust D, & Rosato P.C. (2022). Functional virus-specific memory T cells survey glioblastoma. Cancer immunology, immunotherapy : CII, 71(8), 1863-1875.

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