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2 protocols using oxymorphone d3

1

Quantification of Opioids and Cannabinoids in Wastewater

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Morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone, Δ9-tetrahydrocannabinol (THC) and its metabolites 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) and THCCOOH-glucuronide were purchased from Cerilliant (Round Rock, TX, USA). The deuterated analogs THC-d3, THCCOOH-d3, THCCOOH-glucuronide-d3, Morphine-d3, oxycodone-d6, hydrocodone-d6, oxymorphone-d3 and hydromorphone-d6 were also purchased from Cerilliant. Strata XC 33 µm polymeric strong cation exchange cartridges of 3 mL/60 mg for calibrators and 6 mL/200 mg for quality control (QC) and authentic wastewater samples were purchased from Phenomenex (Torrance, CA, USA). Ultra-high performance liquid chromatography (UHPLC) grade methanol, dichloromethane and ammonium hydroxide were purchased from Pharmco-Aaper (Brookfield, CT). Isopropanol, liquid chromatography mass spectrometry grade acetonitrile, and Whatman glass microfiber filters (outside diameter 4.7 cm, particle retention 1.6 μm, and thickness 0.26 mm) were from Thermo Fisher Scientific (Waltham, MA, USA).
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2

Certified Reference Standards for Drugs

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Certified reference standards of 6-monoacetylmorphine, alprazolam, amphetamine, buprenorphine, benzoylecgonine, carisoprodol, chlordiazepoxide, clonazepam, cocaine, codeine, cotinine, dihydrocodeine, diazepam, EDDP, fentanyl, flurazepam, hydrocodone, hydromorphone, lorazepam, MDA, MDEA, MDMA, methamphetamine, meperidine, morphine, methylphenidate, methadone, naloxone, norbuprenorphine, nicotine, nordiazepam, norfentanyl, noroxycodone, alpha-hydroxyalprazolam, oxazepam, oxycodone, oxymorphone, tapentadol, temazepam, ll-nor-9-Carboxy-Δ9-THC, and cis-tramadol were purchased, each at a concentration of 1 mg/mL, from Cerilliant Corporation (Round Rock, TX). Isotopically labeled internal standards (IS) morphine-D3, codeine- D6, hydrocodone-D3, 6-monoacetylmorphine-D6, hydromorphone-D6, oxymorphone-D3, noroxycodone-D3, and naloxone-D5 were purchased, each at a concentration of 100 μg/mL, from Cerilliant Corporation (Round Rock, TX). Drug-free urine was obtained from Bio-Rad Laboratories (Irvine, CA). High-performance liquid chromatography (HPLC) grade water, methanol, and formic acid were purchased from Fisher Scientific (Pittsburgh, PA) and ammonium formate was purchased from Sigma Aldrich (St. Louis, MO).
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