Dissection microscope

Manufactured by Leica

Sourced in Germany, United States

The Leica Dissection Microscope is a precision optical instrument designed for detailed examination and manipulation of small objects. It provides a clear, magnified view of specimens, enabling detailed observation and analysis.

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Lab products found in correlation

Isoflurane, by Zoetis (3 mentions) Maxq 4000, by Thermo Fisher Scientific (2 mentions) Trypan blue, by Merck Group (2 mentions) Whatman, by Cytiva (2 mentions) Isoflurane, by Leica (2 mentions) Meloxacam, by Boehringer Ingelheim (2 mentions) Metacam, by Boehringer Ingelheim (2 mentions) Matrigel, by BD (1 mentions) Dpbs matrigel, by BD (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Ms 222, by Merck Group (1 mentions) Lithium heparin coated tubes, by BD (1 mentions) Coolpix 4500, by Nikon (1 mentions) Hawp04700, by Merck Group (1 mentions) Ap1004700, by Merck Group (1 mentions) C57bl 6 male mice, by Jackson ImmunoResearch (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MZ-FLIII Leica dissection microscope (11 citations) M205C dissection microscope (6 citations) Fluorescent dissection microscope (5 citations) MZ16A dissection microscope (3 citations) MZFLIII epiflourescence dissection microscope (3 citations) Laser dissection microscope (2 citations) Stereotaxic dissection microscope (2 citations) DMS300 Dissection Microscope (2 citations) Leica 9Si dissection microscope (2 citations) Stereo dissection microscope (2 citations)

47 protocols using dissection microscope

Basal Cell Transplantation for Mammary Gland Regeneration

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Basal cells (Lin^–CD24⁺CD29^high) were FACS sorted from 6-week-old female control and K14-rtTA;teto-Cre;Bmpr1a^fl/fl mice and resuspended in a 1:1 DPBS/Matrigel (BD) solution at a concentration of 2,000 cells per 10 μL. Cells and Matrigel solutions were injected in 10 μL into 3-week-old female NOD-SCID mice which cleared fat pads of the inguinal mammary glands. After transplantation, all mice received water with 2 mg/mL Dox and 10 mg/mL sucrose immediately. Eight weeks after transplantation, mouse inguinal mammary glands were analyzed. Ductal outgrowths were detected under dissection microscope (Leica, Wetzlar, Germany).

Shao C., Lou P., Liu R., Bi X., Li G., Yang X., Sheng X., Xu J., Lv C, & Yu Z. (2021). Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland. Frontiers in Cell and Developmental Biology, 9, 691050.

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Isolation of Mouse Embryonic Fibroblasts

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Mouse embryonic fibroblasts were obtained from E14.5 embryos of R26‐M2rtTA knock‐in mice. Using a dissection microscope (Leica), heads, limbs, vertebral columns, and internal organs were removed to make certain no multipotent cells were present in cultures. After dissection, 2–3 embryos were pooled, and tissue was dissociated in 0.15% Trypsin (Gibco) for 10–15 min to obtain single‐cell suspensions. Cells were plated in a single T75 tissue culture flask per embryo in MEF medium at 37°C and 5% CO₂ (Dulbecco's Modified Eagle Medium (Gibco #61965) supplemented with 10% FBS (Pan Biotech or Gibco), 1% Sodium Pyruvate (Gibco), 1% HEPES (Gibco) and 1% Penicillin/Streptomycin). Cells were split once at a 1:3 ratio when confluent before freezing. After thawing, cells were grown in T75 culture flasks until confluent before nucleofection.

Hersbach B.A., Fischer D.S., Masserdotti G., D.e.e.k.s.h.a., Mojžišová K., Waltzhöni T., Rodriguez‐Terrones D., Heinig M., Theis F.J., Götz M, & Stricker S.H. (2022). Probing cell identity hierarchies by fate titration and collision during direct reprogramming. Molecular Systems Biology, 18(9), e11129.

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Isolation of Glial Cells from Neonatal Mouse Brains

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Glial cultures were prepared from the brains of 4-10 C57BL/6 neonatal mice per experiment using the differential attachment method as described previously (3 (link), 53 (link)). In brief, P1–2 mouse pups were decapitated with scissors, brains dissected and meninges removed under a dissection microscope (Leica). Brain tissue was disassociated in Accutase at 37°C for 45 min., passed through a 70μm filter, washed with Dulbecco’s Modified Eagle Medium (DMEM), seeded onto poly-d-lysine-coated T75-flasks, and grown to confluence (7–10 days) in a humidified incubator at 37°C and 5% CO₂. Microglia were isolated by shaking the T-flasks at 37°C for 1h at 177rpm in an orbital shaker (ThermoScientific Max Q 4000).

Soto-Diaz K., Vailati-Riboni M., Louie A.Y., McKim D.B., Gaskins H.R., Johnson R.W, & Steelman A.J. (2021). Treatment With the CSF1R Antagonist GW2580, Sensitizes Microglia to Reactive Oxygen Species. Frontiers in Immunology, 12, 734349.

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Optic Nerve Crush in Zebrafish

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Optic nerve crush (ONC) was performed as previously described [20 (link), 21 (link), 30 (link)]. Briefly, zebrafish were anesthetized in 0.02% buffered tricaine (MS-222, Sigma-Aldrich) and placed under a dissection microscope (Leica, Deerfield, IL). Using sterile forceps (Dumont No. 5, FST), the connective tissue around the left eye was removed. The eye was gently lifted out of its orbit to expose the optic nerve. Then, the nerve was crushed for ten seconds, at a distance of 500 μm from the optic nerve head. Care was taken not to damage the ophthalmic artery running parallel to the nerve. After surgery, fish were returned to separate tanks for recovery.

Bollaerts I., Van houcke J., Beckers A., Lemmens K., Vanhunsel S., De Groef L, & Moons L. (2019). Prior Exposure to Immunosuppressors Sensitizes Retinal Microglia and Accelerates Optic Nerve Regeneration in Zebrafish. Mediators of Inflammation, 2019, 6135795.

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Aorta Harvesting and Tissue Sampling

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Mice were euthanised by CO₂ asphyxiation at the completion of the experiment. The aorta was exposed under a dissection microscope (Leica). Blood was collected into lithium heparin coated tubes (BD Bioscience) from the right ventricle and centrifuged to collect plasma that was stored at −80 °C for future assessment. The aorta was carefully flushed with phosphate buffered saline (PBS) through the left ventricle and harvested free from fat and connective tissue. The whole dissected aorta was digitally photographed (Coolpix 4500, Nikon) on a graduated template44 (link). Then aortic tissues were snap frozen in Optimal Cutting Temperature compound and stored at −80 °C for future analysis. Mice that died prior to completion of the experimental period were subject to post-mortem and aortas were harvested for subsequent aortic diameter measurement. Rupture was confirmed by intra-thoracic, intra-abdominal or retro-peritoneal blood.

Yu H., Moran C.S., Trollope A.F., Woodward L., Kinobe R., Rush C.M, & Golledge J. (2016). Angiopoietin-2 attenuates angiotensin II-induced aortic aneurysm and atherosclerosis in apolipoprotein E-deficient mice. Scientific Reports, 6, 35190.

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Nitrocellulose Filter Cell Culturing

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In these assays, 1 × 10⁷ cells were plated and allowed to develop on 47mm nitrocellulose filters (Millipore, HAWP04700) over 24 h [74]. Each filter was placed on an absorbent 3M paper pad (Millipore, AP1004700) soaked in phosphate buffer of varying pH. Phosphate buffer was prepared using varying amounts of KH₂PO₄ and K₂HPO₄ to modulate pH without altering ionic strength. Subsequent developmental phenotypes were imaged using a dissection microscope (Leica) and a QICAM FAST 1394 camera (QImaging).

Sharma D., Otto G., Warren E.C., Beesley P., King J.S, & Williams R.S. (2019). Gamma secretase orthologs are required for lysosomal activity and autophagic degradation in Dictyostelium discoideum, independent of PSEN (presenilin) proteolytic function. Autophagy, 15(8), 1407-1418.

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Xenotransplantation of Mammary Cells

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Sorted cells were pelleted and resuspended in 50% Matrigel, PBS with 50% fetal bovine serum, and 0.04% Trypan Blue (Sigma). Female nude recipient mice at 3 weeks old were anesthetized and a small incision was made to reveal the fourth pair of mammary glands, then a 10 μl volume containing indicated cells was injected into each of the cleared fat pads. Reconstituted mammary glands were harvested and examined 6–8 weeks after surgery. Outgrowth degree of mammary glands was detected under a dissection microscope (Leica, Germany) after carmine staining. For limiting dilution analyses, the repopulating frequency was calculated by the Extreme Limiting Dilution Analysis Program.

Wang R., Huang F., Wei W., Zhou Y., Ye Z., Yu L., Hu J, & Cai C. (2021). Programmed Cell Death Ligand 1 Is Enriched in Mammary Stem Cells and Promotes Mammary Development and Regeneration. Frontiers in Cell and Developmental Biology, 9, 772669.

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In-situ Hybridization and Nuclei Staining

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In-situ hybridization experiments were performed as described previously [21 (link)]. Cell nuclei were stained with 1 μg/ml DAPI (4-6-diamidino-2-phenylindole) in PBST for 20 minutes followed by several washing steps in PBST. Embryos were analyzed under a Leica dissection microscope equipped with a Leica DC100 digital camera. Brightness, contrast and colour values were adjusted if necessary, using the image processing software Adobe Photoshop CS2 (Version 9.0.1 for Apple Macintosh).

Hogvall M., Schönauer A., Budd G.E., McGregor A.P., Posnien N, & Janssen R. (2014). Analysis of the Wnt gene repertoire in an onychophoran provides new insights into the evolution of segmentation. EvoDevo, 5, 14.

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Biomechanical and Imaging Assessment of Murine Femurs

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Male C57BL/6 mice were purchased from Jackson Laboratories (Bar Harbor, ME). Animals were housed under a standard light cycle and had free access to feed (standard diet) and water. Ten mice were euthanized at 16 weeks of age, and hind limbs (n=10 right and n=5 left) were harvested for biomechanical testing and/or 3D XRM imaging. Age-matched PRG4 knockout mice (Prg4^−/−, n=6) were generated and maintained on a C57BL/6genetic background, as previously described (Abubacker et al., 2019 (link)). Limbs were disarticulated at the hip, followed by transection of the ligaments and careful isolation of distal femurs from tibiae and menisci with the help of a dissection microscope (Leica). Femurs were preserved gently wrapped in Kimwipe soaked in phosphate-buffered saline (PBS, pH 7.4) until the time of assessment. All samples were mechanically tested no longer than 3 hr after dissection to prevent tissue degradation. Contralateral legs, not subjected to biomechanical testing were preserved in 10% neutral buffered formalin (NBF) solution for 24 hr and stored in 70% ethanol for 24hr to 48 hr before imaging.

Masson A.O., Besler B., Edwards W.B, & Krawetz R.J. (2022). High spatial resolution analysis using automated indentation mapping differentiates biomechanical properties of normal vs. degenerated articular cartilage in mice. eLife, 11, e74664.

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Immunolabeling and Imaging of Intestinal Tissue

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Isolated small and large intestinal tissues from mice were flushed with ice-cold PBS. Tissues were opened longitudinally and spread with the epithelial (luminal) side up on Whatman filter paper or left as tubes. If left as tubes the intestinal tissue was cut under a dissection microscope (Leica) into ~2 mm thick strips or rings containing 1-2 rows of intact crypt-villus axes to image a side view of the intestine. Mouse and human biopsy tissues were fixed with 4% PFA in PBS for 1-2 hours at 4°C, washed three times in PBS and then permeabilized for at least 3 hours in PBST followed by 1 hour in blocking solution (PBST with 2.5% NDS, 1% BSA, 2.5% gelatin). For immunolabeling, samples were incubated with primary antibodies in PBST for 24 hours at room temperature followed by 8-10 washes in PBST. Samples were incubated overnight at room temperature with secondary antibodies conjugated with Alexa-488, RRX, or Alexa-647 (1:200 Life Technologies) in blocking solution with DAPI (2 μg/ml, 1:500). Samples were washed at least 8 times with PBST containing DAPI (1:500) for 4 hours at room temperature prior to tissue clearing.

Niec R.E., Chu T., Schernthanner M., Gur-Cohen S., Hidalgo L., Pasolli H.A., Luckett K.A., Wang Z., Bhalla S.R., Cambuli F., Kataru R.P., Ganesh K., Mehrara B.J., Pe’er D, & Fuchs E. (2022). Lymphatics act as a signaling hub to regulate intestinal stem cell activity. Cell stem cell, 29(7), 1067-1082.e18.

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