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Caspase 9 9502

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Caspase-9 (9502) is a protein that plays a key role in the apoptosis, or programmed cell death, pathway. It acts as an initiator caspase, activating other downstream caspases to execute the cell death process. This product is a recombinant protein that can be used for research purposes in the study of apoptosis and related cellular mechanisms.

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Lab products found in correlation

Fitc annexin 5 apoptosis detection kit, by BD (2 mentions) Clarity max western ecl blotting substrate, by Bio-Rad (1 mentions) Anti lc3b antibody l7543, by Merck Group (1 mentions) Antibodies against gapdh 6c5 sc 32233, by Santa Cruz Biotechnology (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Sytox red dead cell stain, by Thermo Fisher Scientific (1 mentions) Sybr green master mix, by Vazyme (1 mentions) Solutol hs 15, by MedChemExpress (1 mentions) Hematoxylin and eosin staining kit, by Beyotime (1 mentions) Phospho ire1α ab48187, by Abcam (1 mentions) Chop 15204 1 ap, by Proteintech (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Hct116, by American Type Culture Collection (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sw480, by American Type Culture Collection (1 mentions) Cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions) Doxycycline, by Merck Group (1 mentions) β actin sc 47778, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Caspase-9 (660 citations) Anti-Caspase 9(#9502) (3 citations) Caspases (2 citations)

5 protocols using caspase 9 9502

1

Western Blot Analysis of Autophagy

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All reagents were purchased from Sigma-Aldrich unless otherwise indicated (Sigma-Aldrich, Madison, WI, USA). Antibodies against GAPDH (6C5) (sc-32233) were from Santa Cruz Biotechnology, Santa Cruz, CA, and the Anti-LC3B (L7543) antibody was purchased from Sigma-Aldrich, WI. Beclin-1 (D40C5) (3495), Caspase-3 (9662), and Caspase-9 (9502) antibodies were procured from Cell Signaling Technology, Danvers, MA. Clarity Max™ Western ECL blotting substrates (1705062) were purchased from Bio-Rad Laboratories, Segrate, Italy.
Dutta B., Huang J., To J, & Tam J.P. (2019). LIR Motif-Containing Hyperdisulfide β-Ginkgotide is Cytoprotective, Adaptogenic, and Scaffold-Ready. Molecules, 24(13), 2417.
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2

Anticancer Effects of MY11 Compound

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MY11 (purity 95%) was synthesized and provided by pharmaceutical chemistry laboratory, East China University of Science and Technology, China [23 (link)]. RPMI 1640 and DMEM was purchased from Hyclone (Logan, UT, USA). MEM, Penicillin/Streptomycin and Fetal Bovine Serum (FBS) were obtained from Gibco (Invitrogen Corporation, New York, USA). Antibodies for Cyclin D1 (A19038), PUMA (A3752), Bcl-2 (A19693), Bax (A19684), NF-κB p65 (A19653), Phospho-NF-κB p65-S536 (AP0124), β-Actin (AC004) and secondary antibodies were purchased from ABclonal (Wuhan, China). Caspase-9 (#9502) and p21 (#2947) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). FITC Annexin V Apoptosis Detection Kit were purchased from BD Biosciences Company. SYTOX Red dead cell stain was purchased from Invitrogen. SYBR green master mix was purchased from Vazyme. Solutol HS-15 was obtained from MedChemExpress. Hematoxylin and Eosin Staining Kit were purchased from Beyotime.
Ye Q., Jiang Z., Xie Y., Xu Y., Ye Y., Ma L, & Pei L. (2022). MY11 exerts antitumor effects through activation of the NF-κB/PUMA signaling pathway in breast cancer. Investigational New Drugs, 40(5), 922-933.
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3

Investigating Molecular Mechanisms of Endoplasmic Reticulum Stress

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Methylseleninic acid (MSeA,
purity >95%) and palmitic acid (PA) (P0500) were purchased from
Sigma-Aldrich
(St. Louis, MO). IRE1α inhibitor 4μ8C was purchased from
MCE (Shanghai, China). Primary antibodies specific for phospho-eIF2α
(3398), c-PARP (9546), Bip (3183), Bim (2933), and caspase-9 (9502)
were purchased from Cell Signaling Technology (Beverly, MA). Phospho-IRE1α
(ab48187) was purchased from Abcam (Cambridge, MA). CHOP (15204-1-AP)
was purchased from Protein Tech (Rosemont, IL). Secondary antibodies
specific for rabbit and mouse immunoglobulins were purchased from
MBL International Corporation (Woburn, MA).
Zhang H., Zheng Y., Han D., Lu J., Yin S., Hu H, & Zhao C. (2021). Combination of Palmitic Acid and Methylseleninic Acid Induces Mitochondria-Dependent Apoptosis via Attenuation of the IRE1α Arm and Enhancement of CHOP in Hepatoma. ACS Omega, 6(24), 15708-15715.
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4

Colorectal Carcinoma Cell Line Apoptosis Assay

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Colorectal carcinoma cell lines, HCT116 and SW480, were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). MTT (3-(4,5-Dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide) and dimethyl sulfoxide (DMSO) were bought from Sigma-Aldrich (St. Louis, MO, USA). Annexin V-FITC Apoptosis Detection Kit was purchased from BD Pharmingen, Inc, (San Jose, CA, USA). The culture medium, RPMI 1640 and Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin and streptomycin were purchased from Grand Island Biological Company (GIBCO, Grand Island, NY, USA). The antibodies were obtained from the following sources: Bax (#2772), caspase-9 (#9502), and (HSPA) HSP70 (#4872) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA); Bcl-2 (ab196495), caspase-3 (ab90437), β-actin (ab227387), and rabbit polyclonal secondary antibody goat anti-rabbit IgG H and L (HRP) (ab97100) were from Abcam (Cambridge, MA, USA). Hoechst 33342 was procured from Thermo Fisher Scientific (Waltham, MA, USA).
Yurasakpong L., Nantasenamat C., Nobsathian S., Chaithirayanon K, & Apisawetakan S. (2021). Betulinic Acid Modulates the Expression of HSPA and Activates Apoptosis in Two Cell Lines of Human Colorectal Cancer. Molecules, 26(21), 6377.
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5

Functional Analysis of FOXM1 in Multiple Myeloma

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HMCLs, designated H929 or APR1, were chosen for studies on inducible knockdown (KD) of FOXM1. HMCLs, CAG or XG1, were used for studies on constitutive overexpression (OE) of FOXM1. All 4 cell lines had secretion of IgA29 (link)–32 (link) and in vitro culture conditions (37 °C, 5% CO2) in common. Oncogene-activating chromosomal translocations took the form of t(4;14) in case of H929 cells33 (link), t(11;14) in XG1 cells30 (link) and t(14;16) in ARP1 and CAG cells33 (link), 34 . Gene expression spikes on microarrays corresponded to the translocation status: FGFR3 and WHSC1 (better known as MMSET) in case of H929 cells, CCND1 (cyclin D1) in XG1 cells and MAF (c-MAF) in ARP1 and CAG cells35 (link). The status of the tumor suppressor p53 was wild-type in case of H929 cells36 (link), mutated in XG1 cells37 (link) and null in ARP1 and CAG cells34 . Antibodies for Western blotting were purchased from Santa Cruz Biotechnology (FOXM1, catalog number sc-500; CDK6, sc-7961; β-actin, sc-47778) or Cell Signaling Technology (caspase-8, 4927; cleaved caspase-8, 9496; caspase-9, 9502; caspase-3, 9668; cleaved caspase-3, 9661; PARP, 9542). Doxycycline and thiostrepton (TS) were from Sigma.
Gu C., Yang Y., Sompallae R., Xu H., Tompkins V.S., Holman C., Hose D., Goldschmidt H., Tricot G., Zhan F, & Janz S. (2015). FOXM1 is a therapeutic target for high-risk multiple myeloma. Leukemia, 30(4), 873-882.
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