Mouse tnf α elisa kit

Manufactured by Solarbio

Sourced in China

The Mouse TNF-α ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of mouse tumor necrosis factor-alpha (TNF-α) levels in cell culture supernatants, serum, and plasma samples.

Automatically generated - may contain errors

Lab products found in correlation

Mouse il 6 elisa kit, by Solarbio (3 mentions) Mouse il 1β elisa kit, by Solarbio (3 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Mouse ifn γ elisa kit, by Solarbio (2 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (2 mentions) β actin, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg h l hrp conjugate, by Cell Signaling Technology (1 mentions) Goat anti mouse igg h l hrp conjugate, by Cell Signaling Technology (1 mentions) Mda content assay kit, by Solarbio (1 mentions) Pparα, by GeneTex (1 mentions) Pparγ, by Cell Signaling Technology (1 mentions) C ebpα, by Cell Signaling Technology (1 mentions) Mouse myeloperoxidase elisa kit, by Solarbio (1 mentions) Ripa buffer, by Solarbio (1 mentions) Raw264.7 cell line, by American Type Culture Collection (1 mentions) Fetal bovine serum for cell culture, by Sartorius (1 mentions) Rpmi 1640 cell culture medium, by Solarbio (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions) Ros assay kit, by Solarbio (1 mentions) Cck 8, by Solarbio (1 mentions)

6 protocols using mouse tnf α elisa kit

Exploring PPAR Regulation and Metabolic Processes

Check if the same lab product or an alternative is used in the 5 most similar protocols

BBR (purity > 95%, 10006427) was purchased from Cayman Chemical (Ann Arbor, MI, USA). GSK0660 (GSK) was bought from Santa Cruz Biotechnology (Dallas, TX, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin (PS), trypsin-EDTA, 3-(4,5-dimethylthPSiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and Lipofectamine™ 2000 transfection reagent were obtained from Thermo Fisher Scientific (Waltham, MA, USA).
Antibodies were obtained for protein detection: CEBPα (8178T, Cell Signaling, Danvers, MA, USA), PPARδ antibody (GTX113250, GeneTex, Irvine, CA, USA), PPARα (GTX101098, GeneTex), PPARγ (2435S, Cell Signaling), HO-1 antibody (GeneTex, GTX101147), β-Actin (AM4302, Thermo Fisher, Waltham, MA, USA), goat anti-rabbit IgG (H + L)-HRP conjugate (98164S, Cell Signaling), and goat anti-mouse IgG (H + L)-HRP conjugate (91196S, Cell Signaling).
Biochemical kits were obtained from Fisher Scientific (Waltham, MA, USA) and Beijing Solarbio Science and Technology (Beijing, China): Stanbio™ Triglyceride Liquid Reagent (2100430, Fisher Scientific), Stanbio™ Cholesterol Liquid Reagent (1010430, Fisher Scientific), Stanbio™ Glucose Liquid Reagent (1070125, Fisher Scientific), High-Density Lipoprotein Cholesterol (HDL) content assay kit (BC5320, Solarbio), Mouse TNFα ELISA kit (SEKM-0034, Solarbio), and MDA content assay kit (BC0025, Solarbio).

Shou J.W, & Shaw P.C. (2023). Berberine Reduces Lipid Accumulation in Obesity via Mediating Transcriptional Function of PPARδ. International Journal of Molecular Sciences, 24(14), 11600.

+ Open protocol

+ Expand

Cytokine Profiling in Mouse Liver Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Obtained liver tissues were cut into pieces and homogenated on ice with an ultrasonic probe in radioimmunoprecipitation (RIPA) buffer (Solarbio) with 1 mM protein inhibitor. Protein concentrations were measured using a BCA assay kit. Lysates were centrifugated at 10,000 × g for 5 min at 4°C according to the instructions, and the suspension was collected and stored in −80°C for further use. Before tests, a pre-experiment was conducted to optimize the concentration. ELISAs were conducted to measure the following cytokines according to the instructions (mouse TNF-α ELISA kit, mouse IFN-γ ELISA kit, mouse IL-1β ELISA kit, mouse IL-6 ELISA kit, mouse IL-12 ELISA kit, and mouse myeloperoxidase ELISA kit, Solarbio).

Zhao J., Li Y., Jia R., Wang J., Shi M, & Wang Y. (2021). Mesenchymal Stem Cells-Derived Exosomes as Dexamethasone Delivery Vehicles for Autoimmune Hepatitis Therapy. Frontiers in Bioengineering and Biotechnology, 9, 650376.

+ Open protocol

+ Expand

Cytokine Quantification in Mouse Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum obtained from mice in all 4 groups was placed in a refrigerator at 4°C for 12 h and then centrifuged at 2,800 × g for 10 min. The supernatant of serum was collected for the determination of tumor necrosis factor-α (TNF-α) and IL-1β by biotin-labeled doubleantibody sandwich ELISA. The specific procedure was carried out using ELISA kits (Mouse IL-1β ELISA kit and Mouse TNF-α ELISA kit, Solarbio).

Hu M., Wu X., Luo M., Wei H., Xu D, & Xu F. (2020). Lactobacillus rhamnosus FLRH93 protects against intestinal damage in mice induced by 5-fluorouracil. Journal of dairy science, 103(6).

+ Open protocol

+ Expand

Synthetic Peptide Protocols for Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

2-[(aminocarbonyl)amino]-5-(4-fluorophenyl)-3-thiophenecarboxamide (TPCA-1; CAS. no. 507475-17-4) was purchased from Abmole Bioscience Inc. (Houston, TX, USA). Alfa Chemicals (Pune, India) provided sodium borohydride, chloroauric acid trihydrate (HAuCl₄·3H₂O), silver nitrate (AgNO₃), and sodium citrate. Synthetic peptides, such as MCKYFIKIVSKSAKKPVGLIGC and MCKYFIKIVSKSAKK(FITC)PVGLIGC (FITC-labeled peptide), were obtained from DGpeptides Co., Ltd (Hangzhou, China). The purity of all the synthetic peptides was >99.0%. RAW 264.7 cell line was provided by ATCC (Manassas, VA, USA). Solarbio (Beijing, China) provided 4′,6-diamidino-2-phenylindole (DAPI), RPMI 1640 cell culture medium, ROS assay kit, and mouse TNF-α ELISA Kit. The mouse IL-6 ELISA Kit was purchased from Liankebio (Hangzhou, China). Fetal bovine serum (FBS) for cell culture was obtained from Biological Industries Ltd (Beit Haemek, Israel).

, & Zhao J. (2019). Hyaluronic Acid-Modified and TPCA-1-Loaded Gold Nanocages Alleviate Inflammation. Pharmaceutics, 11(3), 143.

+ Open protocol

+ Expand

Modulation of Interferon Signaling by DDX5 and METTL3

Check if the same lab product or an alternative is used in the 5 most similar protocols

The MEFs were transfected with siRNA using Lipofectamine RNAiMAX reagents to silence the targeted genes; otherwise, the MEFs were transfected with plasmids using Lipofectamine 3000 reagent to express target proteins. HEK293T cells were co-transfected with several plasmids using Lipofectamine 3000 reagent to detect protein interactions. Mouse macrophages were infected with DDX5 lentiviruses to express DDX5 in the cells, or they were infected with siRNA adenovirus to silence the targeted gene. For ELISA, the supernatant from DDX5/METTL3-silenced or DDX5/METTL3-expressing MEFs was collected at 0–24hpi with VSV (MOI = 10) or SeV (MOI = 5), and the supernatants were analyzed by the LumiKine Xpress mIFN-β 2.0 ELISA Kit (InvivoGen, Hong Kong, China), mouse IL-6 ELISA Kit, and mouse TNF-α ELISA Kit (Solarbio, Beijing, China).The m6A content in total RNA was detected and calculated with the m6A RNA Methylation Assay Kit (Colorimetric) (Abcam, ab185912).

Xu J., Cai Y., Ma Z., Jiang B., Liu W., Cheng J., Guo N., Wang Z., Sealy J.E., Song C., Wang X, & Li Y. (2021). The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NFκB transcripts to dampen antiviral innate immunity. PLoS Pathogens, 17(4), e1009530.

+ Open protocol

+ Expand

Lactoferrin Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human recombinant lactoferrin (holo-LF) expressed in rice was purchased from Sigma (L1294-1G, the purity ≥90%). Using holo-LF as raw material, apo-LF was prepared according to the patent "a method for preparing lactoferrin with required iron saturation" (Luo et al., 2020) . By adding citrate buffer (0.5 M) with a pH of 2 to the holo-LF solution (0.1 M), the molar volume ratio of citrate buffer to holo-LF was 3:1, then adjusting the pH value of the solution to 5; next, the LF solution could be made into a sample with an iron saturation of 4.1%, which was regarded as the apo-type LF in the present study (Luo et al., 2020) . Lipopolysaccharide (L8880-10 mg, purity ≥99%) and inflammatory cytokine kits (mouse IL-1β ELISA kit, mouse IL-6 ELISA kit, mouse TNF-α ELISA kit, and mouse IFNγ ELISA kit) were purchased from Beijing Solarbio Technology Co. Ltd., and a cell counting kit-8 (CCK-8) was purchased from Solarbio. Hematoxylineosin (HE) and 3,3′-diaminobenzidine (DAB) staining kits were from Wuhan Seville Biotechnology Co. Ltd.

Fan L.L., Yao Q.Q., Wu H.M., Wen F., Wang J.Q., Li H.Y, & Zheng N. (2022). Protective effects of recombinant lactoferrin with different iron saturations on enteritis injury in young mice. Journal of dairy science, 105(6).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!