Ajax finechem

Manufactured by Thermo Fisher Scientific

Sourced in Australia, United Kingdom

The Ajax Finechem is a laboratory equipment designed for chemical analysis and synthesis. It provides precise temperature control and stirring functionality to support a variety of laboratory procedures. The core function of the Ajax Finechem is to enable controlled heating and mixing of chemical samples.

Automatically generated - may contain errors

Lab products found in correlation

Hepes, by Thermo Fisher Scientific (2 mentions) Low glucose dmem, by Thermo Fisher Scientific (2 mentions) Tp1020, by Leica (1 mentions) Polysine microscope slide, by Thermo Fisher Scientific (1 mentions) Rm2235, by Leica (1 mentions) Histocore arcadia h heated paraffin embedding station, by Leica (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Dp71 fluorescence microscope, by Olympus (1 mentions) Temed, by Merck Group (1 mentions) Brain heart infusion broth, by BD (1 mentions) Methanol, by Merck Group (1 mentions) Whatman, by Cytiva (1 mentions) Atx224, by Shimadzu (1 mentions)

10 protocols using ajax finechem

Cell Lysis and Protein Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

•

Cell lines cultured in 6 cm diameter round plastic dishes (0.5–1 × 10⁶ cells) or 6-well microtiter plates (0.25–0.5 × 10⁶ cells), ready for harvesting

•

6 cm diameter round plastic dishes, Greiner Bio-One, # 628160, or TPP, # 93060

•

6-well microtiter plates, Nunc, Thermo Fisher Scientific, # 140675, or TPP, # 92406

•

NaCl Ajax Finechem, Thermo Fisher Scientific, # AJA465

•

KCl, Ajax Finechem, Thermo Fisher Scientific, # AJA383

•

Na₂HPO₄, Sigma-Aldrich, # S5136

•

KH₂PO₄, Sigma-Aldrich, # P5379

•

Tris, Formedium, UK, # TRIS01

•

HCl to adjust pH, Ajax Finechem, Thermo Fisher Scientific, # AJA1

•

Glycerol, Ajax Finechem, Thermo Fisher Scientific, # AJA242

•

Sodium dodecyl sulfate (SDS), Sigma-Aldrich, # L3771

•

2-mercaptoethanol (14.3 M), Sigma-Aldrich, # M6250

•

Bromophenol blue Sigma-Aldrich, # B5525

•

Ice chips

•

Dry ice or liquid nitrogen are preferred, otherwise use:

•

Metal rack able to hold 1.5 ml tubes, precooled in −20 °C or −80 °C freezer.

e.g. 80-well Chamber for 1.5 ml tubes, Diversified Biotech, USA, # CHAM-8000, or CoolRack

Thermoconductive Tube Racks, BioCision # M30-PF, also sold by Sigma-Aldrich, # BCS-128

Silva R.C., Castilho B.A, & Sattlegger E. (2017). A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2α. MethodsX, 5, 75-82.

+ Open protocol

+ Expand

Paraffin Embedding for Tissue Sectioning

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sample was decalcified and submerged in 10% EDTA (Ajax Finechem, Thermo Fisher Scientific, Taren Point, Australia; cat no. 17,892). The samples were then processed overnight (Leica TP1020, USA) before being embedded in molten paraffin wax (Leica HistoCore Arcadia H - Heated Paraffin Embedding Station, USA). A 5 m rotary microtome (RM2235, Leica, USA) was used to cut the sections. Flattened paraffin ribbons were collected onto polysine microscope slides (Thermo Scientific) and dried at 60°C for 16 hours (Sakura Heater, Tokyo, Japan).38 (link)

Saskianti T., Nugraha A.P., Prahasanti C., Ernawati D.S., Tanimoto K., Riawan W., Kanawa M., Kawamoto T, & Fujimoto K. (2022). Study of Alveolar Bone Remodeling Using Deciduous Tooth Stem Cells and Hydroxyapatite by Vascular Endothelial Growth Factor Enhancement and Inhibition of Matrix Metalloproteinase-8 Expression in vivo. Clinical, Cosmetic and Investigational Dentistry, 14, 71-78.

+ Open protocol

+ Expand

NF-κB Nuclear Translocation Visualization

Check if the same lab product or an alternative is used in the 5 most similar protocols

This method was used to view NF-κB nuclear translocation. HCE-Ts were seeded on to glass cover slips placed in a 24 well plate (5×10³ cells/well). MSC were seeded on 6.5 µm diameter of Transwells (10⁴ cells/well). The groups and treatments were as described in Table 1. After the treatments, coverslips were fixed in 100% methanol (Ajax Finechem, Thermo Fisher Scientific Australia Pty Ltd) for 10 min at 4°C. Cells were blocked with 10% normal donkey serum (Zymed, Life Technologies, CA, USA)/PBS prior to incubation with rabbit anti-NF-κB or negative control rabbit IgG (Table 6) overnight at 4°C. After rinsing with PBS, cells were incubated with donkey anti-rabbit Alexa Fluor 488 (Table 6) and 1 µg/ml of Hoechst 33342 (Sigma-Aldrich) for 1 h at room temperature (RT). Immunolabelling was visualized and imaged using an inverted Olympus DP71 fluorescence microscope (Olympus, Center Valley, PA, USA).

Wen L., Zhu M., Madigan M.C., You J., King N.J., Billson F.A., McClellan K., Sutton G, & Petsoglou C. (2014). Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Pro-Inflammatory Cytokine-Stimulated Human Corneal Epithelial Cells. PLoS ONE, 9(7), e101841.

+ Open protocol

+ Expand

PHEMA Polymer Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

As described by Lou et al (15 (link)), 1.5 ml of HEMA monomer (Rohm & Haas Company) was injected into a small cylindrical polystyrene mold with a diameter of 15 mm. The monomer was polymerized at 50°C for 20 h, before the mixture was poured into a Soxhlet extractor. Ionized water was used to elute residual monomers and oligomers for 48 h at room temperature (18–20°C). The crosslinking agent EGDMA (Tokyo Kasegi Kogyo Co., Ltd.), the APS initiator (Ajax Finechem; Thermo Fisher Scientific, Inc.), TEMED (Sigma-Aldrich; Merck KGaA) and deionized water were added to the two polymer types, which were prepared in a HEMA sponge, to conduct polymerization. To prepare transparent PHEMA, 101.5 µl EGDMA, 80 µl APS, 40 µl TEMED and 6 g of deionized water were added. For white PHEMA, 36.5 µl EGDMA, 80 µl APS, 40 µl TEMED and 15 g of deionized water were added. The percentage of water in the transparent PHEMA was 29.9 and 74.8% in white PHEMA. Morphological analysis was performed on the surface and on cross-sections of the two polymer types using scanning electron microscopy (magnification, ×1,000).

Lao S., Xu J., Liu Y., Cai S., Lin L., Zhang J., Cai D, & Yin S. (2017). A comparative study of the influence of two types of PHEMA stents on the differentiation of ASCs to myocardial cells. Molecular Medicine Reports, 16(1), 507-514.

+ Open protocol

+ Expand

Isolating and Characterizing P. aeruginosa from Canine Otitis Externa

Check if the same lab product or an alternative is used in the 5 most similar protocols

P. aeruginosa isolates used in this study were obtained from a clinical case of canine otitis externa from Australia. Swabs were cultured onto 5% Columbia sheep blood agar (SBA) (Thermo Fisher Scientific, Scoresby, Vic, Australia) and incubated at 37 °C and 42 °C, overnight. Following incubation, cultures were stained by the Gram-method for determination of purity and morphology and an oxidase test was performed. S. aureus ATCC 29213 was routinely streaked out onto SBA and incubated at 37°C overnight. All bacterial isolates were stored at −80 °C in brain heart infusion broth (BD Biosciences, Sydney, NSW, Australia) with 20% glycerol (Ajax Finechem (Thermo Fisher)).

Jeffery Marano R., Jane Wallace H., Wijeratne D., William Fear M., San Wong H, & O’Handley R. (2015). Secreted biofilm factors adversely affect cellular wound healing responses in vitro. Scientific Reports, 5, 13296.

+ Open protocol

+ Expand

Herbal Extract Preparation for In Vitro Testing

Check if the same lab product or an alternative is used in the 5 most similar protocols

C168 in powder form was provided by Inchoice Technology Sdn. Bhd. (Selangor, Malaysia). C168 consisted of seven genera of Chinese herbs, namely, Cinnamomum spp., Zingiber spp., Atractylodes spp., Carthamus spp., Angelica spp., Curcuma spp., Glycyrrhiza spp., and Astragalus spp. C168 powder was macerated with methanol (Merck, Malaysia) for 72 hours and filtered using a Whatman filter paper. The solvent was dried by rotary evaporation, followed by freeze drying. The extract was then reconstituted in DMSO (Ajax Finechem, Thermo Fisher Scientific) to prepare 800 mg/mL stock solution for in vitro testing.

Leong L.M., Chan K.M., Hamid A., Latip J, & Rajab N.F. (2016). Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage. Evidence-based Complementary and Alternative Medicine : eCAM, 2016, 2091085.

+ Open protocol

+ Expand

Preparation of NaCl/H2O Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

NaCl/H₂O samples are prepared on a gravimetric basis using NaCl (Ajax Finechem, Thermo Fisher Scientific, minimum assay 99.7%) and deionised water (Pacific TII 12, Thermo Scientific). The balance has an accuracy of 1 mg (ATX224, Shimadzu). Artificial seawater was prepared using the same apparatus and procedure with rock sea salt sourced from Whyalla, South Australia (Rock sea salt, Olsson’s Salt), which was harvested through solar evaporation with no additives.

Xu S., Hutchinson A.J., Taheri M., Corry B, & Torres J.F. (2024). Thermodiffusive desalination. Nature Communications, 15, 2996.

+ Open protocol

+ Expand

Periodontal Histological Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

In order to assess the samples’ periodontal conditions, maxillae were dissected at 0, 7, and 14 days and subsequently fixed with 10% formaldehyde. Demineralization was performed according to Ayukawa et al. (1998) [64 (link)]. Briefly, both sides of each maxilla were decalcified with 10% ethylene-diamine-tetra-acetic acid solution (pH 7.0) (Ajax Finechem, Thermo Fisher Scientific; Taren Point, Australia) [65 (link)] for 4 weeks at 4 °C. Then tissue blocks were embedded in paraffin and cut into serial mesial-distal sections (5 μm thick) using an ultramicrotome (Bright 5040, Bright Instrument, Cambs, UK). After staining with hematoxylin and eosin (H&E) (HD Scientific Supplies; Wetherill Park, Australia) [65 (link)], histological images were acquired using a microscope slide scanner (OPTIKA, Ponteranica, Italia) [63 (link)].

Lin S.K., Wu Y.F., Chang W.J., Feng S.W, & Huang H.M. (2022). The Treatment Efficiency and Microbiota Analysis of Sapindus mukorossi Seed Oil on the Ligature-Induced Periodontitis Rat Model. International Journal of Molecular Sciences, 23(15), 8560.

+ Open protocol

+ Expand

Isolation and Culture of Human Lung Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue isolation was performed on fresh explanted lung tissues. Human lung parenchymal tissues were isolated and cut into pieces no bigger than 1 cm³. These pieces were fixed in 4% formaldehyde (Fronine; Thermo Fisher Scientific, Waltham, MA, USA). After formaldehyde fixation, isolated lung tissues were embedded in paraffin.
Primary human lung fibroblasts were isolated as previously described (Krimmer et al., 2012 (link); White et al., 2003 (link)) and were grown in growth media [Dulbecco's modified Eagle's medium (DMEM) low glucose (Gibco, Thermo Fisher Scientific) with 0.025 M HEPES (Gibco), 0.375% sodium hydrogen carbonate (Ajax Finechem, Thermo Fisher Scientific), 10% (v/v) FBS (JRH Biosciences, Brooklyn, Victoria, Australia) and 1% (v/v) antibiotics (Invivogen, San Diego, CA, USA), pH 7.1-7.2]. All fibroblast cell lines were tested for mycoplasma contamination after cell isolation and culture. For experiments, mycoplasma-free fibroblasts were used between the third and ninth passages.

Tjin G., White E.S., Faiz A., Sicard D., Tschumperlin D.J., Mahar A., Kable E.P, & Burgess J.K. (2017). Lysyl oxidases regulate fibrillar collagen remodelling in idiopathic pulmonary fibrosis. Disease Models & Mechanisms, 10(11), 1301-1312.

+ Open protocol

+ Expand

Primary Lung Fibroblast Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary human lung fibroblasts were grown in growth media [DMEM low glucose (Gibco) with 0.025 M HEPES (Gibco) and 0.375% sodium hydrogen carbonate (Ajax Finechem, Thermo Fisher Scientific)] incubated in a humidified CO₂ incubator (5% CO₂ in air) at 37°C. Growth media were aspirated from cells every 4 days and replaced with fresh growth media until confluence.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!