High-resolution mass spectra were recorded on an Agilent Technologies G6520A Q-TOF mass spectrometer using electrospray ionization. NMR spectra were recorded on Bruker Avance I and III spectrometers (400 or 500 MHz), and chemical shifts are expressed in parts per million (δ) relative to chloroform (7.26 ppm for 1H and 77.2 ppm for 13C) or DMSO (2.50 ppm for 1H and 39.5 ppm for 13C) as an internal reference. TLC was performed on 0.25-μm silica gel 60 F254 aluminum-backed plates. Silica gel 60 (230–400 mesh) was used for flash column chromatography. The compounds were visualized by UV light and by spraying with 10% phosphomolybdic acid in ethanol. THF and toluene were distilled from sodium and benzophenone. D-erythro-Sphingosine, N-stearoyl-D-erythro-sphingosine, and N-lignoceroyl-D-erythro-sphingosine were purchased from Avanti Polar Lipids. Flash column chromatography for 10 –15 was performed using a Biotage Isolera One automated system with Flash KP-Sil™ silica cartridges. Microwave-assisted experiments were carried out in a microwave instrument (Smithcreator™) in Pyrex vials using standard procedures.
G6520a q tof mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The G6520A Q-TOF mass spectrometer is a high-resolution, accurate-mass instrument designed for precise and sensitive analysis of a wide range of analytes. It utilizes quadrupole time-of-flight (Q-TOF) technology to provide accurate mass measurements and structural information.
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3 protocols using g6520a q tof mass spectrometer
1
Characterization of Sphingoid Lipids
2
Chromatographic Characterization of Organic Compounds
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Commercially available reagents were used for the reaction. Column chromatography was performed using silica gel grade 60 (230–400 mesh). All solvents used for the reaction were commercially available anhydrous solvents. The 1H-NMR and 13C-NMR were measured using JEOL ECZ500R (JEOL Co., Tokyo, Japan), and deuterated solvents at 500 and 125 MHz, respectively. An Agilent Technologies G6520A Q-TOF mass spectrometer (Santa Clara, CA, USA) instrument using electrospray ionization (ESI) was used to measure high-resolution mass spectra.
3
Spectroscopic Characterization of Compounds
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Reagents and solvents used for the reaction were purchased commercially. Silica gel used for column chromatography was grade 60 (230–400 mesh). 1H-NMR and 13C-NMR were measured using JEOL ECZ500R (JEOL Co., Tokyo, Japan) at 500 and 125 MHz, respectively, and a deuterated solvent was used. High-resolution mass spectra were measured using an Agilent Technologies G6520A Q-TOF mass spectrometer (Santa Clara, CA, USA) using electrospray ionization (ESI).
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