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Hiperfect agent

Manufactured by Qiagen
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The HiPerFect agent is a transfection reagent designed for efficient delivery of small interfering RNA (siRNA) or other nucleic acids into a variety of cell lines. It facilitates the uptake of these molecules into cells, enabling effective gene silencing or other applications that require the introduction of nucleic acids.

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Lab products found in correlation

Sidkk3, by Qiagen (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

2 protocols using hiperfect agent

1

miRNA Mimics Transfection Protocol

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HiPerFect agent (Qiagen) was used to deliver miRNA mimics into cells maintained in 6-well plates. According to the manufacturer’s instruction, cells were grown to ~70% confluence and 20 nM miRNA mimics and 12 μl of transfection agent was mixed thoroughly in 100 μl of Opti-MEM. After 10 min at room temperature, the mixture was added into cells. The subsequent experiments were performed 48 h after the transfection.
YE Z.B., MA G., ZHAO Y.H., XIAO Y., ZHAN Y., JING C., GAO K., LIU Z.H, & YU S.J. (2014). miR-429 inhibits migration and invasion of breast cancer cells in vitro. International Journal of Oncology, 46(2), 531-538.
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2

Silencing DKK3 in Human Pancreatic Stem Cells

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Human pcDNA3.1/V5-His A-DKK3 construct was provided by L.Zhang (Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, Pittsburgh, PA) (60 (link)). DKK3 was silenced by lentiviral transfection with shDKK3 (Open Biosystems) and by transfection with siDKK3 (Qiagen). Stable silencing of DKK3 in HPSCs was achieved by cotransfection of lentiviral plasmid control vector or shDKK3 (HPSC-shControl or HPSC-shDKK3) with packaging vectors into 293T cells with Lipofectamine 2000 (Invitrogen). Viral supernatant (200 μl) was added to HPSCs with polybrene (8 μg/ml; hexadimethrine bromide) in a six-well plate for 2 days, and stably transduced cells were selected in puromycin (1 μg/ml). Transient silencing of DKK3 in HPSCs and PDAC cells was achieved by transfection with a mixture of 5 nM siDKK3 and 3 μl of HiPerFect agent according to the manufacturer’s recommendations (Qiagen).
Zhou L., Husted H., Moore T., Lu M., Deng D., Liu Y., Ramachandran V., Arumugam T., Niehrs C., Wang H., Chiao P., Ling J., Curran M.A., Maitra A., Hung M.C., Lee J.E., Logsdon C.D, & Hwang R.F. (2018). Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma. Science translational medicine, 10(464), eaat3487.
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