Cary 300 uv vis double beam spectrophotometer

Manufactured by Agilent Technologies

The Cary 300 UV-Vis double-beam spectrophotometer is a laboratory instrument designed for the measurement of the absorption or transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It features a double-beam optical system, allowing for simultaneous measurement of a sample and a reference.

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Cary 300 UV-Vis spectrophotometer Cary 300 UV-Vis Cary UV/Vis spectrophotometer Cary 300 UV spectrophotometer Cary 300 spectrophotometer UV-Vis spectrophotometer Cary 300 UV spectrophotometer

4 protocols using cary 300 uv vis double beam spectrophotometer

UV-Vis Absorption Spectra of Solutions

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UV-Vis absorption spectra for solutions A – D were measured at room temperature using a Cary 300 UV-Vis double-beam spectrophotometer. Samples were measured in quartz cells with 1 mm path-length, using a 1.5 absorbance Agilent rear-beam attenuator (RBA) mesh filter in the reference position.

Sisombath N.S., Jalilehvand F., Schell A.C, & Wu Q. (2014). Lead(II) Binding to the Chelating Agent d-Penicillamine in Aqueous Solution. Inorganic Chemistry, 53(23), 12459-12468.

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UV-vis Spectroscopy of Rhodium Complexes

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A Cary 300 UV-vis double-beam spectrophotometer was used to measure the UV-vis spectra of Rh₂(AcO)₄ and glutathione solution mixtures (for sample preparation, see Section 2.1). Quartz cells with a path length of 1 mm were used to hold the samples, using water as blank.

Garcia A.E, & Jalilehvand F. (2017). Aerobic Reactions of Antitumor Active Dirhodium(II) Tetraacetate Rh2(CH3COO)4 with Glutathione. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 23(2), 231-239.

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UV-Vis Absorption Spectra Measurement

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UV-vis absorption spectra for solutions A–D
were measured at room temperature, using a Cary 300 UV-vis double-beam
spectrophotometer. Samples were measured in quartz cells with a path
length of 1 mm, using a 1.5 absorbance Agilent rear-beam attenuator
(RBA) mesh filter in the reference position.

Sisombath N.S., Jalilehvand F., Schell A.C, & Wu Q. (2014). Lead(II) Binding to the Chelating Agent d-Penicillamine in Aqueous Solution. Inorganic Chemistry, 53(23), 12459-12468.

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Spectrophotometric Analysis of Gel Samples

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The gel samples were directly prepared into disposable cuvettes with 10 mm optical path. The spectrophotometric analyses were performed using a Cary 300 UV-vis double beam spectrophotometer, using a cuvette with the solvent as reference.

Ravarino P., Di Domenico N., Barbalinardo M., Faccio D., Falini G., Giuri D, & Tomasini C. (2022). Fluorine Effect in the Gelation Ability of Low Molecular Weight Gelators. Gels, 8(2), 98.

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