Maldi tof ms system

Manufactured by bioMérieux

Sourced in France

The MALDI-TOF MS system is a laboratory instrument that uses matrix-assisted laser desorption/ionization (MALDI) and time-of-flight (TOF) mass spectrometry to analyze and identify various types of biological samples, such as microorganisms, proteins, and peptides.

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Lab products found in correlation

Vitek 2 compact, by bioMérieux (1 mentions) Vitek ms system, by bioMérieux (1 mentions) Brain heart infusion broth, by Thermo Fisher Scientific (1 mentions) Api 20e system, by bioMérieux (1 mentions) Vitek 2, by bioMérieux (1 mentions) Vitek 2 compact system, by bioMérieux (1 mentions) Vitek 2 system, by bioMérieux (1 mentions) Bactec blood culture system, by BD (1 mentions) Bact alert 3d, by bioMérieux (1 mentions) Vitek ms matrix, by bioMérieux (1 mentions)

Spelling variants of this product

MALDI-TOF MS (184 citations) MALDI-TOF (49 citations) VITEK MS MALDI-TOF system (10 citations) MALDI-TOF VITEK MS® 3.0 system (2 citations) MALDI-TOF VITEK MS Microbial Identification System (2 citations)

15 protocols using maldi tof ms system

Ocular Fungal Infection Diagnostic Protocol

Cited 3 times

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Specimens were taken in the microbiological lab in our hospital under topical anesthesia (0.5%, proparacaine hydrochloride) or during operation. Protocols were conducted following our established methods published previously.27 (link) Secretions of the conjunctival sac were collected by sterile cotton swabs; cornea specimens were sampled by scraping the base and edges of the pathological lesions; and anterior chamber fluids and vitreous tissue were obtained by syringe aspiration or vitrectomy in an operating room. Most specimens were simultaneously tested for bacteria and fungi. This study only evaluated the ocular fungal infection over ten years.
The collected specimens were tested by direct smears and culturing by established methods.27 (link),28 Fungal culture was conducted using potato dextrose agar medium for 7 days or more at 28°C. Positive fungal findings were evaluated based on colony morphology, growth characteristics and microscopic features with staining following a lactophenol cotton blue mount preparation.29 (link),30 (link)
Bacterial cultures were made in nutrient broth for up to 7 days at 37°C and after in sheep blood agar for 24 hours at 37°C.31 (link) Then, bacterial isolates were identified by an automated microbiology system (Vitek 2 Compact, BioMerieux, Inc. 100 Rodolphe Street, Durham, USA) or by a MALDI-TOF-MS system (BioMerieux).

Pei Y., Chen X., Tan Y., Liu X., Duan F, & Wu K. (2022). Microbiological Profiles of Ocular Fungal Infection at an Ophthalmic Referral Hospital in Southern China: A Ten-Year Retrospective Study. Infection and Drug Resistance, 15, 3267-3276.

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Comprehensive Acinetobacter baumannii Isolation

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A total of 536 nonduplicate A. baumannii isolates was collected from 20 hospitals in 13 provinces and cities in China between January 2018 and December 2019. These A. baumannii isolates were isolated from sputum (69.6%), bronchial alveolar lavage fluid (4.3%,), blood (6.9%,), secreta (4.5%), urine (3.2%), pleural fluid (2.8%), cerebrospinal fluid (2.1%), ascites (1.9%), pus (1.3%), bile (0.9%), catheter (0.4%), drainage (0.4%), aseptic body fluid (0.4%), and other sources (1.5%). Species identification was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) system (bioMérieux, France).

Han R., Ding L., Yang Y., Guo Y., Yin D., Wu S., Zhi P., Zhu D., Liu Q., Tan X., Zhu Y., Zhang J., Li L, & Hu F. (2022). In Vitro Activity of KBP-7072 against 536 Acinetobacter baumannii Complex Isolates Collected in China. Microbiology Spectrum, 10(1), e01471-21.

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Diverse KPC-2 Variants in K. pneumoniae

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Nineteen K. pneumoniae carrying bla_KPC-2 variants were collected at Huashan Hospital (Shanghai, China) during 2019 to 2021, isolated from sputum, urine, abdominal fluid, and cerebrospinal fluid. Species identification was confirmed by a MALDI-TOF/MS system (bioMérieux, France). The presence and subtypes of the bla_KPC-2 gene were initially confirmed by PCR-based DNA sequencing, including whole-genome sequencing using Illumina (Illumina, San Diego, CA, USA), and were compared with available sequences in GenBank. An additional antimicrobial resistance gene analysis was performed using ResFinder 4.1 (https://cge.cbs.dtu.dk/services/ResFinder/). Among those bla_KPC-2 variants, bla_KPC-33-, bla_KPC-35-, bla_KPC-71-, bla_KPC-76-, bla_KPC-78-, and bla_KPC-79-positive isolates accounted for 26.3% (5/19), 15.8% (3/19), 5.3% (1/19), 42.1% (8/19), 5.3% (1/19), and 5.3% (1/19), respectively. K. pneumoniae ATCC BAA-1705, K. pneumoniae ATCC BAA-2146, E.coli ATCC 25922, respectively, were tested as a KPC-positive strain, NDM-positive strain, and carbapenemase-negative strain for quality control in carbapenemase detection. In addition, E.coli ATCC 25922 was included for a quality control assessment in antimicrobial susceptibility testing.

Ding L., Shi Q., Han R., Yin D., Wu S., Yang Y., Guo Y., Zhu D, & Hu F. (2021). Comparison of Four Carbapenemase Detection Methods for blaKPC-2 Variants. Microbiology Spectrum, 9(3), e00954-21.

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Identifying Fecal Microbiome Composition

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The feces of both OVA/alum mice and PBS/alum mice were suspended in 20 volumes of PBS, inoculated onto plates containing Columbia agar with 5% sheep blood, and cultured anaerobically for 2 days at 37°C. To identify viable bacteria in the feces of the mice, a portion of each fresh colony that developed on each plate was smeared onto a target slide as a sample. These samples were covered with α-cyano-4-hydroxycinnamic acid matrix solution and loaded into a Vitek MALDI-TOF MS system (bioMérieux, Lyon, France). The spectra of the samples were analyzed using the Myla database to identify the species of origin (42 (link)). A total of 48 fresh colonies from each group of mice were analyzed.

Matsui S., Kataoka H., Tanaka J.I., Kikuchi M., Fukamachi H., Morisaki H., Matsushima H., Mishima K., Hironaka S., Takaki T., Okahashi N., Maruoka Y, & Kuwata H. (2019). Dysregulation of Intestinal Microbiota Elicited by Food Allergy Induces IgA-Mediated Oral Dysbiosis. Infection and Immunity, 88(1), e00741-19.

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Bacterial Identification using MALDI-TOF MS

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The bacterial isolates were recovered and grown on Mueller Hinton agar for 24 h at 37 °C for obtaining isolated colonies. The identification was accomplished using Vitek MS system (Matrix-assisted laser desorption ionization time of flight mass spectrometry - MALDI-TOF MS system - BioMerieux) in accordance with the manufacturer’s instructions. Escherichia coli strain ATCC™ (American Type Culture Collection) 8739 was used as a positive control. The Myla® database was accessed for the identification of bacterial isolates. A confidence level greater than 80% was adopted for genus assignment and greater than 90% for species.

Pereira A.L., de Oliveira P.M., Faria-Junior C., Alves E.G., de Castro e Caldo Lima G.R., da Costa Lamounier T.A., Haddad R, & de Araújo W.N. (2022). Environmental spreading of clinically relevant carbapenem-resistant gram-negative bacilli: the occurrence of blaKPC-or-NDM strains relates to local hospital activities. BMC Microbiology, 22, 6.

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Decontamination Efficacy of Face Masks

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Inoculated segments (1-cm² squares) were excised from treated FFR using sterile scissors and placed into 10 mL brain heart infusion broth (Thermo Fisher). Colony counts were obtained as follows: inoculated tubes were placed in an orbital shaker (4°C for 5 minutes at 250 rpm), mixed with a vortexer (5 seconds), placed in 1-mL aliquots on a Columbia sheep blood agar plate, and incubated for 18–24 hours at 37°C in 5% CO₂. The remaining broth was incubated (48 hours at 37°C) and subcultured onto Columbia sheep blood agar if turbid. Colonies were identified using the VITEK matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) system (bioMérieux, Marcy-l'Étoile, France). Decontamination efficacy, defined as the percentage reduction in colony-forming units (CFU), was determined by comparing colony counts cultured from treated and untreated FFR segments. The effectiveness of sterilization was determined using Health Canada guidance (e.g. minimum 6-log reduction) (FDA 2020).²¹

Clark S., Ng H., Wu G., Jain D., Bassi G., Kandel R, & Mazzulli T. (2021). Comparative evaluation of four hydrogen peroxide-based systems to decontaminate N95 respirators. Antimicrobial Stewardship & Healthcare Epidemiology : ASHE, 1(1), e21.

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Multidrug-resistant E. cloacae Isolation

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E. cloacae BF1417 was recovered during an epidemiological study at the Military Hospital of Tunis, Tunisia. This strain was isolated from a stool culture of a 75-year-old man hospitalized for renal failure in the intensive care unit at the Military hospital of Tunis. The studied strain was selected on the basis of its multidrug-resistance phenotype (MDR) and it was identified using MALDI-TOF MS system, the VITEK 2 (bioMérieux, La Balme-les-Grottes, France) and the API 20 E system (bioMérieux, Marcy l’Etoile, France). E. coli DH5α competent cells were used as host for cloning and for the transformation experiments. Streptomycin-resistant E. coli HB101 was used as a recipient for conjugation tests.

Bourouis A., Ben moussa M, & Belhadj O. (2015). Multidrug-resistant phenotype and isolation of a Novel SHV- beta-Lactamase variant in a clinical isolate of Enterobacter cloacae. Journal of Biomedical Science, 22(1), 27.

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Antimicrobial Resistance Profiling of K. pneumoniae

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K. pneumoniae isolates were identified using the VITEK 2 Compact system (bioMérieux, France) or matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) system (bioMérieux). The antimicrobial resistance of isolates was detected by antimicrobial sensitivity tests using VITEK 2 Compact system and disc diffusion method on the Mueller-Hinton (MH) Agar plates. These antibiotics included ceftriaxone, ciprofloxacin, levofloxacin, tigecycline, amikacin, imipenem, meropenem, ceftazidime/avibactam, piperacillin/tazobactam, amoxicillin/clavulanate, cefoperazone/sulbactam, aztreonam, gentamicin, cefepime, cotrimoxazole, and tobramycin. The antimicrobial susceptibility of tigecycline was interpreted according to Food and Drug Administration (FDA). The antimicrobial susceptibility of other antibiotics was interpreted according to Clinical and Laboratory Standards Institute (CLSI).

Fang Y., Zhong Q., Chen Y., Hang Y., Fang X., Xiao Y., Cao X., Zhu H., Luo H., Peng S., Gu S., Li F., Zhu J., Xiong J, & Hu L. (2023). Ceftazidime/Avibactam, Polymyxin or Tigecycline as a Rescue Strategy for the Treatment of Carbapenem-Resistant Klebsiella pneumoniae in Bloodstream Infection: A Retrospective Cohort Study. Infection and Drug Resistance, 16, 2963-2971.

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Characterization of CZA-resistant CRPA

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A CZA-resistant CRPA strain, designated PA2207, was recovered from fecal samples from a patient who suffered from leukemia during the process of screening for carbapenem-resistant Enterobacteriaceae (CRE) screening. The patient had previously been treated with multiple antimicrobials, including CZA. The strain was identified by the MALDI-TOF MS system (bioMérieux, Marcy l'Etoile, France) and further confirmed by whole-genome sequencing.

Tu Y., Wang D., Zhu Y., Li J., Jiang Y., Wu W., Li X, & Zhou H. (2022). Emergence of a KPC-90 Variant that Confers Resistance to Ceftazidime-Avibactam in an ST463 Carbapenem-Resistant Pseudomonas aeruginosa Strain. Microbiology Spectrum, 10(1), e01869-21.

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Evaluating Tolomea Resistance in Multidrug-Resistant P. aeruginosa

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This study was conducted on 504 clinical isolates of P. aeruginosa with the so called multidrug resistant (MDR) phenotype. Isolates were collected from 20 medical institutions located in Colombia, Brazil, Argentina, Chile, and Mexico from January 2016 to October 2017, prior to the introduction of TOL into the clinical practice in these countries. MDR was defined by non-susceptibility to ≥1 agent in ≥3 classes that are typically active against P. aeruginosa (Magiorakos et al., 2012 (link)).
Bacterial identification was performed using the MALDI-TOF MS system (BioMérieux, France). Minimum inhibitory concentrations (MICs) for TOL were determined by broth microdilution, following the guidelines of the Clinical and Laboratory Standard Institute (CLSI) M100 (CLSI, 2021 ). Escherichia coli ATCC 25922 and P. aeruginosa ATCC 27853 were used as controls.

Mojica M.F., De La Cadena E., Ríos R., García-Betancur J.C., Díaz L., Reyes J., Hernández-Gómez C., Radice M., Gales A.C., Castañeda Méndez P., Munita J.M., Pallares C.J., Martínez J.R, & Villegas M.V. (2022). Molecular mechanisms leading to ceftolozane/tazobactam resistance in clinical isolates of Pseudomonas aeruginosa from five Latin American countries. Frontiers in Microbiology, 13, 1035609.

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