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20 protocols using 5d mark 2

1

Photographic Documentation of Insect Specimens

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Photographs of specimens deposited within the IMQC, Coll SLT, and Coll TM were taken by René Limoges (IMQC) using a Nikon D850 DSLR camera (Nikon Corporation, Tokyo, Japan) with Nikon Micro-Nikkor 200 mm f/4 lens on Manfrotto 454 micrometric positioning sliding plate (Manfrotto, Casolla, Italy). Lighting was provided by two Nikon SB-25 flash units with a Cameron Digital diffusion photo box (Henry’s, Vancouver, Canada). Photographs of specimens within the first authors collection (Coll RC) were taken by the first author using a Canon 5D Mark II and a MP-E 65 mm macro lens and stacked using Zerene Stacker (Zerene Systems LLC, Richland, USA). Photographs not taken by René Limoges (IMQC) are accompanied by citation to their photographers and were taken using a variety of equipment. All images were edited using Adobe Photoshop Elements 13 (Adobe Inc., San Jose, USA) to remove their backgrounds and correct brightness/contrast. For wing illustrations (Figs 3, 4) specimens were chosen with the tegmina and alae already pinned flat, and the wings were photographed and simply cropped in the photos from the bodies of the specimens, not dissected from the specimens. Wing venation terminology follows Burt (1932) and Ragge (1955) (link).
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2

Standardized Skin Imaging Protocol

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The image acquisition environment must be set in advance to proceed with the forward model. There are two factors: a light source and camera sensitivity. In this study, skin images were acquired using the “VISIA-CR” system. To ensure consistency between image acquisition and analysis conditions, the spectral data of the standard illuminant “D65” in ISO 2002 and the camera sensitivity of the “Canon 5D Mark II36 (link),37 (link) within the wavelength range of 400 to 720 nm are used. The spectrum of the light source is normalized by inversely multiplying a scaling factor, which is derived by multiplying the light source and the maximum sensitivity value of the camera channels.
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3

Examination of Male Genitalia Structures

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The genital segments of the specimens were soaked in 10% NaOH overnight and observed or drawn in glycerine using a Leica MZ125 (Leica, Germany) stereomicroscope. The genital segments were then preserved in glycerine in 0.20 ml centrifuge tubes. Photographs of partial body of male were taken by Canon 5D Mark II digital single lens reflex camera (Canon, Japan) with MP-E 65mm f/2.8 1-5X macro lens (Canon, Japan). All measurements are in millimeters (mm), made with the aid of a digital caliper. The terminology and methods of description and illustration follow those ofPageBreak
Alexander and Byers (1981) and Frommer (1963) . The type specimens of the new species are deposited in the animal specimen room, School of Life Sciences, Anqing Normal University, Anqing, Anhui Province, China.
The key was principally constructed from descriptions in the literature without examination of the type species of most of these species, and should be considered preliminary. The characters used in the key rely primarily on the structures of genitalia and the length of antenna of male specimens.
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4

Structural Color Hydrogel Characterization

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Reflection spectra were acquired at a constant glancing angle employing an optical microscope equipped with a fiber-optic spectrometer (Ocean Optics, USB2000-FLG). The spectra of the structural color hydrogels were measured before water volatilization. The light beam size for the reflectance measurement is ~0.636 mm2. SEM images were obtained by scanning electron microscopy (SEM, Hitachi S-3000N). Optical images were taken using an optical microscope (Olympus BX51) equipped with a CCD camera (Media Cybernetics Evolution MP5.0) and a digital camera (Canon5D Mark II, Japan).
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5

Detailed Morphological Documentation Protocol

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Legs were drawn on squared paper using a Reichert binocular microscope with an ocular screen. Details of the male and female genitalia and all preimaginal stages were drawn by means of an Abbe's drawing apparatus on a compound microscope (JENAVAL) at larger magnification (130-500 ×). Wings were photographed using an Olympus BX51 compound microscope with an attached digital camera (Canon EOS 1200D). Whole adult specimens and their details were photographed by P. Krásenský by means of a Canon 5D Mark II digital camera with a Nikon CFI Plan 10 × / 0.25NA 10.5 mm WD objective attached on a Canon EF 70-200 mm f / 4L USM lens. The specimens were moved upwards between each exposure using a WeMacro Rail (http://www.wemacro.com/, each step was 0.01 mm) and the final photographs were stacked from multiple layers using Zerene Stacker. The final images were edited in Adobe Photoshop CS6.
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6

Imaging Polytrichadelphus Moss Specimens

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Records of two Chilean specimens of P. hammoniorum were performed on native Polytrichadelphus moss at room temperature, using a Canon 5D Mark II camera (30 frames per second), MP-E 65mm lens and Macro Ring Lite MR-14EX cold light source.
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7

Characterization of Graphene Aerogel Foam

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SEM images were obtained using an SEM (Hitachi S-3000N). Color photos and videos were taken on a digital camera (Canon 5D Mark II). Raman spectroscopy was conducted using a Raman microscope (RAMAN, inVia, Renishaw) with a 532-nm laser to analyze the surface physicochemical structure of the products. The mechanical strength of the graphene aerogel foam was tested using an Instron 5943 single-column testing machine (ITW) with a load cell capacity of 1 kN. The loading process was displacement-controlled, and the loading rate was set to be 3 mm min−1. DSC thermograms were measured using DSC 8000 (PerkinElmer), with second heating curves and cooling curves at heating and cooling rates of 10°C min−1. The resistance of the graphene foam was measured by a semiconductor characterization system (4200-SCS/F, Keithley). Water contact angles were obtained by a JC2000D2 contact angle measuring system at ambient temperature. Sliding angles were measured on a customized tilting stage with a droplet volume of 6 μl. Static contact angles were recorded with a droplet volume of 2 μl. The static contact angles were measured at a neutral tilting angle (0°). The tested liquids were hexane (18.43 mN m−1), heptane (20.14 mN m−1), octane (21.6 mN m−1), decane (23.7 mN m−1), dodecane (25.4 mN m−1), hexadecane (27.3 mN m−1), ethylene glycol (48.1 mN m−1), glycerol (60.3 mN m−1), and water (72.4 mN m−1).
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8

Multifunctional Microneedle Patch Fabrication

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HAMA hydrogel (0.1 g mL−1), HMPP (1%, v/v), and PDA NPs (80 µg) were mixed in aqueous solution to make the outer layer of the tips. After drying overnight and solidifying the tips through UV irradiation for 60 s, HA (0.3 g mL−1) and Fe‐MSC‐NVs (50 µg) were entirely filled into the quadrangular pyramid microcavities of the mold to prepare the tip solution and dried for 12 h. The backing layer solution (0.3 g mL−1 HA hydrogel) was then filled and dried for 12 h. The resultant MN patch was obtained by demolding. The optical images of MN array were obtained through a Canon 5D Mark II digital camera. SEM photos were taken by a Zeiss Ultra Plus SEM. The CLSM images were taken by an Olympus FV3000 confocal microscope using fluorescent dyes as a demonstration, with red‐microspheres (≈50 nm) in the outer HAMA layer and DiO‐labeled Fe‐MSC‐NVs loaded in the inner HA layer.
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9

Microscopic Imaging and Digital Photography

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We photographed sections using an Olympus SZX12 microscope equipped with an Olympus DP70 digital camera. We used a Canon 5D Mark II digital camera to record color macrophotographs. Using
Adobe Photoshop CS6, we adjusted images for color balance and contrast, and prepared plates and line drawings in Adobe Illustrator CS6.
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10

Salp Fecal Pellet Morphometrics

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The >200-μm mesh filters for each PIT tube (used for removing zooplankton “swimmers”) were imaged using a Canon 5D Mark II camera with attached 100 mm F/2.8 macro lens mounted in a downward-facing macrophotography rig. Images were manually analyzed using Image J to determine morphometric measurements for each large salp fecal pellet (FP). Morphometric measurements were then used to estimate FP volume and carbon content.
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