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Biospa live cell analysis system

Manufactured by Agilent Technologies
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The BioSpa Live Cell Analysis System is a laboratory instrument designed for continuous, automated monitoring of live cell cultures. The system integrates imaging, environmental control, and data analysis capabilities to enable long-term observation and measurement of cellular processes.

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Lab products found in correlation

Comet assay kit, by R&D Systems (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions) Cometassay single cell gel electrophoresis assay, by Bio-Techne (1 mentions)

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BioSpa (7 citations)

5 protocols using biospa live cell analysis system

1

Comet Assay Analysis of Topotecan and M6620

Cited 1 time
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DMS114 cells were treated 1 μM topotecan or 1 μM topotecan + 1 μM M6620 for 8 h. Cells were then collected for alkaline comet assays using the CometAssay Kit (R&D Systems®, Catalog # 4250–050-K) following manufacturer’s instructions. Images were captured using BioSpa Live Cell Analysis System (Biotek) and tail moment was calculated using OpenComet(Gyori et al., 2014 (link)), a plugin for the image processing program ImageJ.
Thomas A., Takahashi N., Rajapakse V.N., Zhang X., Sun Y., Ceribelli M., Wilson K.M., Zhang Y., Beck E., Sciuto L., Nichols S., Elenbaas B., Puc J., Dahmen H., Zimmermann A., Varonin J., Schultz C.W., Kim S., Shimellis H., Desai P., Klumpp-Thomas C., Chen L., Travers J., McKnight C., Michael S., Itkin Z., Lee S., Yuno A., Lee M.J., Redon C.E., Kindrick J.D., Peer C.J., Wei J.S., Aladjem M.I., Figg W.D., Steinberg S.M., Trepel J.B., Zenke F.T., Pommier Y., Khan J, & Thomas C.J. (2021). Therapeutic targeting of ATR yields durable regressions in small cell lung cancers with high replication stress. Cancer cell, 39(4), 566-579.e7.
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2

Immunofluorescence Staining of Melanoma Cells

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PIG1 and PIG3V were seeded and treated in 12-well plates. After being washed and fixed, cells were incubated with melanoma gp100 (Abcam, UK) antibody overnight at 4°C. Following three washes with PBS, cells were incubated with the goat antirabbit Alexa Fluor® 594 (IgG H&L) antibody (Abcam, USA) for an additional 1h in darkness. After rinsing with PBS, Phalloidin (Cell Signaling Technology, USA) were added to the cells. Then cells were incubated for 30 min at room temperature, and nuclear dyed with DAPI (Solarbio, China) for 10 min at room temperature. Fluorescent images were obtained by the BioSpa Live Cell Analysis System (BioTek, USA).
Yuan J., Lu Y., Wang H., Feng Y., Jiang S., Gao X.H., Qi R., Wu Y, & Chen H.D. (2020). Paeoniflorin Resists H2O2-Induced Oxidative Stress in Melanocytes by JNK/Nrf2/HO-1 Pathway. Frontiers in Pharmacology, 11, 536.
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3

Comet Assay for DNA Damage Assessment

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DMS 114 cells were plated at 500 K cells per well in a six‐well plate, and cells were treated with ±1 nM lurbinectedin and ±2 μM berzosertib for 6 h and collected. Comet assays were performed using the Comet Assay Single Cell Gel Electrophoresis Assay (Trevigen, Gaithersburg, MD) according to manufacturer's instructions. Images were captured using BioSpa Live Cell Analysis System (Biotek) and comet tail length was calculated using OpenComet (https://cometbio.org/), a plugin for the image processing program ImageJ.
Schultz C.W., Zhang Y., Elmeskini R., Zimmermann A., Fu H., Murai Y., Wangsa D., Kumar S., Takahashi N., Atkinson D., Saha L.K., Lee C., Elenbaas B., Desai P., Sebastian R., Sharma A.K., Abel M., Schroeder B., Krishnamurthy M., Kumar R., Roper N., Aladjem M., Zenke F.T., Ohler Z.W., Pommier Y, & Thomas A. (2023). ATR inhibition augments the efficacy of lurbinectedin in small‐cell lung cancer. EMBO Molecular Medicine, 15(8), e17313.
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4

Neutral Comet Assay for DNA Damage

Cited 1 time
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Neutral comet assays were performed using previously described protocol with minor modifications35 (link). In brief, HCT116 cells were treated 20 μM SN38 for 2 h. Cells were then collected for neutral comet assays using the CometAssay Kit (R&D Systems, Catalog # 4250-050-K) following manufacturer’s instructions. Images were captured using BioSpa Live Cell Analysis System (Biotek) and tail moment was calculated using OpenComet70 (link), a plugin for the image processing program ImageJ.
Sun Y., Baechler S.A., Zhang X., Kumar S., Factor V.M., Arakawa Y., Chau C.H., Okamoto K., Parikh A., Walker B., Su Y.P., Chen J., Ting T., Huang S.Y., Beck E., Itkin Z., McKnight C., Xie C., Roper N., Nijhawan D., Figg W.D., Meltzer P.S., Yang J.C., Thomas C.J, & Pommier Y. (2023). Targeting neddylation sensitizes colorectal cancer to topoisomerase I inhibitors by inactivating the DCAF13-CRL4 ubiquitin ligase complex. Nature Communications, 14, 3762.
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5

Quantifying Apoptosis in Tube Assay

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A tube assay for apoptosis assessment was performed in a 96-well plate. Manipulations with Matrigel were performed as described above; 20 × 103 HUVECs and 100 μL of medium per well were used. Incucyte® Caspase-3/7 Green Dye (4440, eBioScience, Ann Arbor, MI, USA) at a final concentration of 5 μM was added to final cell suspensions and was used for apoptosis evaluation. Every 4 h, using 4× objective and the same protocol, the BioSpa Live Cell Analysis System (BioTek, USA) performed panoramic imaging in bright field and “GFP” channel (4 fields of view for each well with their subsequent stitching). The sum area occupied by the cells was calculated from bright field images, and the sum area of activated Incucyte® Caspase-3/7 Green Dye–from GFP channel images. Relative caspase-3 activity was determined by the following formula: caspase-3/7 dye sum area/cells sum area.
Slobodkina E., Boldyreva M., Karagyaur M., Eremichev R., Alexandrushkina N., Balabanyan V., Akopyan Z., Parfyonova Y., Tkachuk V, & Makarevich P. (2020). Therapeutic Angiogenesis by a “Dynamic Duo”: Simultaneous Expression of HGF and VEGF165 by Novel Bicistronic Plasmid Restores Blood Flow in Ischemic Skeletal Muscle. Pharmaceutics, 12(12), 1231.
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