Log In Sign up
The largest database of trusted experimental protocols

35s express35s protein labeling mix

Manufactured by PerkinElmer
Visit Supplier

[35S] EXPRESS35S Protein Labeling Mix is a radioactive solution containing [35S]-methionine and [35S]-cysteine that can be used for metabolic labeling of proteins. It is intended for research use only.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

35S] Protein Labeling Mix (5 citations) EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (2 citations) EasyTag EXPRESS35S Protein Labeling Mix [35S] (2 citations)

3 protocols using 35s express35s protein labeling mix

1

Protein Secretion Analysis in OI Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
OI patients' fibroblasts were plated in 24-well plates and labeled with 5 µCi/ml [35S] EXPRESS35S Protein Labeling Mix (PerkinElmer) in DMEM without L-methionine, L-cystine and L-glutamine for 1 h at 37°C. Total proteins from medium and cell layer were precipitated with 10% trichloroacetic acid. Proteins were washed with acetone twice and resuspended in 60 mM Tris-HCl, pH 6.8, 10% sodium dodecyl sulphate. The radioactivity (CPM) of the samples was measured using a liquid scintillation analyzer (TRI-CARB 2300 TR). The percentage of protein secretion was calculated based on the ratio between the CPM in the media and the CPM in medium and cell layer, evaluated in five technical replicates.
Besio R., Garibaldi N., Leoni L., Cipolla L., Sabbioneda S., Biggiogera M., Mottes M., Aglan M., Otaify G.A., Temtamy S.A., Rossi A, & Forlino A. (2019). Cellular stress due to impairment of collagen prolyl hydroxylation complex is rescued by the chaperone 4-phenylbutyrate. Disease Models & Mechanisms, 12(6), dmm038521.
+ Open protocol
+ Expand
2

Radioactive Protein Labeling in Osteoblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
OBs were labelled with 5 mCi/ml [35S] EXPRESS35S Protein Labeling Mix (PerkinElmer) in α-MEM without L-methionine, L-cystine, and L-glutamine for 1 h at 37 °C. The experiment was performed on three independent OB preparations. Upon protein precipitation, the radioactivity (counts for minute, CPM) of the samples was quantified using a liquid scintillation analyzer (TRI-CARB 2300 TR) (47 (link)).
Joiner W.J., Wang L.Y., Tang M.D, & Kaczmarek L.K. (1997). hSK4, a member of a novel subfamily of calcium-activated potassium channels. Proceedings of the National Academy of Sciences of the United States of America, 94(20), 11013-11018.
+ Open protocol
+ Expand
3

Osteoblast Protein Synthesis Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
OBs were labelled with 5 mCi/ml [35S] EXPRESS35S Protein Labeling Mix (PerkinElmer) in α-MEM without L-methionine, L-cystine, and L-glutamine for 1 h at 37 °C. The experiment was performed on three independent OB preparations. Upon protein precipitation, the radioactivity (counts for minute, CPM) of the samples was quantified using a liquid scintillation analyzer (TRI-CARB 2300 TR) (47) .
Besio R., Contento B.M., Garibaldi N., Filibian M., Sonntag S., Shmerling D., Tonelli F., Biggiogera M., Brini M., Salmaso A., Jovanovic M., Marini J.C., Rossi A, & Forlino A. (2023). CaMKII inhibition due to TRIC-B loss-of-function dysregulates SMAD signaling in osteogenesis imperfecta. Matrix biology : journal of the International Society for Matrix Biology, 120.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!