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Albustix reagent strips

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Albustix reagent strips are a laboratory diagnostic tool used to detect the presence and measure the concentration of albumin in urine samples. These strips provide a quick and convenient way to perform this analysis.

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Albustix (16 citations) Albustix strips (5 citations)

4 protocols using albustix reagent strips

1

Urine Protein Assessment Protocol

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The assessment of proteinuria has been described in detail previously (13 (link)). Urine (10 ml) was manually expressed into a sterile container from each mouse on days 0 and 7 following treatment, and assayed for the presence of protein, specifically albumin, using a colorimetric method with Albustix reagent strips (Bayer Corporation, Elkhart, IN, USA).
Wang X., Zhang Y., Wang Z., Liu X., Zhu G., Han G., Chen G., Hou C., Wang T., Shen B., Li Y., Xiao H., Ma N, & Wang R. (2017). Anti-IL-39 (IL-23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus-like mice. Molecular Medicine Reports, 17(1), 1660-1666.
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2

Adoptive transfer of Tregs in lupus

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BWF1 mice were monitored regularly. The sera IgG and anti-DNA autoantibodies were measured by ELISA. Proteinuria was assessed using Albustix reagent strips (Bayer, Elkart, IN). When the proteinuria developed, BWF1 mice were received with a single dose of 300µg anti-mouse CD3 antibody (17A2, BioLegend) to deplete the endogenous CD3+ T cells (18 ), or the isotype control. 7 days later, CD4med or iTreg were adoptively transferred to the mice with antibody treatment. The percentages of plasma were detected and sera were collected 0, 1, 2 weeks after cell transfer for IgG measurement. While homogeneous iTreg (derived from 10–12 weeks age NZM2328) and nTreg (sorted from the thymus in 10–12 weeks age NZM2328) were adoptively transferred to another lupus-prone mouse, NZM2328 (more than 4 months age), the sera were also collected. Proteinuria and anti-DNA autoantibodies were measured. Sera IgG and IgM autoantibodies cluster was measured by Autoantigen Microarrays.
Xu A., Liu Y., Chen W., Wang J., Xue Y., Huang F., Rong L., Lin J., Liu D., Yan M., Li Q.Z., Li B., Song J., Olsen N, & Zheng S.G. (2016). TGF-β-induced regulatory T cells directly suppress B cell responses through a non-cytotoxic mechanism. Journal of immunology (Baltimore, Md. : 1950), 196(9), 3631-3641.
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3

Pristane-Induced Lupus Model in Mice

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SLE was induced by a single i.p. injection of 0.5 ml pristane to 8-wk-old WT or P2rx7−/− female mice. Mice were monitored for clinical signs of SLE until the end of the study (33 wk after dosing) and examined for lesions consistent with SLE by kidney histology and immunofluorescence, presence of serum autoantibodies by ELISA, and cell subsets composition by flow cytometry. Proteinuria was measured using Bayer Albustix reagent strips on collected fresh spots of urine from each mouse. Score from 0 to 4 was applied as follows: 0, none; 1, trace; 2, 30 mg/ml; 3, 100 mg/ml; and 4, ≥500 mg/ml.
Faliti C.E., Gualtierotti R., Rottoli E., Gerosa M., Perruzza L., Romagnani A., Pellegrini G., De Ponte Conti B., Rossi R.L., Idzko M., Mazza E.M., Bicciato S., Traggiai E., Meroni P.L, & Grassi F. (2019). P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. The Journal of Experimental Medicine, 216(2), 317-336.
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4

Weekly Urine Protein Monitoring

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Urine was manually expressed from each mouse on a weekly basis, collected into a sterile container, and assayed for the presence of protein (specifically albumin) using a colorimetric method (Albustix Reagent Strips, Bayer Corporation, Elkhart, IN).
He Y., Xu R., Zhai B., Fang Y., Hou C., Xing C., Xiao H., Chen G., Wang X., Ma N., Han G, & Wang R. (2020). Hspa13 Promotes Plasma Cell Production and Antibody Secretion. Frontiers in Immunology, 11, 913.
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