Bact alert 3d system

Blood specimens were sent to the microbiology laboratory and cultured by the BacT/ALERT 3D system (Becton-Dickinson, Sparks, MD, USA). Bruker Daltonics Data Analysis was used to identify microbial species. Antimicrobial susceptibility testing was identified by the Vitek 2 automated system (bioMerieux, France) or the Kirby-Bauer Disk Diffusion method (Oxoid, UK). Minimum inhibitory concentrations (MICs) and inhibition zone diameters were interpreted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) [24 ]. An MIC for ertapenem ≥ 1 mg/L or for imipenem or meropenem ≥ 2 mg/L was regarded as carbapenem resistant [24 ]. But tigecycline susceptibilities were judged by the U.S. Food and Drug Administration (FDA) break points, while colistin susceptibilities were determined by the European Committee on Antimicrobial Susceptibility Testing (EU-CAST) criteria [25 (link), 26 ].

This retrospective study was performed in the Chinese People’s Liberation Army General Hospital (CPLAGH). All data were collected by reviewing the prospective real-time nosocomial infection surveillance system (RT-NISS) medical electronic database in the hospital. The system is capable of daily, automatic, and prospective screening of all suspicious infections of hospitalized patients, such as BSI, respiratory tract infection, urinary tract infection, surgical site infection, gastrointestinal tract infection, skin and soft tissue infection and other infections. Simultaneously, the system also can eliminate contamination and colonization infections. A previous study showed that the sensitivity and specificity of the RT-NISS system were 98.8% and 93.0%, respectively, for nosocomial infections [11 ].
Blood was cultured with the BacT/ALERT® 3D™ system (Becton–Dickinson, Sparks, MD, USA) in the microbiology laboratory. Species were identified and then in vitro antibiotic susceptibility was determined with Vitek II (bioMérieux, France).

Blood was cultured using BacT/ALERT 3D system (Becton-Dickinson, Sparks, MD, USA) in the microbiology laboratory. Species identification was performed using the VITEK 2 system (BioMérieux, Marcy 1′Étoile, France). Antibiotic susceptibility testing was performed using the VITEK 2 system or the Kirby-Bauer Disk Diffusion method (Oxoid, UK) according to the recommendations proposed by the Clinical and Laboratory Standards Institute (CLSI).

Blood was cultured with BacT/ALERT 3D system (Becton-Dickinson, Sparks, MD, USA). Microorganism species were identified using the VITEK 2 system (BioMérieux, Marcy 1 Étoile, France). Antibiotic susceptibility testing was determined by the VITEK 2 system or the Kirby-Bauer Disk Diffusion method (Oxford, UK) in accordance with the guideline proposed by the Clinical and Laboratory Standards Institute (CLSI). According to the World Health Organization (WHO) priority list of antibiotic-resistant bacteria, the study included corresponding antibiotic-resistant bacteria responsible for BSI, such as: carbapenem-resistant Acinetobacter baumannii, carbapenem-resistant Pseudomonas aeruginosa [17 (link)].

Blood was cultured using a BacT/ALERT 3D system (Becton-Dickinson, Sparks, MD, USA) in the microbiology laboratory. Species identification was performed using the VITEK 2 system (BioMérieux, Marcy l′Etoile, France). Antibiotic susceptibility testing was performed using the VITEK 2 system or the Kirby-Bauer Disk Diffusion method (Oxoid, UK) according to the recommendations proposed by the Clinical and Laboratory Standards Institute (CLSI) [17 ].

Blood was cultured using a BacT/ALERT 3D system (Becton-Dickinson, Sparks, MD, USA) in the microbiology laboratory. Species identification was performed using Bruker Daltonics DataAnalysis. Antibiotic susceptibility testing was performed using the VITEK 2 (Card number: AST-GN16; AST-GP67) system or the Kirby-Bauer Disk Diffusion method (Oxoid, UK) according to the recommendations proposed by the Clinical and Laboratory Standards Institute (CLSI).