Cd58 ts2 9

Manufactured by BioLegend

CD58 (TS2/9) is a monoclonal antibody that recognizes the human CD58 antigen, also known as LFA-3 (Lymphocyte Function-Associated Antigen 3). CD58 is a cell surface glycoprotein that acts as a ligand for the CD2 receptor, facilitating cell-cell adhesion and communication between various immune cell types.

Automatically generated - may contain errors

Lab products found in correlation

X vivo 15, by Lonza (2 mentions) Hyclone fetal bovine serum, by Thermo Fisher Scientific (1 mentions) Pd 1 eh12.2h7, by BioLegend (1 mentions) Cd28 cd28.2, by Thermo Fisher Scientific (1 mentions) Mopc 21, by BioLegend (1 mentions) Cd40 5c3, by BioLegend (1 mentions)

Spelling variants of this product

TS2/9 (2 citations)

3 protocols using cd58 ts2 9

Detailed T-cell Activation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

RPMI 1640 (31870074), Dynabeads Human T-Activator CD3/CD28 for T cell expansion and Activation kit (1132D) were purchased from Thermofisher Scientific. Hyclone Fetal bovine serum was obtained from Fisher Scientific. Poly-L-lysine solution 0.1% (w/v) in H₂O was obtained from Sigma (25988-63-0). The following anti-human antibodies were purchased from Biolegend, CD62L (DREG-56), CCR7 (G043H7), CD3 (UCHT-1), CD4 (A161A1), CD8 (HIT8a), CD2 (RPA2.10), CD28 (CD28.2), PD-1 (EH12.2H7), CD11a (TS2/4), mouse IgG1 isotype control (MOPC-21), CD58 (TS2/9), mouse IgG1 isotype control (MG1-45; 401402), CD45 (HI30), CD127 (A019D5), HLA-A2 (BB7.2), CD45RO (UCHL1). The following anti-human antibodies were purchased from BD Bioscience, CD45RA (HI100), CD127 (HIL-7R-M21), CD58 (1C3), CD2 (CD2.1) clone was a gift of D. Olive (Aix Marseille University). The following anti-human antibodies were purchased from eBioscience, CD8 (OKT8) and CD28 (CD28.2). Flow cytometry mAbs were used between 1-5ug/ml depending on how many cells were staining (i.e. between 5x10³- 1x10⁶). The following antibodies were purchased from Cell Signaling, pPLCγ1(pY783), pLAT(pY171), pSFK (pY416) (D49G4) and used at dilutions recommended by the company. The following anti-mouse antibodies were purchased from Biolegend, CD4 (RM4- 5), CD3 (145-2C11), TCRβ (H57-97). TCRβ (H57-97) Fab was obtained from Bio X Cell.

Demetriou P., Abu-Shah E., Valvo S., McCuaig S., Mayya V., Kvalvaag A., Starkey T., Korobchevskaya K., Lee L.Y., Friedrich M., Mann E., Kutuzov M.A., Morotti M., Wietek N., Rada H., Yusuf S., Afrose J., Siokis A., Meyer-Hermann M., Ahmed A.A., Depoil D, & Dustin M.L. (2020). A dynamic CD2 rich compartment at the outer edge of the immunological synapse boosts and integrates signals. Nature immunology, 21(10), 1232-1243.

+ Open protocol

+ Expand

Modulating T Cell Activation via Coculture

Check if the same lab product or an alternative is used in the 5 most similar protocols

In some coculture experiments, blocking antibodies (final concentration: 10 µg/mL) against CD54 (HCD54) (BioLegend, San Diego, CA), CD58 (TS2/9) (BioLegend, San Diego, CA), CD40 (5C3) (BioLegend, San Diego, CA), and CD40L (24–31) (BioLegend, San Diego, CA) and an isotype control (T8E5) (InVivoGen, San Diego, CA) were added to KCs cocultured with naive T cells in serum-free medium (XVIVO-15, Lonza, Basel). T cell activation was assessed after 24 h by analyzing the expression of the activation markers CD25 and CD69.

Orlik C., Deibel D., Küblbeck J., Balta E., Ganskih S., Habicht J., Niesler B., Schröder-Braunstein J., Schäkel K., Wabnitz G, & Samstag Y. (2019). Keratinocytes costimulate naive human T cells via CD2: a potential target to prevent the development of proinflammatory Th1 cells in the skin. Cellular and Molecular Immunology, 17(4), 380-394.

+ Open protocol

+ Expand

Blocking T cell adhesion and activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

In some coculture experiments, blocking antibodies (final concentration: 10 µg/mL) against CD6 (M-T605) (BD Bioscience, Franklin Lakes, New Jersey), CD166 (3A6) (Biolegend, San Diego, California), CD318 (CUB1) (Biolegend, San Diego, California), CD58 (TS2/9) (Biolegend, San Diego, California) and isotype control (T8E5) (In vivo Gen, San Diego, California) were added to KCs together with PBTs in serum-free medium (XVIVO-15, Lonza, Basel). T cell activation was assessed after 24 h by analyzing the expression of activation markers CD25 and CD69. T cell adhesion was assessed after 24h, by staining with CD3-APC antibody. Adherent T cells were counted by immune fluorescence microscopy. T cell proliferation was analyzed by destaining of CFDA using flow cytometry after 72 h.

Orlik C., Berschneider K.M., Jahraus B., Niesler B., Balta E., Schäkel K., Schröder-Braunstein J., Souto-Carneiro M.M, & Samstag Y. (2022). Keratinocyte-induced costimulation of human T cells through CD6 - but not CD2 - activates mTOR and prevents oxidative stress. Frontiers in Immunology, 13, 1016112.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!