Polaron sc7640 high resolution sputter coater

Cell measurements (n = 50) were made using the 40×/0.65 objective and an Olympus BX51 compound microscope. Four samples were examined in a JEOL JSM-7600F scanning electron microscope (SEM) at a working distance of 15 to 21 mm and a voltage of 1.2 to 5.0 kV after a preliminary wash in distilled water, followed by dehydration in a series of ethanol solutions of increasing concentration (30, 50, 70, 90 and 100%), air drying on 0.5″ aluminum mounts and sputter coating with gold–palladium using a Polaron SC7640 High Resolution Sputter Coater (Quorum Technologies, Newhaven, UK).

Tc samples were treated with 10 µM ergosterol peroxide for 12 h were fixed to be analyzed using a JEOL JSM-7600F scanning electron microscope (Wilmington, Massachusetts, USA) at a working distance of 15 to 21 mm and a voltage of 1.2 to 5.0 kV after a preliminary wash in distilled water, followed by dehydration in a series of ethanol solutions of increasing concentration (30%, 50%, 70%, 90% and 100%), air drying on 0.5″ aluminum mounts and sputter coating with gold-palladium using a Polaron SC7640 High Resolution Sputter Coater (Quorum Technologies, Newhaven, East Sussex, UK); some micrographs were obtained using a Philips XL-30 environmental SEM (Pl. 7 and 8). Line drawings were made from digital images.