Spd m20a diode array detector dad

The obtained FGFR4/CMCSPs and NCSPs particles were fully characterized by SEM, EDS, TGA and DSC. SEM image was obtained via Hitachi S-4800 field-emission SEM equipped with an EDS detector operating at 15.0 kV (Tokyo, Japan). TGA and DSC results were obtained by STA 449 F3 Jupiter (Selb, Germany). The HPLC/MS system (Shimadzu Corporation, Kyoto, Japan) included a SPD-M20A diode array detector (DAD) and a TOFMS system. Sample volume of 10 μL was analyzed at a flow rate of 1.0 mL min⁻¹ monitoring at 236 nm for PD. The analytical column was a 150 mm × 4.6 mm, 5 mm C₁₈ column (Shimadzu Corporation, Kyoto, Japan). The according mobile phase is consisted of acetonitrile-water-ethanoic acid (70:30:0.5, v/v/v). ASG and its alkaloids were analyzed by 250 mm × 4.6 mm, 5 mm C₁₈ column (Acchrom Corporation, Liaoning, China) monitoring at 220 nm. Mobile phase A: acetonitrile, Phase B: 0.1% aqueous formic acid; gradient: 0–30 min, 5% A–30% A; 30–40 min, 30% A–30%A.

HyE-Ov was subjected to a qualitative chromatographic analysis by High Performance Liquid Chromatography (HPLC) system, equipped with SPD-M20A Diode Array Detector (DAD) (Shimadzu, Japan). The investigations were carried out using a Phenomenex Luna C18(2) column (3 μm x 4.6 mm x 150 mm; 3μm), formic acid 1% (phase A) and methanol (phase B) as solvents and an elution gradient, at flow rate of 0.95 mL/min, set as follows: t0 min (A 85%, B 15%); t20 min (A 65%, B 35%); t55 min (A 10%, B 90%); t68 min (A 85%, B 15%); t70 min (A 85%, B 15%). Chromatograms were acquired by monitoring sample absorbance at 280 nm. Oregano extract fractionation was performed under the same conditions. A total of 8 fractions were separated: 7 of them were collected every 5 minutes of run (from 0 min to 35 min), whereas the eighth one consisted of the final eluate (from 35 min to the end of the run). All fractions were totally concentrated by vacuum dry evaporating (at 30 °C), using a Concentrator Plus (Eppendorf) and stored at -80 °C. For treatments, pellets were suspended in appropriate volumes of RPMI 1640, to obtain the same concentrations present in the original total extract.

The morphology of MUF cubes was observed by scanning electron microscopy (SEM). Images were obtained using a JEOL JSM 6300 microscope at the Central Service for Research Support of the University of Córdoba. Chromatographic analysis was carried out on a Shimadzu (Kyoto, Japan) HPLC system coupled to a SPD-M20A Diode Array Detector (DAD). The column used for the separation was a Hypersil ODS (250 × 4.6 mm, 5 µm particle size) from Thermo Fisher Scientific (Waltham, MA, USA), kept at 25 °C in a CTO 10AS column oven. Samples were injected using a Rheodyne injector with a sample loop of 20 µL volume. The mobile phase consisted of water (A) and acetonitrile (B), containing 0.1% (v/v) formic acid. The analytes were separated following a gradient elution program from 55% to 80% B in 30 min and then to 85% in 5 min. The total chromatogram time was 35 min. Mobile phase was delivered using a LC20AD pump, at a flow rate of 0.8 mL min⁻¹. The detector was set at a wavelength range of 200–360 nm. Data acquisition and processing were carried out using a LC-solution software version 1.21. FTIR spectra were recorded on a Spectrum Two FTIR using an attenuated total reflectance accessory (PerkinElmer, Cambridge, MA, USA). Approximate contact angle measurements were carried out by analyzing various photographs of water droplets on MUF cubes using the Corel Draw X6 software.