Curcumin, adenine, and potassium oxonate were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Antibody against ZO-1, occluding, and claudin-1 were purchased from Abcam (Cambridge, MA, USA).
Curcumin
Manufactured by Solarbio
Sourced in China
Curcumin is a natural compound derived from the turmeric plant. It is a yellow-colored powder with antioxidant and anti-inflammatory properties. Curcumin is commonly used as a research tool in various scientific studies and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Adenine, by Solarbio (1 mentions)
Potassium oxonate, by Solarbio (1 mentions)
Claudin 1, by Abcam (1 mentions)
Phosphate buffer saline pbs ph 7.4, by Solarbio (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Anti β actin antibody, by Thermo Fisher Scientific (1 mentions)
Anti vimentin antibody, by Cell Signaling Technology (1 mentions)
Anti β catenin antibody, by Cell Signaling Technology (1 mentions)
Cck 8 kit, by Solarbio (1 mentions)
Anti e cadherin antibody, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Hrp labeled goat anti rabbit igg, by Abcam (1 mentions)
Anti f actin antibody, by Abcam (1 mentions)
Anti shh, by Abcam (1 mentions)
Hrp labeled goat anti mouse igg, by Abcam (1 mentions)
Anti gli1 antibody, by Abcam (1 mentions)
Anti foxm1 antibody, by Abcam (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc hcl, by Merck Group (1 mentions)
3 4 5 dimethylthiahiazo z y1 3 5 diphenytetrazoliumromide mtt, by Merck Group (1 mentions)
Lecithin, by Solarbio (1 mentions)
14 protocols using curcumin
1
Curcumin, Adenine, and Potassium Oxonate Protocol
2
Curcumin-Loaded Lipid Nanoparticles
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ABZ was obtained as a gift sample from Vetindia Pharmaceuticals Limited (Hyderabad, Andhra Pradesh, India). Curcumin, phosphate buffer saline (PBS, pH 7.4) and glyceryl trimyristate were purchased from Solarbio Life Sciences (Beijing, China). Tween 80 and all solvents used in this study were acquired from Tianjin Chemical and Reagents (Tianjin, China).
3
Curcumin Regulates Gastric Cancer Cell Signaling
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The human gastric cancer cell line, SGC-7901 was obtained from the Laboratory of Pathology, School of Basic Medical, Lanzhou University (Lanzhou, China),31 (link) and the cells were authenticated by STR. Cells were cultured in RPIM-1640 (HyClone, UT, USA) supplemented with 10% fetal bovine serum (FBS; Kibbutz Beit Haemek, Israel) and 1% penicillin/streptomycin (Sigma-Aldrich, MO, USA) in a humidified atmosphere of 5% CO2 at 37°C. Curcumin and a CCK-8 kit were purchased from Beijing Solarbio Science & Technology (Beijing, China).
Primary antibodies included: Anti-Shh (Abcam, Cambridge, UK), anti-Gli1 antibody (Abcam), anti-Foxm1 antibody (Abcam), anti-β-catenin antibody (Cell Signaling Technology, MA, USA), anti-E-Cadherin antibody (Cell Signaling Technology), anti-vimentin antibody (Cell Signaling Technology), anti-F-actin antibody (Abcam) and anti-β-actin antibody (Thermo Fisher Scientific, MA, USA). Secondary antibodies included: HRP-labeled goat anti-rabbit IgG (Abcam) and HRP-labeled goat anti-mouse IgG (Abcam). All the primary antibodies were diluted to 1:1000. The secondary antibodies were diluted to 1:5000.
Primary antibodies included: Anti-Shh (Abcam, Cambridge, UK), anti-Gli1 antibody (Abcam), anti-Foxm1 antibody (Abcam), anti-β-catenin antibody (Cell Signaling Technology, MA, USA), anti-E-Cadherin antibody (Cell Signaling Technology), anti-vimentin antibody (Cell Signaling Technology), anti-F-actin antibody (Abcam) and anti-β-actin antibody (Thermo Fisher Scientific, MA, USA). Secondary antibodies included: HRP-labeled goat anti-rabbit IgG (Abcam) and HRP-labeled goat anti-mouse IgG (Abcam). All the primary antibodies were diluted to 1:1000. The secondary antibodies were diluted to 1:5000.
4
Targeted Delivery of Curcumin Nanoparticles
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Curcumin, lecithin, PBS (pH 7.4), Dulbecco's modified Eagle's medium (DMEM), 0.25% trypsin-EDTA, heat-inactivated FBS, and Dimethyl sulfoxide (DMSO) were purchased from Solarbio Life Sciences (Beijing, China). Chitosan (CS), perfluorohexane (PFH), Tween 20, Sephadex G-50, and dialysis membranes (MWCO: 12,000) were purchased from Muke Biotech (Zhejiang, China). Rhodamine B waspurchased from Sangon Biotech (Shanghai, China). Arg-Gly-Asp-l-Phe-Lys (RGDfk), N-hydroxysulfosuccinimide (sulfo-NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC-HCl), and 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazolium-romide (MTT) were from Sigma-Aldrich (MO, USA). Other chemicals and solvents were obtained commercially of high-performance liquid chromatography (HPLC) or analytical grade. MDA-MB-231 breast cancer cells were from the Cell Bank of the Chinese Academy of Sciences in Shanghai.
5
Curcumin Cytotoxicity in SKCM Cells
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6
Osteogenic Differentiation of BMSCs
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BMSCs were seeded at a density of 50 cells/cm2 and cultured until they reached 70% confluency. The CCM was then changed to OM (fresh complete medium with 15% FBS) supplemented with 50 µg/ml ascorbic acid (Sigma Aldrich; Merck KGaA, Darmstadt, Germany), 10 mM β-glycerol phosphate disodium salt (Sigma Aldrich; Merck KGaA), and 100 nM dexamethasone (Sigma Aldrich; Merck KGaA). Cells cultured in osteogenic medium only comprised the OM group; while in the curcumin group (CR group), 15 µM curcumin was added to the OM. This concentration was selected based on previous studies that used curcumin in cell culture to induce osteogenic differentiation with minimal cytotoxicity (53 (link),78 (link),79 (link)). curcumin was prepared according to the method previously described by Gu et al (53 (link)). Briefly, curcumin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) was dissolved in dimethyl sulfoxide and stored at −20°C. In the ATRA group, 1 µM ATRA (Sigma-Aldrich; Merck KGaA) was added to the OM. This concentration has repeatedly been used to study the effects of ATRA on in vitro osteogenic differentiation; since the normal physiological level of ATRA is ≤0.01 µM and the effective pharmacological concentration is >0.1 µM, the 1 µM concentration was selected to induce osteogenic differentiation (27 (link),80 (link)–82 (link)).
7
Culturing Human Tongue Cancer Cells
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Human tongue cancer cell line (CAL 27) was provided by Procell Life Science & Technology (Wuhan, China) and was cultured in DMEM supplemented with 1 penicillin/streptomycin and 20% FBS at 37°C under one atmosphere with 5% CO2 in humidified air. Curcumin was produced by Solarbio Life Sciences Inc. (Beijing, China).
8
Cell Culture and Compound Preparation Protocol
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Dulbecco’s
modified Eagle’s
medium (DMEM), trypsin with/without ethylenediaminetetraacetic acid
(EDTA), penicillin–streptomycin, and sterile phosphate-buffered
saline (PBS) were obtained from Solarbio Co., Ltd. (Beijing, China).
Reduced GSH, quercetin, rutin, andrographolide, resveratrol, polydatin,
curcumin, and ursolic acid were obtained from Solarbio Co., Ltd. (Beijing,
China), and all of the plant components are standards with purity
greater than 99%. Fetal bovine serum (FBS) was purchased from Gibico
(Gaithersburg, MD). quercetin stock (25 mM) was dissolved in dimethyl
sulfoxide (DMSO) and preserved under −20 °C. Dextran sulfate
sodium salt (DSS, 36 000–50 000 Da) was purchased
from MP Biomedicals.
modified Eagle’s
medium (DMEM), trypsin with/without ethylenediaminetetraacetic acid
(EDTA), penicillin–streptomycin, and sterile phosphate-buffered
saline (PBS) were obtained from Solarbio Co., Ltd. (Beijing, China).
Reduced GSH, quercetin, rutin, andrographolide, resveratrol, polydatin,
curcumin, and ursolic acid were obtained from Solarbio Co., Ltd. (Beijing,
China), and all of the plant components are standards with purity
greater than 99%. Fetal bovine serum (FBS) was purchased from Gibico
(Gaithersburg, MD). quercetin stock (25 mM) was dissolved in dimethyl
sulfoxide (DMSO) and preserved under −20 °C. Dextran sulfate
sodium salt (DSS, 36 000–50 000 Da) was purchased
from MP Biomedicals.
9
Curcumin and Opioid Receptor Modulators
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Curcumin was purchased from Solarbio (Beijing, China) with 95% purity. Naloxone, β-Funaltrexamine (β-FNA), nor-binaltorphimine (nor-BNI), and naltrindole (NTI) were purchased from Sigma-Aldrich (St. Louis, MO, United States). β-endorphin antiserum and enkephalin antiserum were purchased from Abcam (Cambridge, United Kingdom). Curcumin was dissolved in a vehicle with 5% dimethyl sulfoxide (DMSO), 10% Cremophor EL, and 85% saline. Other drugs were dissolved in saline.
10
Curcumin Cytotoxicity Assay Protocol
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The cell concentration was diluted to 3 × 104 cells/ml and seeded in 96-well plates. On the next day, the concentration of dissolved curcumin (Solarbio, Beijing, China) was diluted to 80 μM, 40 μM, 20 μM, and 10 μM, respectively. The medium of the 96-well plate was replaced to the fresh medium containing the above drug concentrations. Another negative control (NC) group was set up. The 96-well plates were placed in a CO2 incubator for 24 h, 48 h, and 72 h. The cells were then added with 5 mg/ml MTT solution (Solarbio, Beijing, China) for 4 h and then dissolved in DMSO to detect the A490 value.
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