Conditioned media was collected from either MDI-GSH conjugate-treated THP-1 macrophages or miR-inhibitor transfected THP-1 macrophages as described above. The following enzyme-linked immunosorbent assay (ELISA) kits were obtained from ThermoFisher Scientific: Human CCL2/MCP-1 (Cat# 88739922), human CCL3/MIP-1α (#88703522), and human IL8/CXCL8 (#88808622). Human CCL5/RANTES ELISA kit (Cat# DY27805) was obtained from R&D systems (Minneapolis, MN). The assay sensitivity for each chemokine is as follows: CCL2 (7 pg/ml), CCL3 (16 pg/ml), CCL5 (15.6 pg/ml), and IL8/CXCL-8 (2 pg/ml). Human CCL2, CCL3, CCL5, and IL8 released into the conditioned media from THP-1 macrophages were measured by ELISA according to the manufacturer’s instructions.
Human ccl5 rantes elisa kit
Manufactured by R&D Systems
Sourced in United Kingdom
The Human CCL5/RANTES ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human CCL5/RANTES levels in cell culture supernatants, serum, and plasma.
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3 protocols using human ccl5 rantes elisa kit
1
Quantifying Chemokine Secretion in Macrophages
2
RANTES Quantification by ELISA
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5P12-RANTES concentrations were also quantified by ELISA using the Human CCL5/RANTES ELISA kit (R&D Systems, UK, cat no. DRN00B). Samples were analyzed as per the manufacturer's instructions and the appropriate dilutions were made to ensure all readings fell within range of the standard curve of the kit (0.002–2 ng/mL). The absorbance was measured at 450 nm (EnSpire™ Multimode Plate Reader, PerkinElmer, USA) with correction at 570 nm to account for plate imperfections. A four-parameter logistic (4-LP) standard curve was generated and sample concentrations were calculated relative to it. Results were subsequently multiplied by the appropriate dilution factor.
3
Measuring RANTES and IL-17A in PD
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