β-cyclodextrin (Acros Organics, Geel, Belgium), poly (vinyl alcohol) Mw = 89,000–98,000 with 99+% hydrolyzed (Sigma-Aldrich, Saint Louis, MO, USA), citric acid monohydrate (RCI labscan, Bangkok, Thailand), cotton cord (Taisonghuad, Bangkok, Thailand), sodium hypophosphite (Acros Organics, Geel, Belgium), crystal violet (PanReac, Barcelona, Spain), methylene blue (CARLO ERBA Reagents S.A.S., Val de Reuil, France), and paraquat dichloride hydrate (Sigma-Aldrich, Saint Louis, MO, USA) were obtained from commercial sources. Ultrapure water was used for all experiments and other chemicals were analytical grade.
β cyclodextrin
Manufactured by Thermo Fisher Scientific
Sourced in Belgium, United States, Thailand
β-cyclodextrin is a cyclic oligosaccharide composed of seven glucopyranose units. It is a white, crystalline, water-soluble powder. β-cyclodextrin is commonly used as a molecular encapsulator in various applications, such as drug delivery, food processing, and chemical synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hypophosphite, by Thermo Fisher Scientific (4 mentions)
Paraquat dichloride hydrate, by Merck Group (4 mentions)
Polyvinyl alcohol (pva), by Merck Group (2 mentions)
Bile salt, by Merck Group (2 mentions)
Maltodextrin de 4 7, by Merck Group (2 mentions)
Quercetin 3 glucoside, by Merck Group (2 mentions)
1 2 3 4 butanetetracarboxylic acid, by Thermo Fisher Scientific (2 mentions)
Crystal violet, by ITW Reagents (1 mentions)
Methylene blue, by Carlo Erba (1 mentions)
Citric acid monohydrate, by RCI Labscan (1 mentions)
Trolox, by Thermo Fisher Scientific (1 mentions)
Porcine pancreatin, by Merck Group (1 mentions)
Gum arabic, by Thermo Fisher Scientific (1 mentions)
Mili q system, by Merck Group (1 mentions)
2 4 6 tri 2 pyridyl s triazine tptz, by Thermo Fisher Scientific (1 mentions)
Porcine gastric mucosa pepsin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Brucella broth, by BD (1 mentions)
Polymyxin b, by Merck Group (1 mentions)
Vancomycin, by Merck Group (1 mentions)
12 protocols using β cyclodextrin
1
Multifunctional Cotton Cord Fabrication
2
Standardized Preparation and Analysis of Plant Extracts
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Mili-Q system (Millipore, Bedford, MA, USA) was applied for the purification of distilled water. Kemika d.d. (Zagreb, Croatia) supplied ethanol (96%), methanol, sodium chloride, Fe (III) chloride hexahydrate, sodium acetate (99%), sodium bicarbonate, and HPLC grade formic acid (99%). Sigma Aldrich (St. Louis, MO, USA) supplied maltodextrin (DE 4–7), bile salts, porcine pancreatin, porcine gastric mucosa pepsin, and 2,2-diphenyl-1-(2,4,6- trinitrophenyl) hydrazyl (DPPH), as well as standards of quercetin-3-glucoside, myricetin, gallic, syringic, ferulic, protocatechuic, caffeic, chlorogenic, p-coumaric, and rosmarinic acid. Gum arabic, β-cyclodextrin, Trolox, and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were obtained from Acros Organics (Geel, Belgium). Honeywell Riedel-de-Haën (Charlotte, NC, USA) supplied fluorescein sodium salt, while J.T.Baker (Deventer, The Netherlands) supplied glacial acetic acid, hydrochloric acid (37%), and HPLC grade acetonitrile. Extrasynthese (Genay, France) supplied standards of procyanidin B2, rutin, kaempferol-3-glucoside, luteolin, apigenin, catechin, epigallocatechin gallate, and epicatechin gallate. Methanol (ethanol with 0.5% v/v DMSO for apigenin) stock solutions of the standards were used to produce five working standard dilutions.
3
Detailed Protocol for Culturing Hp Strain PMSS1
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H. pylori strain PMSS1, which is also called 10700 and which is CagA+ with an active type IV secretion system (Lee et al, 1997 (link); Arnold et al, 2011 (link)), and derivatives were cultured at 37°C with 10% CO2 and 10% O2 in a trigas incubator (MCO-19M; Sanyo). Cells were grown on solid media containing 4% Columbia agar (BD Biosciences), 5% defibrinated horse blood (HemoStat Laboratories) 0.2% β-cyclodextrin (Acros Organics), 10 μg/ml vancomycin (Sigma-Aldrich), 5 μg/ml cefsulodin (Sigma-Aldrich), 2.5 U/ml polymyxin B (Sigma-Aldrich), 5 μg/ml trimethoprim (Sigma-Aldrich), and 8 μg/ml amphotericin B (Sigma-Aldrich). For mouse infections, bacteria grown on horse blood plates were used to inoculate liquid media (BB10) containing 90% (vol/vol) Brucella broth (BD Biosciences) and 10% fetal bovine serum (Gibco), which was cultured shaking at 200 rpm overnight in a MaxQ 2000 orbital shaker (Thermo Fisher Scientific) and grown to an optical density at 600 nm of 0.4–0.6 (mid-log phase), from which an inoculum of ∼5 × 107Hp cells per 100 μl BB10 was prepared. To determine Hp titers in the stomach, harvested tissues were weighed, homogenized, serially diluted, and plated on the solid media described above, with the addition of bacitracin (200 μg/ml; Acros Organics) to prevent growth of the stomach microbiota.
4
Synthesis and Characterization of Parabens and Metal Complexes
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Standard samples of three paraben compounds, namely, propyl paraben (PP), butyl paraben (BP), and benzyl paraben (ArP), 1-butyl-3-methylimidazolium chloride (BMIM-Cl), toluene-2,4-diisocyanate (TDI), and anhydrous dimethyl sulfoxide-d6 (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Iron(ii ) chloride tetrahydrate (FeCl2·4H2O) and iron(iii ) chloride hexahydrate (FeCl3·6H2O) were purchased from R&M Chemicals (Essex, UK), whereas β-cyclodextrin (βCD, 99%) was commercially available and was purchased from Acros (Hungary). Moreover, acetonitrile (ACN), methanol (MeOH) (HPLC grade, 99.7%), acetone (technical grade), and ammonia solution (25%) were supplied by Friendemann Schmidt (Parkwood, Australia), whereas anhydrous N,N-dimethylformamide (DMF) was purchased from Merck (Kenilworth, NJ, USA). Analytical-grade absolute ethanol (denatured, 99.7%) was purchased from J. Kollin Chemicals (Midlothian, UK), whereas deionised (DI) water (18.2 MΩ cm) was provided by a Sartorius Milli-Q system (Aubagne, France).
5
Curcumin Formulation with Cyclodextrins
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Curcumin 97% was purchased from Alfa Aesar, Thermo Fisher Scientific, Heysham, Lancashire, UK. β-cyclodextrin, hydroxypropyl β-cyclodextrin, and hydroxyethyl β-cyclodextrin were purchased from Acros Organics, NJ, USA. Captisol (β-cyclodextrin sulfobutyl ether sodium salt) was a generous gift from CyDex Pharmaceuticals, Lawrence, KS, USA.
6
Evaluating PFOA Toxicity with β-Cyclodextrin
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Perfluorooctanoic acid (PFOA, 96% purity) was purchased from Sigma-Aldrich (St. Louis, MO, USA). β-Cyclodextrin (β-CD, 98% purity) was purchased from Acros Organics (Geel, Belgium). Both chemicals were used without further purification. Stock solutions of PFOA (7.25 mM, i.e., 3000 parts-per-million (ppm)), 1:1 β-CD:PFOA (β-CD: 7.25 mM, PFOA: 7.25 mM), and 2:1 β-CD:PFOA (β-CD: 14.50 mM, PFOA: 7.25 mM) were prepared with deionized water in polypropylene tubes. The solutions were sonicated until dissolution of the solids was achieved. A control solution of deionized water was also stored in a polypropylene tube. Stock solutions were subsequently diluted over a relevant range of concentrations (see below) for assessment of zebrafish embryo toxicity.
7
Characterization of Phytochemicals and Antioxidants
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Distilled water was obtained using the Milli-Q water purification system (Millipore, Bedford, MA, USA). Ethanol (96%), sodium acetate (99%) and iron (III) chloride hexahydrate were purchased from Kemika d.d. (Zagreb, Croatia), methanol and sodium bicarbonate from Gram-mol d.o.o. (Zagreb, Croatia), and sodium chloride from Lach-ner (Neratovice, Czech Republic). Maltodextrin (DE 4–7) was procured from Sigma-Aldrich (St. Louis, MO, USA), and β-cyclodextrin, gum arabic, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were from Acros Organics (Geel, Belgium). Pepsin, pancreatin, bile salts and 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) were obtained from Sigma-Aldrich (St. Louis, MO, USA) and hydrochloric acid (37%) from Carlo Erba Reagents (Val-de-Reuil, France), while acetic acid was purchased from J.T.Baker (Deventer, The Netherlands). Standards for gallic acid, chlorogenic acid, protocatechuic acid, synaptic acid, ferulic acid, quinic acid, caffeic acid, p-coumaric acid, esculetin, quercetin-3-glucoside, kaempferol-3-glucoside, scopoletin and myricetin were obtained from Sigma-Aldrich (St. Louis, MO, USA) and catechin, epigallocatechin gallate, epicatechin gallate, luteolin, naringenin and apigenin were obtained from Extrasynthese (Genay, France).
8
Synthesis and Characterization of Composite Hydrogels
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5-aminosalicylic acid was purchased from Alfa Aesar (Lancashire, UK). Chitosan (MW = 100,000–300,000) and β-Cyclodextrin (MW = 1396 Da) was acquired from Acros organics (Fair Lawn, NJ, USA) and Alfa Aesar (Thermo Fisher Scientific, Ward Hill, MA, USA), respectively. Acrylic acid (MW = 72.06 g/mol) and ammonium persulfate (MW = 228.21) were sourced from Acros (Carlsbad, CA, USA) and Showa (Tokyo, Japan), respectively, whereas ethylene glycol dimethacrylate (MW = 198.22 g/mol) was purchased from Alfa-Aesar (Tewksbury, MA, USA).
9
Synthesis and Characterization of Cyclodextrin-based Hybrid Materials
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β-cyclodextrin (Acros Organics, Geel, Belgium), 1,2,3,4-butanetetracarboxylic acid (Acros Organics, Geel, Belgium), Poly(vinyl alcohol) Mw = 89,000–98,000 with 99+% hydrolyzed (Sigma-Aldrich, Saint Louis, MO, USA), sodium hypophosphite (Acros Organics, Geel, Belgium), paraquat dichloride hydrate (Sigma-Aldrich, Saint Louis, MO, USA), safranin (S.D. Fine Chem Ltd., Mumbai, India), and malachite green (Riedel-de Haën, Seelze, Germany) were obtained from commercial sources. Other chemicals were analytical grade and ultrapure water was employed for all experiments.
10
Multifunctional Textile Finish Synthesis
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Poly (vinyl alcohol) with Mw = 89,000–98,000 g/mol and 99+% hydrolyzed (Sigma-Aldrich, Saint Louis, MO, USA), cotton cord (Taisonghuad, Bangkok, Thailand), β−cyclodextrin (Acros Organics, Geel, Belgium), 1,2,3,4-butanetetracarboxylic acid (Acros Organics, Geel, Belgium), sodium hypophosphite (Acros Organics, Geel, Belgium), and paraquat dichloride hydrate (Sigma-Aldrich, Saint Louis, MO, USA) were obtained from merchandise sources. Other analytical grade chemicals and ultrapure water were used for all experiments.
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