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7 protocols using sodium benzoate

1

Synthesis of Aluminum-Based MOF Catalysts

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All the reagents and solvents were purchased commercially and were used without further purification. Aluminum isopropoxide (Al(OiPr)3) and methylamine ethanol solution (40%, 120 µL) were acquired from Aladdin Chemical Reagent Shanghai. N‐propyl alcohol (HOnPr), N, N‐dimethylformamide (DMF), and sodium benzoate was bought from Sinopharm Chemical Reagent Beijing. Isonicotinic acid (HIN), N‐methyldiethanolamine (H2mdea) were purchased from Adamas‐beta. 4‐(4‐pyridy) benzoic acid (Hpyba) was acquired from Jilin Chinese Academy of Sciences‐Yanshen Technology Co., Ltd.
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2

BSA Solution Preparation and Characterization

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All chemicals used in this experiment, including BSA (lyophilized powder, Mw ≈ 66.2 kDa, Sigma) and sodium benzoate (Sinopharm), are of high purity. Thus, no further purification is required after purchase. PBS buffer solution (20 mM, pH = 7.40) was prepared with the ultrapure water (ρ = 18.2 MΩ cm). Then, BSA solution was prepared using PBS as a solvent for the spectral experiments. Additionally, all solutions were stored in the dark environment with a low temperature of 4 °C before using.
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3

Chitosan-based Biosensor Development

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Chitosan (food grade, DAC degree: 86%, viscosity: 296 mPa s) was supplied by Zhejiang Golden-Shell Pharmaceutical Co., Ltd. Glutaraldehyde (25%), trichloroethylene and glucose oxidase (from Aspergillus niger, 100 U mg−1) were supplied by Shanghai Macklin Biochemical Co., Ltd. Ammonium ferrous sulfate dodecahydrate (NH4Fe(SO4)2·12H2O), ammonium ferrous sulfate hexahydrate ((NH4)2Fe(SO4)2·6H2O), glucose, 4-hydroxybenzoic acid, hydrogen peroxide (30% w/w), ammonium hydroxide (25%), sodium benzoate and other chemicals were purchased from Sinopharm Chemical Reagent Co., Ltd, China. All chemicals used were of analytical grade or above.
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4

Nicotine Purification and Enzyme Acquisition

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L-Nicotine (purity, ≥99%) was purchased from Fluka Chemie GmbH (Buchs Corp., Switzerland). Restriction enzymes, NcoI and XhoI were purchased from TaKaRa (Dalian, China). Sodium benzoate and glycerin were purchased from Sinopharm Chemical Reagent Co., Ltd. Nucleotide sequencing service was provided by BioSune Company (Shanghai, China).
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5

Synthesis and Characterization of Iron-based Compounds

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Ferrous sulfate heptahydrate (FeSO4·7H2O, 99.9%), trisodium citrate dihydrate (C6H5Na3O7·2H2O, 99%), sodium benzoate (99.5%), p-hydroxybenzoic acid (p-HBA, 99%) and boric acid were purchased from Sinopharm Chemical Regent Co. Ltd., China. Piperazine-N, N-bis-(ethanesulfonic acid) sodium salt (PIPES, 99%) and 2-(N-morpholino)ethanesulfonic acid (MES, 99%) were acquired from Sigma-Aldrich. All other chemicals were of analytical grade or above. Deionized water (18.2 MΩ·cm) produced by a Heal Force NW ultrapure water System was used in all the experiments.
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6

Lipid Encapsulation of Whey and Soy Proteins

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Food-grade TPSO was supplied by Heze Ruipu Peony Industry Technology Development Co., Ltd. (Heze, Shandong, China). The composition of major fatty acids and beneficial components in TPSO was shown in Table S1. Whey protein isolate (WPI, food grade) was purchased from Zhengzhou Weifeng Biotechnology Co., Ltd. (Zhengzhou, Henan, China). Soybean protein isolate (SPI, biological reagent grade), mucin (porcine stomach, biological reagent grade), pepsin (porcine gastric mucosa, 30,000 U/g), bile salt (pig, cholic acid content ≥ 60%), and pancreatin (pancreatic lipase activity ≥ 4000 U/g) were supplied by Yuanye Biological Technology Co., Ltd. (Shanghai, China). Nile red was purchased from Shanghai Macklinn Biochemical Technology Co., Ltd. (Shanghai, China). Gelatin (GE, chemical pure grade), sodium alginate (SA, chemical pure grade), gum arabic (GA, biological reagent grade), sodium carboxymethyl cellulose (CMC-Na, chemical pure grade), sodium benzoate, and other reagents of analytical grade were obtained from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China).
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7

Optimized Avermectin B2 Formulations

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Avermectin B2 technical material (TC, 95%, w/w), EC (5%, w/w), emulsion in water (EW, 2.5%, w/w) and water dispersible granule (WDG, 3.5%, w/w) were provided by Hebei Xingbai Agricultural Technology Co., Ltd. (Hebei, China). Sodium benzoate, α-lactose, glucose and sucrose were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Styryl phenol polyoxyethylene ether (emulsifier 600) and alkylphenol formaldehyde resin polyoxyethylene ether (emulsifier 700) were provided by Cangzhou Hongyuan Agrochemical Co., Ltd. (Hebei, China). Ethyl acetate, chromatographic grade methanol and acetonitrile were bought from J&K Scientific Ltd. (Beijing, China). Parafilm was purchased from Sigma-Aldrich Shanghai Trading Co., Ltd. (Shanghai, China). All the chemicals were used as received. Milli-Q water (18.2 MΩ.cm, TOC ≤ 4 ppb) was used in all analytical experiments.
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