Anti calreticulin

Manufactured by Santa Cruz Biotechnology

Anti-calreticulin is a laboratory reagent used for the detection and quantification of the calreticulin protein in various biological samples. Calreticulin is a calcium-binding protein involved in numerous cellular processes. The anti-calreticulin product can be utilized in various research applications that require the specific identification and measurement of calreticulin.

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Calreticulin (8 citations)

2 protocols using anti calreticulin

Immunoblotting Antibody Panel Validation

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The following antibodies were used for immunoblotting. Abcam: anti-PMP70 (1:1,000 dilution; ab3421), anti-FASN (1μg/ml dilution; ab22759); Cell Signaling: anti-cytochrome oxidase IV (1:250 dilution; 4850), anti-catalase (1:800 dilution; 12980), anti-centromere protein A (1:400 dilution; 2186), anti-calreticulin (1:200 dilution; 12238), anti-FASN (1:1,000 dilution; 3180); Santa Cruz Biotechnology: anti-C13orf31 (1:200 dilution; sc-374553; E7 and 1:500 dilution; sc-376231; E12), anti-caspase 1 p20 (1:250 dilution; sc-1218-R); R&D Systems: anti-IL-1β (1:250 dilution; AF-401-NA); Sigma: anti-β-actin (1:10,000 dilution; A5060). All antibodies used have validation profiles on either Antibodypedia or 1DegreeBio.
The following reagents were used: M-CSF (Peprotech, 300-25), LPS (from Escherichia coli K12, InvivoGen, tlrl-peklps), human IFN-γ (Peprotech, 300-02), murine IFN-γ (Peprotech, 315-05), human IL-4 (Peprotech, 200-04), murine IL-4 (Peprotech, 214-14), ATP (Sigma Aldrich, A2383), C75 (Sigma Aldrich, C5490), etomoxir (Sigma Aldrich, E1905), MitoTEMPO (Sigma Aldrich, SML0737), oligomycin (Sigma Aldrich, O4876), FCCP (Sigma Aldrich, C2920), rotenone (Sigma Aldrich, R8875), antimycin A (Sigma Aldrich, A8674), palmitate-BSA (Seahorse Bioscience, 102720-100), zymosan A (Sigma Aldrich, Z4250), PMA (Sigma Aldrich, P1585), HRP (Sigma Aldrich, P8375), luminol (Sigma Aldrich, A8511).

Cader M.Z., Boroviak K., Zhang Q., Assadi G., Kempster S.L., Sewell G.W., Saveljeva S., Ashcroft J.W., Clare S., Mukhopadhyay S., Brown K.P., Tschurtschenthaler M., Raine T., Doe B., Chilvers E.R., Griffin J.L., Kaneider N.C., Floto R.A., D’Amato M., Bradley A., Wakelam M.J., Dougan G, & Kaser A. (2016). C13orf31 (FAMIN) is a central regulator of immunometabolic function. Nature immunology, 17(9), 1046-1056.

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Comprehensive Immunomodulatory Assay Protocol

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Thymopentin (TP5) was purchased from Meilunbio. Doxorubicin hydrochloride (DOX) was obtained from Aladdin. Fetal bovine serum albumin (FBS) was purchased from Biowest. Dulbecco's modified Eagle's medium (DMEM) was purchased from Gibco. All assay kits, if not specified, were acquired from Beyotime. The antibodies used were as follows: anti-calreticulin, HMGB1, and CD8 were purchased from Santa Cruz Biotechnology; Ki67 was purchased from Bioss; anti-mouse antibodies (FITC-labeled CD3, FITC-labeled CD11c, FITC-labeled CD25, PE-labeled CD8a, PE-labeled CD86, APC-labeled CD4, APC-labeled CD80, Alexa Flour 647-labeled Foxp3) were all purchased from BioLegend. D-Luciferin, Potassium Salt was purchased from Yeasen.

Ding N., He K., Tian H., Li L., Li Q., Lu S., Ding K., Liu J., Nice E.C., Zhang W., Huang C., Tang Y, & Shen Z. (2023). Carrier-free delivery of thymopentin-regulated injectable nanogels via an enhanced cancer immunity cycle against melanoma metastasis. Materials Today Bio, 20, 100645.

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