The permeant used was resorcinol (Sigma Aldrich, Saint-Quentin-Fallavier, France). Phosphate buffered saline (PBS) was obtained from Hyclone laboratories, USA. For the HPLC assays, the additional chemicals used were methanol, ethanol and phosphoric acid (Thermo Fisher Scientific, Illkirch-Graffenstaden, France). Ultra-pure water was obtained directly in the laboratory from a Millipore MilliQ system (Merck Millipore, Molsheim, France).
Millipore milli q system
Manufactured by Merck Group
Sourced in United States, Germany, France, Italy
The Millipore Milli-Q system is a water purification system designed to produce high-quality, ultrapure water for laboratory applications. It employs a multi-stage purification process to remove various contaminants, including organic compounds, ions, and microorganisms, from the input water source.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (9 mentions)
Acetonitrile, by Thermo Fisher Scientific (8 mentions)
Methanol, by Merck Group (6 mentions)
Catechin, by Merck Group (5 mentions)
Formic acid, by Merck Group (5 mentions)
Acetonitrile, by Merck Group (5 mentions)
Quercetin 3 o glucoside, by Extrasynthese (4 mentions)
E piceid, by Merck Group (4 mentions)
E ε viniferin, by Extrasynthese (4 mentions)
Gallic acid, by Merck Group (4 mentions)
Epicatechin, by Merck Group (4 mentions)
Sodium chloride (nacl), by Merck Group (4 mentions)
Ethanol, by Merck Group (4 mentions)
Diethyl ether, by Merck Group (4 mentions)
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Coutaric acid, by Merck Group (3 mentions)
Caftaric acid, by Biosynth (3 mentions)
Procyanidin b1, by Extrasynthese (3 mentions)
Procyanidin b3, by Extrasynthese (3 mentions)
L tryptophan, by Merck Group (3 mentions)
55 protocols using millipore milli q system
1
Resorcinol Permeation Assay Protocol
2
Sodium Alginate-Based Biomaterial Synthesis
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Sodium alginate (Protonal®RF6650, FMC Corporation, Philadelphia, Pennsylvania, USA) was donated by FMC (Campinas, São Paulo, Brazil). Silver nitrate was purchased from Química Contemporânea (Indaiatuba, São Paulo, Brazil). Bovine gelatin was obtained from Gelnex (Itá, Santa Catarina, Brazil). Other chemicals of analytical grade were obtained from Sigma-Aldrich (Darmstadt, Germany). Water from Millipore Milli®-Q system (Merck KGaA, Darmstadt, Germany), home supplied, was used for the preparation of the aqueous solutions.
3
Electrochemical Activation of Screen-Printed Gold Electrodes
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Screen-printed gold electrodes (SPE) (DRP-220AT SPE) were purchased from Metrohm DropSens (Oviedo, Spain). The SPE consisted of a gold working electrode (4 mm in diameter), a gold counter electrode, and a silver pseudo-reference electrode and was made of high-temperature curing inks. The SPE was connected via a cable (CAC, Metrohm DropSens, Oviedo, Spain) to a potentiostat (VSP-300 with ultra-low current cable, BioLogic, Seyssinet-Pariset, France). All measurements were conducted at room temperature in an air-conditioned laboratory.
Before the start of a biosensing experiment, the SPE was mounted in the 3D-printed static cell with the top part enclosing all electrodes, cleaned, and activated according to the procedure described and optimized by Henihan et al. [49 (link)]. In brief, 100 µL of 0.1 mol L−1 sulfuric acid (H2SO4) was applied on all electrodes of the SPE. Then, 10 electrochemical cyclic voltammetry (CV) cycles with a voltage between 0 and 1.6 V, followed by three CV cycles with a voltage between 0 and 1.3 V, were run. After a biosensing experiment was done, the SPE was washed thoroughly with deionized nuclease-free water (Millipore Milli-Q® system, Merck KGaA, Darmstadt, Germany) and cleaned with the same CV procedure.
Before the start of a biosensing experiment, the SPE was mounted in the 3D-printed static cell with the top part enclosing all electrodes, cleaned, and activated according to the procedure described and optimized by Henihan et al. [49 (link)]. In brief, 100 µL of 0.1 mol L−1 sulfuric acid (H2SO4) was applied on all electrodes of the SPE. Then, 10 electrochemical cyclic voltammetry (CV) cycles with a voltage between 0 and 1.6 V, followed by three CV cycles with a voltage between 0 and 1.3 V, were run. After a biosensing experiment was done, the SPE was washed thoroughly with deionized nuclease-free water (Millipore Milli-Q® system, Merck KGaA, Darmstadt, Germany) and cleaned with the same CV procedure.
4
Cultivation of Plant In Vitro Cultures
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Reagents needed for cultivation of in vitro cultures, e.g., MS and SH basal media, agar, PGRs, and sucrose were acquired from Duchefa Farma B.V. (Haarlem, The Netherlands).
Ultrapure water was obtained using Millipore Milli-Q system (Merck Millipore, Molsheim, France). Acetonitrile and methanol were purchased from Thermo Fisher Scientific (Illkirch-Graffenstaden, France). Standard compounds L-leucine, L-isoleucine, L-phenylalanine, L-tyrosine, L-tryptophan, L-proline, citric acid, gallic acid, coutaric acid, trans-resveratrol, cis-resveratrol, E-piceid, catechin, epicatechin, catechin gallate, tyrosinase, and L-DOPA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rhamnetin, E-ε-viniferin, quercetin 3-O-glucoside, quercetin 3-O-glucuronide, quercetin-3-O-galactoside, procyanidin B1, procyanidin B2, procyanidin B3, and procyanidin C1 were obtained from ExtraSynthèse (Genay, France); caftaric acid was delivered by Carbosynth (Compton, Berkshire, UK), while E-ε-viniferin, Z/E-vitisin B, ampelopsin A, and hopeaphenol were obtained by previous extraction from grape stems [50 (link)].
Ultrapure water was obtained using Millipore Milli-Q system (Merck Millipore, Molsheim, France). Acetonitrile and methanol were purchased from Thermo Fisher Scientific (Illkirch-Graffenstaden, France). Standard compounds L-leucine, L-isoleucine, L-phenylalanine, L-tyrosine, L-tryptophan, L-proline, citric acid, gallic acid, coutaric acid, trans-resveratrol, cis-resveratrol, E-piceid, catechin, epicatechin, catechin gallate, tyrosinase, and L-DOPA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rhamnetin, E-ε-viniferin, quercetin 3-O-glucoside, quercetin 3-O-glucuronide, quercetin-3-O-galactoside, procyanidin B1, procyanidin B2, procyanidin B3, and procyanidin C1 were obtained from ExtraSynthèse (Genay, France); caftaric acid was delivered by Carbosynth (Compton, Berkshire, UK), while E-ε-viniferin, Z/E-vitisin B, ampelopsin A, and hopeaphenol were obtained by previous extraction from grape stems [50 (link)].
5
Cultivation of Plant In Vitro Cultures
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Reagents needed for cultivation of in vitro cultures, e.g., MS and SH basal media, agar, PGRs, and sucrose were acquired from Duchefa Farma B.V. (Haarlem, The Netherlands).
Ultrapure water was obtained using Millipore Milli-Q system (Merck Millipore, Molsheim, France). Acetonitrile and methanol were purchased from Thermo Fisher Scientific (Illkirch-Graffenstaden, France). Standard compounds L-leucine, L-isoleucine, L-phenylalanine, L-tyrosine, L-tryptophan, L-proline, citric acid, gallic acid, coutaric acid, trans-resveratrol, cis-resveratrol, E-piceid, catechin, epicatechin, catechin gallate, tyrosinase, and L-DOPA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rhamnetin, E-ε-viniferin, quercetin 3-O-glucoside, quercetin 3-O-glucuronide, quercetin-3-O-galactoside, procyanidin B1, procyanidin B2, procyanidin B3, and procyanidin C1 were obtained from ExtraSynthèse (Genay, France); caftaric acid was delivered by Carbosynth (Compton, Berkshire, UK), while E-ε-viniferin, Z/E-vitisin B, ampelopsin A, and hopeaphenol were obtained by previous extraction from grape stems [50 (link)].
Ultrapure water was obtained using Millipore Milli-Q system (Merck Millipore, Molsheim, France). Acetonitrile and methanol were purchased from Thermo Fisher Scientific (Illkirch-Graffenstaden, France). Standard compounds L-leucine, L-isoleucine, L-phenylalanine, L-tyrosine, L-tryptophan, L-proline, citric acid, gallic acid, coutaric acid, trans-resveratrol, cis-resveratrol, E-piceid, catechin, epicatechin, catechin gallate, tyrosinase, and L-DOPA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rhamnetin, E-ε-viniferin, quercetin 3-O-glucoside, quercetin 3-O-glucuronide, quercetin-3-O-galactoside, procyanidin B1, procyanidin B2, procyanidin B3, and procyanidin C1 were obtained from ExtraSynthèse (Genay, France); caftaric acid was delivered by Carbosynth (Compton, Berkshire, UK), while E-ε-viniferin, Z/E-vitisin B, ampelopsin A, and hopeaphenol were obtained by previous extraction from grape stems [50 (link)].
6
Preparation of Defined Buffer Solutions
7
Characterization of Phenolic Compounds
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Acetonitrile and methanol were purchased from Thermo Fisher Scientific (France). A Millipore Milli-Q system (Merck Millipore, Molsheim, France) provided ultrapure water. L-leucine, L-isoleucine, L-phenylalanine, L-tyrosine, L-tryptophan, L-proline, citric acid, E-resveratrol, E-piceatannol, catechin, epicatechin, coutaric acid, fertaric acid, E-piceid, kaempferol 3-O-glucoside, and kaempferol 3-O-glucuronide were obtained from Sigma-Aldrich (St. Louis, MO, USA). Caftaric acid was purchased from Carbosynth (Compton, Berkshire, UK). Rhamnetin, E-ε-viniferin, kaempferol 3-O-rutinoside, quercetin 3-O-glucoside, quercetin 3-O-glucuronide, myricetin 3-O-glucoside, procyanidin B1, procyanidin B2, procyanidin B3, procyanidin C1, and kaempferol 3-O-rutinoside were purchased from Extrasynthèse (Genay, France). Pallidol and E-δ-viniferin were graciously provided by Dr. Mary-Lorène Goddard (EA-3991, Université de Haute-Alsace, Colmar, France). E-miyabenol C was obtained from Ambinter (Paris, France).
8
Comprehensive Phytochemical Characterization
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The following reagents were employed: acetone was acquired from Labsynth (Diadema, SP, Brazil), hexane, ethanol, methanol and methyl tert-butyl ether (MTBE) were acquired from Merck (Darmstadt, Germany), vanillin and sulfuric acid (H2SO4) were obtained from Dinâmica Química Contemporânea (Diadema, SP, Brazil), and the Folin–Ciocalteu reagent, sodium carbonate Na₂CO₃, dithiothreitol (DTT), metaphosphoric acid, potassium chloride (KCl), Tris (hydroxymethyl) aminomethane, potassium peroxydisulfate, 2,20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), monobasic and dibasic potassium phosphate, fluorescein sodium salt, 2,20-azobis(2-methylpropionamidine) dihydrochloride (AAPH), sodium hypochlorite solution (NaOCl) and rhodamine 123 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid, ascorbic acid, lutein, zeaxanthin, β-cryptoxanthin, α-carotene β-carotene, catechin, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-car- boxylic acid (Trolox) were used as standards and purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was obtained from a Millipore Milli-Q System (Merck Millipore, Steinfurt, Germany).
9
Raphanus sativus L. Seed Extraction
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Sodium alginate was purchased from DAEJUNG Korea. The seeds of a Raphanus sativus L. plant were purchased from a local store. Absolute alcohol was obtained from Sigma Aldrich with a purity level of 99.9%. Sodium hydroxide (NaOH) and acetic acid (CH3COOH) and NaCl were obtained from Sigma Aldrich (St. Louis, MI, USA). Water from a Millipore Milli®-Q system (Merck KGaA, Darmstadt, Germany) was utilized for the synthesis of the gels and solutions.
10
Antioxidant Potential of Grape Phenolics
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Acetonitrile and ethanol were purchased from Thermo Fisher Scientific (Courtaboeuf, France). A Millipore Milli-Q system (Merck Millipore, Molsheim, Germany) provided ultrapure water. Gallic acid, caffeic acid, E-Resveratrol, E-piceatannol, catechin, epicatechin, gallocatechin, E-piceid, astilbin, quercetin-3-O-glucuronide, procyanidin B1 and procyanidin B2 were obtained from Sigma-Aldrich (St. Louis, MO, USA); E-ε-viniferin and quercetin-3-O-glucoside were purchased from Extrasynthèse (Genay, France) and E-miyabenol C from Ambinter (Paris, France). Pallidol and E-δ-viniferin were graciously provided by Mary-Lorène Goddard (EA-3991, Université de Haute-Alsace, Colmar, France). Z/E-vitisin B, ampelopsin A and hopeaphenol were purified from grape canes as previously described [37 (link)]. The 2,2′-Azo-bis-(2-amidinopropane) hydrochloride (ABAP), fluorescein, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), potassium persulfate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tri(2-pyridyl)-s-triazine (TPTZ), Iron(III) chloride (FeCl3), copper(II) chloride (CuCl2), 2,9-Dimethyl-1,10-phenanthroline (neocuproine) and 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine) were obtained from (Sigma-Aldrich, Saint-Quentin Fallavier, France).
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