E cadherin cdh1

Manufactured by Cell Signaling Technology

Sourced in Germany, United States

E-cadherin/CDH1 is a lab equipment product manufactured by Cell Signaling Technology. It is a protein involved in cell-cell adhesion and plays a key role in the maintenance of tissue architecture and morphogenesis.

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Lab products found in correlation

Axioskop 2 plus microscope, by Zeiss (2 mentions) Axiovision 4, by Zeiss (2 mentions) Synaptophysin syp, by Abcam (2 mentions) Axiocam hrc digital camera, by Zeiss (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Nanodrop, by Thermo Fisher Scientific (2 mentions) Biotinylated secondary antibody, by Agilent Technologies (1 mentions) Ab23375, by Abcam (1 mentions) Ab6556, by Abcam (1 mentions) Fgfr1, by Cell Signaling Technology (1 mentions) Ab5603, by Merck Group (1 mentions) Aldh1a1, by Abcam (1 mentions) Phospho histone h3 p hh3, by Merck Group (1 mentions) Phospho 4ebp1, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Ascl1, by BD (1 mentions) P21 waf1 cip1, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Snai1, by Cell Signaling Technology (1 mentions) Amersham ecl western blotting substrate, by GE Healthcare (1 mentions)

5 protocols using e cadherin cdh1

Histological and Immunohistochemical Analysis of Lung Tissues

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For histological analysis, lungs were inflated and fixed for 24 h with ethanol–acetic acid–formalin (EAF). Fixed tissues were subsequently dehydrated, embedded in paraffin and sections of 2-4 μm were prepared, and stained with hematoxylin and eosin (H&E) for subsequent histopathological analyses. For IHC, tissue sections were rehydrated, blocked in BSA containing PBS, and sequentially incubated with specific primary antibodies and with biotinylated secondary antibodies (DAKO).
The following primary antibodies were applied: CGRP (Sigma, C8198),
E-cadherin/CDH1 (Cell signaling, 3195), FGFR1 (Cell signaling, 9740), GFP (Abcam, ab6556), ALDH1A1 (Abcam, ab23375), EGFR (Abcam, ab52894), SOX2 (Millipore, AB5603), SOX9 (Millipore AB5535), TTF1 (Agilent M357501-2), Synaptophysin/SYP (Abcam, ab32127).
The sections were reviewed with a Zeiss Axioskop2 Plus microscope (Carl Zeiss Microscopy, Jena, Germany) and images were captured with a Zeiss AxioCam HRc digital camera and processed with AxioVision 4 software (both from Carl Zeiss Vision, München, Germany).

Ferone G., Song J.Y., Krijgsman O., van der Vliet J., Cozijnsen M., Semenova E.A., Adams D.J., Peeper D, & Berns A. (2020). FGFR1 Oncogenic Activation Reveals an Alternative Cell of Origin of SCLC in Rb1/p53 Mice. Cell Reports, 30(11), 3837-3850.e3.

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Histological Analysis of Organs

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Animals were sacrificed when they acquired respiratory distress. Tissues and organs were collected and fixed in EAF fixative (ethanol/acetic acid/formaldehyde/saline at 40:5:10:45 v/v) and embedded in paraffin. Sections were prepared at 2 μm thickness from the paraffin blocks and stained with hematoxylin and eosin (HE) according to standard procedures. For immunohistochemistry (IHC), 4 μm-thick sections were made on which the following antibodies were applied: Synaptophysin/SYP (Abcam, ab32127), E-cadherin/CDH1 (Cell Signaling Technology, 3195), NFIB (Thermo Fisher Scientific, PA5-28299), CGRP (Sigma-Aldrich, C8198), Ki67 (Abcam, ab15580), keratin wide-spectrum/KWS (Agilent, Z0622), phospho-histone H3/p-HH3 (Millipore, 04-746), Podoplanin/PDPN (Abcam, ab11936), ASCL1 (BD Biosciences, 556604), ALDH1A1 (Abcam, ab23375), NEUROD1 (Proteintech, 12081-1-ap), phospho-AKT (Cell Signaling Technology, 4060) and phospho-4EBP1 (Cell Signaling Technology, 2855). The sections were reviewed with a Zeiss Axioskop2 Plus microscope (Carl Zeiss Microscopy) and images were captured with a Zeiss AxioCam HRc digital camera and processed with AxioVision 4 software (both from Carl Zeiss Vision).

Böttger F., Semenova E.A., Song J.Y., Ferone G., van der Vliet J., Cozijnsen M., Bhaskaran R., Bombardelli L., Piersma S.R., Pham T.V., Jimenez C.R, & Berns A. (2019). Tumor Heterogeneity Underlies Differential Cisplatin Sensitivity in Mouse Models of Small-Cell Lung Cancer. Cell Reports, 27(11), 3345-3358.e4.

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Western Blot Analysis of Protein Expression

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For total protein extraction, cells and tissue samples were washed with cold PBS prior to lysis with Laemmli Lysis Buffer (50 mM Tris–HCl pH 6.8, 2% SDS, 50 mM NaF, 10 mM β-glycerophosphate, 0.5 mM Na₃VO₄ and 1 × EDTA free protease inhibitors (Roche)) and then the cell lysates were normalized by NanoDrop (Thermo Scientific). Western blotting was carried out as previously described [26 (link)]. Briefly, samples were loaded on 8–10% acrylamide/bis-acrylamide gels and then transferred onto PVDF membrane (Millipore) before immunoblotting with the appropriate primary antibodies overnight at 4 °C. Primary antibodies were used at the following concentrations; AR (dilution 1:000, Cell Signaling #5153), PSA (dilution 1:1000, Cell Signaling #2475), CDC6 (dilution 1:250, Santa Cruz #9964), p21^WAF1/Cip1 (dilution 1:500, Cell Signaling #2947), E-Cadherin (CDH1; dilution 1:500, Cell Signaling #3195) and GAPDH (dilution 1:2000, Cell Signaling #5174). Anti-mouse (Cell Signaling #7076) and anti-rabbit (Cell Signaling #7074) HRP-linked secondary antibodies were used at a dilution of 1:1000.

Mourkioti I., Polyzou A., Veroutis D., Theocharous G., Lagopati N., Gentile E., Stravokefalou V., Thanos D.F., Havaki S., Kletsas D., Panaretakis T., Logothetis C.J., Stellas D., Petty R., Blandino G., Papaspyropoulos A, & Gorgoulis V.G. (2023). A GATA2-CDC6 axis modulates androgen receptor blockade-induced senescence in prostate cancer. Journal of Experimental & Clinical Cancer Research : CR, 42, 187.

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Western Blot Analysis of Epithelial-Mesenchymal Transition

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Protein lysates were harvested from the cells using 1× RIPA lysis buffer (Nacalai Tesque, Kyoto, Japan) and kept on ice for 30 min before being centrifuged at 10,000 g for 10 min at 4 °C to obtain the supernatant. Protein lysates (50 μg) were subjected to SDS-polyacrylamide gel electrophoresis and western blot analysis as described [19 (link)]. Dilutions and the sources of the antibodies used are as follows: E-cadherin (CDH1, 1:1000, Cell Signaling Technology), occludin (OCLN, 1:1000, Merck Millipore), vimentin (VIM, 1:1000, Cell Signaling), SNAI1 (1:250, Cell Signaling) and GAPDH (1:2000, Cell Signaling). The protein bands were detected using a horseradish peroxidase-conjugated secondary antibody (1: 10,000, Abcam, Cambridge, UK) for 1 h at room temperature and visualized with the Amersham ECL Western blotting substrate (GE Healthcare), according to the manufacturer’s protocol. The mean of multiple blots is presented (Fig. 4b); the error bars represent standard errors of the mean, SEM.

Hiew M.S., Cheng H.P., Huang C.J., Chong K.Y., Cheong S.K., Choo K.B, & Kamarul T. (2018). Incomplete cellular reprogramming of colorectal cancer cells elicits an epithelial/mesenchymal hybrid phenotype. Journal of Biomedical Science, 25, 57.

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Molecular Mechanisms of EMT Regulation

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Primers were synthesized by Sangon Biotech Co. (Shanghai, China). Antibodies against GLI1, GLI2, Snail, Slug, E-cadherin (CDH1), vimentin, α-SMA, CK19, and secondary antibodies were obtained from Cell Signaling Technology, Inc. (Beverly, MA, United States). BALB/c mice, 5 weeks old, were purchased from Dossy Experimental Animals Co. (Chengdu, China). HEK293 cells (human embryo kidney cells) were obtained from the American ATCC Cell Line Center.

Siyu P., Junxiang W., Qi W., Yimao Z, & Shuguang J. (2022). The Role of GLI in the Regulation of Hepatic Epithelial–Mesenchymal Transition in Biliary Atresia. Frontiers in Pediatrics, 10, 861826.

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