Fluoview fv10 asw 4.0 viewer software

The CDS of PavDof2/6/15 and PavARF8 containing BglII/SpeI restriction sites were amplified and cloned into the pCAMBIA1302 vector, which enabled PavDof2/6/15 and PavARF8 to be fused with GFP. Transformation was carried out as previously described (Liu et al., 2010 (link)). Fluorescence signals were observed and captured using an Olympus FluoView 3000 confocal microscope equipped with Olympus FluoView FV10-ASW 4.0 Viewer Software. GFP was excited at 488 nm and the emitted signal was captured at 500–540 nm. Images were captured using 5% of the maximum light intensity value and gain of 600–650.

The full-length coding sequences of VvMYB20, VvMYB58, VvMYB100, VvMYB170, VvMYB191, VvMYB205, VvMYB251, VvMYB258, and VvMYB263 without a stop codon were amplified from the cDNA obtained from “Crimson seedless” grapevine berries using gene-specific primers. The fragments were identified by sequencing and fused to the green fluorescent protein (GFP) under the control of the double CaMV35S promoter in the modified plant expression vector pCam35s-GFP to produce pVvMYB20-GFP, pVvMYB58-GFP, pVvMYB100-GFP, pVvMYB170-GFP, pVvMYB191-GFP, pVvMYB205-GFP, pVvMYB251-GFP, pVvMYB258-GFP, and pVvMYB263-GFP plasmids, respectively. All recombinant plasmids were independently transferred into the agrobacterium strain GV3101 and infiltrated in N. benthamiana leaves as described in a previous work [35 (link)]. Fluorescence signals in the cells were visualized 72 h post-agroinfiltration using an Olympus FluoView 3000 confocal microscope equipped with an Olympus FluoView FV10-ASW 4.0 Viewer Software. Fluorescence images were captured at an excitation wavelength of 488 nm.