Aav1 syn flex jgcamp7f wpre

Manufactured by Addgene

AAV1-syn-FLEX-jGCaMP7f-WPRE is a viral vector construct containing a Cre-dependent GCaMP7f calcium indicator under the control of the synapsin promoter. This vector is designed for use in adeno-associated virus (AAV) production and delivery to enable Cre-dependent expression of the calcium indicator protein in target cells.

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Lab products found in correlation

Aav1 hsyn1 sio stgtacr2 fusionred, by Addgene (2 mentions) Aav1 syn jgcamp7f wpre, by Addgene (1 mentions) Aav5 hsyn dio hm4d gi mcherry, by Addgene (1 mentions) Aav5 ef1a dio enphr 3.0 eyfp, by Addgene (1 mentions)

2 protocols using aav1 syn flex jgcamp7f wpre

Adeno-Associated Virus Vector Protocol

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AAV1-hSyn1-SIO-stGtACR2-FusionRed (Catalog # 105677-AAV1), AAV1-syn-jGCaMP7f-WPRE (Catalog # 104488-AAV1), AAV1-syn-FLEX-jGCaMP7f-WPRE (Catalog # 104492-AAV1), AAVretro-hSyn-Con/Fon-hChR2-EYFP (Catalog # 55645-AAVrg), AAV5-EF1α-Con/Fon-GCaMP6f (Catalog # 137122-AAV5), and AAVretro-hSyn-HI.EGFP-Cre-WPRE (Catalog # 105540-AAVrg) were purchased from Addgene. AAV5-EF1α-DIO-GCaMP6s, AAV2-EF1α-DIO-hChR2(H134R)-EYFP, AAV5-hSyn-Con/Fon-hChR2(H134R)-EYFP, AAV5-hSyn-Con/Foff-hChR2-EYFP, AAV5-hSyn-Coff/Fon-hChR2(H134R)-EYFP, AAV2-EF1α-FLEX-mCherry, AAV2-EF1α-DIO-EYFP, AAV5-hSyn-Con/Fon-EYFP, AAV5-hSyn-Con/Foff-EYFP, AAV5-hSyn-Coff/Fon-EYFP were purchased from the University of North Carolina vector core. AAV2-EF1α-FLEX-ChR2-nuclear hrGFP was purchased from the University of Pennsylvania vector core.

Wu Y.E., Dang J., Kingsbury L., Zhang M., Sun F., Hu R.K, & Hong W. (2021). Neural control of affiliative touch in prosocial interaction. Nature, 599(7884), 262-267.

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Optogenetic and chemogenetic tools for neural modulation

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The following AAVs along with the supplier, injection titres in viral genome copies/ml (vg/ml) and injection volumes in nanoliters (nl) were used in this study.
AAV1-hSyn1-SIO-stGtACR2-FusionRed (Addgene 105677, ~2 × 10¹² vg/ml, 200nl), AAV5-hSyn-DIO-hM4D(Gi)-mCherry (Addgene 44362, ~2 × 10¹² vg/ml, 200nl per injection), AAV5-Ef1a-DIO-eNpHR3.0-eYFP (Halo-eYFP, E.D. Fig. 2) (Addgene 26966, ~4 × 10¹² vg/ml, 200nl per injection), AAV1-syn-FLEX-jGCaMP7f-WPRE (Addgene 104492, ~2 × 10¹² vg/ml, 200nl per injection), AAVdj-syn-FLEX-FlpO (Janelia Vector Core, ~1 × 10¹³ vg/ml, 200nl per injection), AAVretro-syn-fDIO-jGCaMP7f (Janelia Vector Core, ~2 × 10¹² vg/ml, 200nl per injection), AAVretro-CBH-DIO-nls-mScarlet (Janelia Vector Core, ~1 × 10¹³ vg/ml, 200nl per injection), AAVretro-CBH-DIO-nls-mNeonGreen (Janelia Vector Core, ~1 × 10¹³ vg/ml, 200nl per injection).

Yang B., Karigo T, & Anderson D.J. (2022). TRANSFORMATIONS OF NEURAL REPRESENTATIONS IN A SOCIAL BEHAVIOR NETWORK. Nature, 608(7924), 741-749.

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