Sd oct system

Manufactured by Bioptigen

Sourced in United States

The SD-OCT system is a high-resolution optical imaging device that uses low-coherence interferometry to capture cross-sectional images of biological samples. It provides detailed structural information about the sample by analyzing the interference patterns of light reflected from different depths within the tissue.

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Lab products found in correlation

Systane, by Alcon (2 mentions) Matlab, by MathWorks (1 mentions) Tropicamide ophthalmic solution 1, by Bausch & Lomb (1 mentions) Micron 2 retinal imaging microscope, by Phoenix Pharmaceuticals (1 mentions) Genteal, by Novartis (1 mentions) Phenylephrine, by Akorn (1 mentions) Phenylephrine hydrochloride eye drops, by Akorn (1 mentions)

Spelling variants of this product

SD-OCT (37 citations) OCT) system (6 citations) SD-OCT Bioptigen® Spectral Domain Ophthalmic Imaging System (5 citations) 840HHP SD-OCT system (3 citations) SD-OCT system with 1180 nm center wavelength broadband light source (3 citations) SD-OCT system with 820 nm center wavelength broadband light source (2 citations) Envisu R2200 SD-OCT ophthalmic imaging system (2 citations)

20 protocols using sd oct system

Measuring CNV Size with OCT Imaging

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CNV size was determined using optical coherent tomography (OCT) [84 (link)]. OCT was performed using an SD-OCT system (Bioptigen Inc., Durham, NC), with scan parameters set to 1.6 × 1.6 mm rectangular volume scans, consisting of 100 B-scans (1000 A-scans per B scan). Mice were anesthetized and pupils were dilated as described above. Using the Bioptigen SD-OCT system, the center of the lesion was determined by identifying the midline of the RPE/Bruch’s membrane rupture [84 (link)], and Image J software (http://imagej.nih.gov/ij/) was used to measure the cross-sectional area of the hyporeflective spot seen in the fundus image (en face) as well as the area of fluid accumulation in the outer retina (cross-sectional view).

Obert E., Strauss R., Brandon C., Grek C., Ghatnekar G., Gourdie R, & Rohrer B. (2017). Targeting The Tight Junction Protein, Zonula Occludens-1, With The Connexin 43 Mimetic Peptide, αCT1, Reduces VEGF-Dependent RPE Pathophysiology. Journal of molecular medicine (Berlin, Germany), 95(5), 535-552.

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Non-invasive Retinal Imaging in Mice

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Mouse retinas were imaged non-invasively using a spectral domain optical coherence tomography (SD-OCT) system (Bioptigen, Research Triangle Park, NC) that was optimized for small animals. The mice were anaesthetized, wrapped in a blanket, and placed on the OCT imaging stage.^{27 (link)} The eyes were dilated with 10% topical phenylephrine eye drops and kept moist using artificial tears (Systane, Alcon, TX). Imaging scans covered a volume of 1.3 × 1.3 × 1.56 mm³ of the mouse eye centered on the optic disk and 100 b-scans were generated, and b-scan density was 1000 a-scans/b-scan. Photoreceptor layer thickness was quantified using MATLAB (Mathworks, Natick, MA) and segmentation software and applications developed by the Ophthalmic Biophysics Center at the Bascom Palmer Eye Institute, University of Miami.^{27 (link)} The thickness of the outer nuclear layer and inner and outer segments of the photoreceptors were measured in 70–80 cross-sectional b-scans and then averaged across the retina.^{23 (link), 28 (link)}

Patel A.K., Akinsoji E, & Hackam A.S. (2015). Defining the relationships among retinal function, layer thickness and in visual behavior during oxidative stress-induced retinal degeneration. Current eye research, 41(7), 977-986.

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Spectral-Domain OCT Retinal Thickness Measurement

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A spectral-domain OCT (SD-OCT) system (Bioptigen, Morrisville, NC, USA) was used to measure retinal thickness in each mouse model. Tropicamide (0.5%) drops were used to stimulate eye dilation. The mice were anesthetized with ketamine and xylazine (50 mg/kg and 5 mg/kg bodyweight, respectively). One rectangular scan consisting of 1000 A-scans by 100 B-scans over a 1.4 × 1.4-mm area centered on the optic nerve head (ONH) was done on each eye of each animal. The scans were processed using the Bioptigen Diver analysis software. Retinal thickness was measured at 0.35 mm from the ONH. The inner, outer, and total thicknesses of four points (nasal, temporal, superior, and inferior) on the retina were averaged across the points.

Sheskey S.R., Antonetti D.A., Rentería R.C, & Lin C.M. (2021). Correlation of Retinal Structure and Visual Function Assessments in Mouse Diabetes Models. Investigative Ophthalmology & Visual Science, 62(10), 20.

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Optical Coherence Tomography of Rat Eyes

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Rats were anesthetized and pupils were dilated as previously described.^{19 (link)} Optical coherence tomography (OCT) was performed with an SD-OCT system (Bioptigen, Inc., Durham, NC, USA). Volume analyses were performed using 100 horizontal raster and consecutive B scan lines composed of 1200 A scans. The volume size was 1.6 × 1.6 mm. Both eyes of each rat were examined before treatment and at 2 weeks and 1 month post NaIO3 injection.

Xiao J., Yao J., Jia L., Lin C, & Zacks D.N. (2017). Protective Effect of Met12, a Small Peptide Inhibitor of Fas, on the Retinal Pigment Epithelium and Photoreceptor After Sodium Iodate Injury. Investigative Ophthalmology & Visual Science, 58(3), 1801-1810.

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Retinal Thickness Measurement via SD-OCT

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After ERG recordings, the animals were transferred to the recording plane of the SD-OCT system (Bioptigen, Durham, NC) when they were anesthetized. Their pupils were dilated with 1% atropine and 2.5% phenylephrine hydrochloride (Xing Qi, Shenyang, People’s Republic of China). A corresponding box was centered on the optic nerve head (ONH) with eight measurement points separated by 3 mm from each other. The SD-OCT cross-sectional images were analyzed with the InVivoVueTM DIVER 2.4 software (Bioptigen, Inc, NC, USA). The neural retinal thickness for examined eyes was compared at each point by measuring the distance from the vitreous face of the RGCs layer to the apical face of the RPE layer. OCT images were acquired after noise reduction of the average.

Tao Y., Yang Z., Fang W., Ma Z., Huang Y.F, & Li Z. (2017). Adeno-associated virus-mediated neuroglobin overexpression ameliorates the N-methyl-N-nitrosourea-induced retinal impairments: a novel therapeutic strategy against photoreceptor degeneration. Therapeutics and Clinical Risk Management, 13, 1379-1389.

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Fundus and OCT Imaging in Anesthetized Rabbits

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Fundus photographs and OCT were taken using a spectral domain (SD)-OCT system (Bioptigen, Inc., Durham, NC, USA). Rabbits were deeply anesthetized with intramuscular anesthesia consisting of ketamine 30 to 50 mg/kg body weight, xylazine 5 to 10 mg/kg body weight, and acepromazine 1 mg/kg body weight. Depth of anesthesia was verified by the absence of the toe pinch withdrawal reflex. The pupils were dilated with topical 1% tropicamide to view the fundus.^{38 (link),39 (link)}

Ma G., Duan Y., Huang X., Qian C.X., Chee Y., Mukai S., Cui J., Samad A., Matsubara J.A., Kazlauskas A., D'Amore P.A., Gu S, & Lei H. (2016). Prevention of Proliferative Vitreoretinopathy by Suppression of Phosphatidylinositol 5-Phosphate 4-Kinases. Investigative Ophthalmology & Visual Science, 57(8), 3935-3943.

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High-Resolution 3D Retinal Imaging

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Ultra high resolution SD-OCT imaging was performed on both eyes from all groups, at baseline 1 week prior and 1 and 2-weeks post-injection using Bioptigen SD-OCT system (Research Triangle Park, NC). A series of 100 b-scans were collected, stacked and aligned spatially to form a registered three-dimensional rendering of retinal volume.

Sarfare S., Dacquay Y., Askari S., Nusinowitz S, & Hubschman J.P. (2015). Biocompatibility of a Synthetic Biopolymer for the Treatment of Rhegmatogenous Retinal Detachment. Journal of clinical & experimental ophthalmology, 6(5), 475.

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In vivo Optical Coherence Tomography of Mouse Eyes

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Optical coherence tomography (OCT) is a noninvasive procedure that allows visualization of internal microstructure of various eye structures in living animals. An SD-OCT system with 820 nm center wavelength broadband light source (Bioptigen, NC) was used for in vivo non-contact imaging of eyes from control or EAU mice. Mice were anesthetized and the pupils dilated as described above. Mice were then immobilized using adjustable holder that could be rotated easily allowing for horizontal or vertical scan scanning. Each scan was performed at least twice, with realignment each time. The dimension of the scan (in depth and transverse extent) was adjusted until the optimal signal intensity and contrast was achieved. Retinal thickness was measured from the central retinal area of all images obtained from both horizontal and vertical scans from the same eye, using the system software, and averaged. The method used to determine the retinal thicknesses in the system software was as described^{29 (link)}.

Oladipupo F.O., Yu C.R., Olumuyide E., Jittaysothorn Y., Choi J.K, & Egwuagu C.E. (2020). STAT3 deficiency in B cells exacerbates uveitis by promoting expansion of pathogenic lymphocytes and suppressing regulatory B cells (Bregs) and Tregs. Scientific Reports, 10, 16188.

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Corneal Thickness Imaging in Mice

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Prior to and following infection, the corneas of mice were imaged using a Bioptigen SD-OCT system (Durham, NC, USA) for corneal thickness. Images were acquired using Bioptigen SD-OCT software (InVivoVue Clinic, Bioptigen, Morrisville, NC, USA).

Berube A., Gmyrek G.B., Royer D.J, & Carr D.J. (2022). Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection. Viruses, 14(3), 589.

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Optical Coherence Tomography of Mouse Eyes

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The OCT procedure was described previously^{13 (link)}. In brief, mice were anaesthetised with an intraperitoneal injection of a mixture of ketamine (100 mg kg⁻¹) and xylazine (20 mg kg⁻¹) and were kept warm on a heating blanket. Pupils were dilated with a topically applied eye drop of 1% tropicamide. Radial volumetric images, centered on the optic nerve head, were acquired from either the left or right eye using the high resolution Bioptigen SD-OCT system. All of the analyses were carried out in a double masked fashion.

Yu D., Zheng J., Zhu R., Wu N., Guan A., Cho K.S., Chen D.F, & Luo G. (2015). Computer-aided analyses of mouse retinal OCT images – an actual application report. Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists), 35(4), 442-449.

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