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Single cell low input cdna synthesis amplification module

Manufactured by New England Biolabs
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The Single Cell/Low Input cDNA Synthesis & Amplification Module is a laboratory tool designed for the synthesis and amplification of complementary DNA (cDNA) from small input samples, such as single cells or low-input RNA. The module enables the conversion of RNA into cDNA and subsequent amplification, providing a solution for applications that require analysis of genetic material from limited sample sizes.

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NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (12 citations) NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (7 citations) NEB Single Cell/Low Input cDNA Synthesis and Amplification Module (3 citations)

2 protocols using single cell low input cdna synthesis amplification module

1

Single-Nuclei RNA Extraction and Amplification

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted from the single-nuclei suspension containing 300,000 nuclei by using the RNeasy Mini Kit (74104), which involved on-column gDNA digestion before RNA elution. cDNA was synthesized and amplified with NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (E6421S) by following the manufacturer’s protocol.
Hardwick S.A., Hu W., Joglekar A., Fan L., Collier P.G., Foord C., Balacco J., Lanjewar S., Sampson M.M., Koopmans F., Prjibelski A.D., Mikheenko A., Belchikov N., Jarroux J., Lucas A.B., Palkovits M., Luo W., Milner T.A., Ndhlovu L.C., Smit A.B., Trojanowski J.Q., Lee V.M., Fedrigo O., Sloan S.A., Tombácz D., Ross M.E., Jarvis E., Boldogkői Z., Gan L, & Tilgner H.U. (2022). Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue. Nature Biotechnology, 40(7), 1082-1092.
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2

Single-Nuclei RNA Extraction and Amplification

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted from the single-nuclei suspension containing 300,000 nuclei by using RNeasy Mini Kit (Catalog No. 74104), which involved on-column gDNA digestion before RNA elution. cDNA was synthesized and amplified with NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (Catalog no. E6421S) by following the manufacturer’s protocol.
Hardwick S.A., Hu W., Joglekar A., Fan L., Collier P.G., Foord C., Balacco J., Lanjewar S., Sampson M.M., Koopmans F., Prjibelski A.D., Mikheenko A., Belchikov N., Jarroux J., Lucas A.B., Palkovits M., Luo W., Milner T.A., Ndhlovu L.C., Smit A.B., Trojanowski J.Q., Lee V.M., Fedrigo O., Sloan S.A., Tombácz D., Ross M.E., Jarvis E., Boldogkői Z., Gan L, & Tilgner H.U. (2022). Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue. Nature Biotechnology, 40(7), 1082-1092.
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