Pd150606 d5946

Manufactured by Merck Group

Sourced in United States

PD150606 (D5946) is a laboratory instrument produced by Merck Group. The device is used for scientific research and analysis applications. The core function of this product is to perform measurements and data collection. Further details about its intended use or capabilities are not provided in this factual and unbiased description.

Automatically generated - may contain errors

Lab products found in correlation

Ab52757, by Abcam (1 mentions) Ab134175, by Abcam (1 mentions) Mettl3, by ABclonal (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Sw480, by American Type Culture Collection (1 mentions) Sw620, by American Type Culture Collection (1 mentions) Dld 1, by American Type Culture Collection (1 mentions) Cyclin d1, by Abcam (1 mentions) Cisplatin, by Selleck Chemicals (1 mentions) Mln4924 b1036, by Apexbio (1 mentions)

2 protocols using pd150606 d5946

Colorectal Cancer Cell Line Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human CRC cell lines SW480, SW620, DLD1, RKO were obtained from the American Type Culture Collection (ATCC). All cells were routinely cultured in RPMI 1640 (Invitrogen; 11875–093) or DMEM (Invitrogen; 11965–092) supplemented with 10% fetal bovine serum. All cells were incubated at 37 °C with 5% CO2 and 95% humidity. The following antibodies were used for western blotting and IHC: HSF1 (12972S, Cell Signaling Technology, CST; ab52757, Abcam); β-catenin (8480S, CST); β-actin (4970 L, CST); FLAG (F1804–1, Sigma); cyclinD1 (ab134175, Abcam); Cleaved PARP1 (9541-s, CST); METTL3 (a8370, Abclonal); GLS1 (ap8809b, Abgent). Pyrvinium (P0027), LiCl (793620), cycloheximide (R750107), chloroquine (C6628), MG132 (474790) and PD150606 (D5946) were purchased from Sigma-Aldrich.

Song P., Feng L., Li J., Dai D., Zhu L., Wang C., Li J., Li L., Zhou Q., Shi R., Wang X, & Jin H. (2020). β-catenin represses miR455-3p to stimulate m6A modification of HSF1 mRNA and promote its translation in colorectal cancer. Molecular Cancer, 19, 129.

+ Open protocol

+ Expand

Drug Resistance Mechanism Research

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Drug resistant cells SGC-R and BGC-R cells were developed from SGC7901 and BGC823, respectively 16 (link). All cell lines were cultured as previously reported. The chemical reagents including Dichloroacetic acid (DCA) (D54702), KNK437 (SML0964), Cycloheximide (R750107), chloroquine (C6628), MG132 (474790) and PD150606 (D5946) were purchased from Sigma Aldrich (USA). MLN4924 (B1036) and radicicol (A4067) were purchased from ApexBio (USA). Cisplatin and KRIBB11 (S840201) were obtained from Selleck (Shanghai, China).

Xu J., Shi Q., Xu W., Zhou Q., Shi R., Ma Y., Chen D., Zhu L., Feng L., Cheng A.S., Morrison H., Wang X, & Jin H. (2019). Metabolic enzyme PDK3 forms a positive feedback loop with transcription factor HSF1 to drive chemoresistance. Theranostics, 9(10), 2999-3013.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!