Lipozyme calb

Lipase B from Candida antarctica (Lipozyme® CALB), Thermomyces lanuginosus lipase (Lipozyme® TL 100L, TLL), catalase from Aspergillus niger (Catazyme® 25L, CAT), and β-galactosidase from Kluyveromyces lactis (Lactozyme® pure 6500L, LAC) solutions were from Novozymes (Denmark). 4-Methoxybenzenediazonium tetrafluoroborate (MBDFB), iodobenzene, palladium acetate, sodium tetrachloropalladate (Na₂[PdCl₄]), and CuSO₄·5H₂O were from Sigma-Aldrich (now Merk). N-Acetyl-l-tryptophan methyl ester (TrpOMe) was from Alfa Aesar. Methanol and acetonitrile of HPLC grade were purchased from Scharlab (Scharlab, S.L., Barcelona, Spain). Cu-FOS2 and Cu-NR hybrids were synthesized following the previously reported strategy.^{18 (link)}

5-Hydroxymetyl-2-furancarboxylic acid (HMFA, >97%), was acquired from Carbosynth Compton, Berkshire, UK). ε-Caprolactone (ECL, ≥98%), tetrahydrofuran (THF, >99%), toluene (>99%), chloroform (CHCl₃, >99%), dichloromethane (CH₂Cl₂, ≥99,8%), acetonitrile (>99%), sodium sulphate (Na₂SO₄, >99%) were from Sigma Aldrich (Steinheim am Albuch, Germany). Magnesium chloride (MgCl₂, 98%) and potassium carbonate (K₂CO₃, 99%) were purchased from Chimopar S.A. (Bucharest, Romania), while sodium chloride (NaCl, >99%) and potassium sulfate (K₂SO₄, 99%) were products of VWR Chemicals (Leuven, Belgium) and Alfa Aesar GmbH & Co KG (Karlsruhe, Germany), respectively. All chemicals were used as purchased, without further purification.
Three commercially available immobilized enzymes were utilized as biocatalysts for the polymerization reactions: Novozyme 435 (lipase from Candida antarctica B immobilized on acrylic resin) and Lipozyme CalB, (from C. antarctica) were products of Novozymes (Bagsværd, Denmark), while GF-CalB-IM (lipase B from C. antarctica immobilized on microporous ion exchange resin) was purchased from GenoFocus (Daejeon, South Korea). Lipase from porcine pancreas (Sigma-Aldrich, Steinheim, Germany) was used for the enzymatic degradation studies.

Lipase from Candida rugosa Type VII, ≥700 U/mg (CRL) was purchased from Merck Life Science S.L.U. (Madrid, Spain). Lipase B from Candida antarctica (Lipozyme^®, CAL-B), lipase A from Candida antarctica (NovoCor^®AD L, CAL-A), lipase from Rhizomucor miehei (Palatase 20000; RML), lipase from Thermomyces lanuginosus (TLL) and Lecitase^®ultra (LU, chimera produced through the fusion of the genes of the lipase from Thermomyces lanuginosus and the phospholipase A1 from Fusarium oxysporum) were kindly gifted from Novozymes Spain.