The dorsal quadrants of the L4-L5 spinal dorsal horn were rapidly harvested and homogenized in PBS followed by centrifugation at 4°C for 15 min at 14,000 × g. The supernatants were collected to detect the concentrations of TNF-α, IL-1β, IL-6, and IL-10 using corresponding ELISA kits (cat no. EK0526, EK0393, EK0412, EK0418; Boster Biological Technology). According to the manufacturer’s instructions, the absorbance was detected at 450 nm (A450) and a standard curve was delineated based on the absorbance of standards.
Ek0412
Manufactured by Boster Bio
Sourced in China
The EK0412 is a laboratory equipment product. It is designed for conducting various experiments and analyses in a research or testing environment. The core function of the EK0412 is to provide a controlled and standardized platform for performing specific tasks or procedures. The detailed specifications and intended use of this product are not available in this response.
Automatically generated - may contain errors
Lab products found in correlation
Ek0393, by Boster Bio (2 mentions)
Ek0526, by Boster Bio (2 mentions)
Ke20001, by Proteintech (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Ek0418, by Boster Bio (1 mentions)
Tnf α, by Boster Bio (1 mentions)
Skanit software, by Thermo Fisher Scientific (1 mentions)
S0131, by Beyotime (1 mentions)
S0103, by Beyotime (1 mentions)
A061 1, by Nanjing Jiancheng (1 mentions)
Multiscan fc plate reader, by Thermo Fisher Scientific (1 mentions)
A095 1 1, by Nanjing Jiancheng (1 mentions)
6 protocols using ek0412
1
Quantifying Spinal Inflammatory Mediators
2
Rat MCAO Stroke Model with NBP
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The nylon monofilaments (a2432A2) used for inducing middle cerebral artery occlusion (MCAO) stroke in a rat model were obtained from Beijing Cinontech Co., Ltd. Butylphthalide (NBP) soft capsules was obtained from CSPC Pharmaceutical Group Co., Ltd. Neuron-specific nuclear protein (NeuN) antibody (BM4354), ionized calcium binding adaptor molecule 1 (Iba-1) antibody (PB0517), and interleukin-1β (IL-11β), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) enzyme-linked immunosorbent assay (ELISA) kits (EK0393, EK0412, EK0526) were purchased from Boster Biological Technology Co., Ltd. Sodium pentobarbital (P3761) was obtained from Merck KGaA (Darmstadt, Germany).
3
Oxidative Stress and Inflammatory Response Quantification
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Detection of the SOD activity, content of ATP, GSH, GSSG, and MDA was quantified by thiobarbituric acid assay, colorimetric method, Microplate method and WST-8 according to the manufacturer's instructions (S0131 and S0103, Beyotime Biotechnology, Haimen, China; A095-1-1 and A061-1, Nanjing Jiancheng Bioengineering Institute, China). Results were obtained using a Multiscan FC plate reader with SkanIt software (Thermo Scientific).
Inflammatory cytokine (TNF-α and IL-6) levels in the liver were measured by ELISA according to the manufacturer's protocols (KE20001, Proteintech, Wuhan, China; EK0412, Boster Biological Technology, Wuhan, China).
Inflammatory cytokine (TNF-α and IL-6) levels in the liver were measured by ELISA according to the manufacturer's protocols (KE20001, Proteintech, Wuhan, China; EK0412, Boster Biological Technology, Wuhan, China).
4
Sandwich ELISA for IL-6 Quantification
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The protocol followed was provided from the manufacturer standard sandwich enzyme-linked immunosorbent assay kit (EK0412, Boster Biological Technology Ltd, USA). The lowest limit of IL-6 detection levels was under 62.5–4000 pg/mL (The optical densities were read at 450 nm).
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The protocol followed was provided from the manufacturer standard sandwich enzyme-linked immunosorbent assay kit (EK0412, Boster Biological Technology Ltd, USA). The lowest limit of IL-6 detection levels was under 62.5–4000 pg/mL (The optical densities were read at 450 nm).
5
Liver Cytokine Quantification Protocol
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6
Quantifying Hepcidin Levels in Serum
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According to the manufacturer's instructions, the levels of hepcidin in the serum were detected using an enzyme-linked immunosorbent assay kit (#EK0412, Boster Biological Technology, China). Briefly, 50 μl enzyme-labeled reagents were added to the 96-well-plate, except the blank control. After mixing, the orifice plates were incubated at 37°C for 30 min, followed by the addition of reaction substrates A and B (50 μl each time), and then dark incubation at 37°C for 10 min. The absorbance value of 450 nm wavelength was measured by a microplate reader (ThermoFisher, USA) after adding quenching solution for 15 min. The regression method is used to study the optical density (OD) value to deduce the sample concentration further.
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