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Sqrt pcr rnaiso plus

Manufactured by Takara Bio
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Sourced in China

SqRT-PCR RNAiso Plus is a reagent for the isolation of high-quality total RNA from a variety of biological samples. It is based on the guanidinium thiocyanate-phenol-chloroform extraction method and provides efficient RNA purification with minimized DNA and protein contamination.

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2 protocols using sqrt pcr rnaiso plus

1

Gill Tissue RNA Extraction and qRT-PCR

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Total RNA was isolated from gill tissue using sqRT-PCR RNAiso Plus (TaKaRa, Dalian, China). The cDNA was synthesized using the Perfect Real Time version of the PrimerScriptTM RT reagent kit with gDNA Eraser (Perfect Real Time) (TaKaRa,) according to manufacturer’s instructions. Then, sq-RT-PCR were chosen to analyze genes, and performed in a total reaction volume of 25 μl according to the manufacturers’ instructions. The S. paramamosain beta-actin gene and 18S ribosomal RNA gene were selected as the internal control. Primers used in this study are listed in Additional file 1: Table S1.
Wang H., Tang L., Wei H., Lu J., Mu C, & Wang C. (2018). Transcriptomic analysis of adaptive mechanisms in response to sudden salinity drop in the mud crab, Scylla paramamosain. BMC Genomics, 19, 421.
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2

Transcriptional Analysis of Bombyx mori Silk Gland

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Total RNA was isolated from the BmPSG at the wandering stage using sqRT-PCR RNAiso Plus (TaKaRa, Dalian, China). The cDNA was synthesized using the Perfect Real Time version of the PrimerScript™ RT reagent kit with gDNA Eraser (Perfect Real Time) (TaKaRa, Dalian, China) according to the manufacturer’s instructions. Then, sqRT-PCR and qRT-PCR were selected to analyze the genes. Using ABI Stepone Plus (Ambion, Foster City, CA, USA) and the fluorescent dye SYBR Premix Ex Taq (TaKaRa), qRT-PCR was performed in a total reaction volume of 20 μl, according to the manufacturers’ instructions. The Bombyx NRp49 gene was selected as the internal control. Primers used in this study are listed in Supplementary Table S2 online.
Wang H., Wang L., Wang Y., Tao H., Yin W., SiMa Y., Wang Y, & Xu S. (2015). High yield exogenous protein HPL production in the Bombyx mori silk gland provides novel insight into recombinant expression systems. Scientific Reports, 5, 13839.
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