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K58 35

Manufactured by Merck Group
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Sourced in United States

The K58/35 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use. The core function of the K58/35 is to provide a controlled and stable environment for various laboratory experiments and processes.

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Lab products found in correlation

Gapdh 71, by Merck Group (2 mentions) Donkey anti rabbit hrp, by Jackson ImmunoResearch (1 mentions) Ab53494, by Abcam (1 mentions) Irdye 800cw, by LI COR (1 mentions) Ab8135, by Abcam (1 mentions) Alexa fluor 488 donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) S0819, by Merck Group (1 mentions) Alexa fluor 594 donkey anti mouse, by Jackson ImmunoResearch (1 mentions)

2 protocols using k58 35

1

Sodium Channel Protein Detection

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Primary antibodies used were mouse monoclonal antivoltage-gated sodium channel (1:5000; K58/35, Sigma Aldrich), mouse monoclonal anti-GAPDH (1:10000; GAPDH-71.1, Sigma Aldrich), and rabbit polyclonal anti-Biotin (1:10000; ab53494, Abcam, Cambridge, MA, USA). Secondary antibodies used were goat anti-mouse IRDye 800CW (1:10000; LI-COR, Biosciences), donkey anti-Rabbit-HRP (1:20000; Jackson ImmunoResearch, West Grove, PA, USA) for western blotting, and goat anti-mouse Cy3-conjugated (1:400; Jackson ImmunoResearch) for immunocytochemistry.
Gando I., Morganstein J., Jana K., McDonald T.V., Tang Y, & Coetzee W.A. (2017). Infant sudden death: Mutations responsible for impaired Nav1.5 channel trafficking and function. Pacing and clinical electrophysiology : PACE, 40(6), 703-712.
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2

Voltage-Gated Sodium Channel Immunodetection

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Primary antibodies used were mouse monoclonal anti-voltage-gated sodium channel (1:2000; K58/35, Sigma Aldrich, St. Louis, MO), mouse monoclonal anti-GAPDH (1:10000; GAPDH-71.1, Sigma), rabbit polyclonal anti-Nav1.5 (1:200; S0819, Sigma), rabbit polyclonal anti-Neurofilament heavy polypeptide (1:100; ab8135, Abcam, Cambridge, MA), mouse monoclonal anti-Nav1.8 (1:50; Neuromab clone N143/12), AlexaFluor 488 donkey anti-rabbit (1:200; Jackson ImmunoResearch, West Grave, PA), AlexaFluor 594 donkey anti-mouse (1:200; Jackson ImmunoResearch) and goat anti-mouse-HRP secondary (1:40000; Jackson ImmunoResearch)
Gando I., Williams N., Fishman G.I., Sampson B.A., Tang Y, & Coetzee W.A. (2019). Functional Characterization of SCN10A Variants in Several Cases of Sudden Unexplained Death. Forensic science international, 301, 289-298.
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