Pneumoslide igm kit

Peripheral blood was centrifuged in a procoagulant tube at 3000 rpm for 15 min, and serum was separated for determination of pathogen-specific IgM. The immunofluorescence-based Pneumoslide IgM kit (Vircell, Spain) was used to detect specific IgM antibody against Legionella, Mycoplasma pneumoniae, Q fever Rickettsia, Chlamydia pneumoniae, Adenovirus, respiratory syncytial virus, influenza A virus, influenza B virus, and parainfluenza virus. The cytomegalovirus (CVM) IgM ELISA kit (BioAssay, USA) was used to detect the IgM antibody response to CMV.

Serum samples of patients were collected and tested for the IgM of respiratory tract pathogens with Pneumoslide IgM kit (Vircell, Spain), including human respiratory syncytial virus, influenza A virus (subtypes H1N1 and H3N2), influenza B virus, parainfluenza virus 1/2/3, metapneumovirus, common coronavirus, Epstein-Barr virus, cytomegalovirus, rhinovirus, adenovirus, and bocavirus, as well as Legionella pneumophila serum type I, Mycoplasma pneumonia, and Chlamydia pneumoniae. Nasopharyngeal secretions were tested for nucleic acids of 13 respiratory pathogens (Health Gene Technologies, Ningbo, China). Sputum culture was performed to identify bacterial and fungal co-infection. The fungal examination was performed with Fungus (1-3)-β-D-Glucan kit (Dynamiker Biotechnology, Tianjin, China) and Platelia aspergillus Ag kit (Bio-rad, USA).