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2 protocols using sulfuric acid 98

1

Polyethylene Fiber Preparation Protocol

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Polyethylene fibers were prepared using HDPE pellets (LG Chem., LOTTE Chem., Korea) and LLDPE pellets (LOTTE Chem., Korea). Table S2 lists the specifications of the HDPE pellets and LLDPE pellets. Sulfuric acid 98% (Sigma Aldrich, USA) was used as a cross-linking agent.
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2

Osteogenic Differentiation of Stem Cells

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All chemical reagents including sulfuric acid 98%, NaNO3, KMnO4, H2O2 30% v/v, boric acid, glutaraldehyde, paraformaldehyde, 4′, 6-diamidino-2-phenylindole stain (DAPI), 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT reagent), dimethyl sulfoxide (DMSO), triton X-100, Alizarin Red S (ARS) dye, Graphite powder 20 μm synthetic, and PVA powder with 85 000-124 000 molecular weight and 99+% hydrolyzed were purchased from Sigma-Aldrich, Germany. For in vitro cell study, reagents including phosphate-buffered saline (PBS), collagenase type I, DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12F), fetal bovine serum (FBS), antibiotics (penicillin, streptomycin), and trypsin/ethylenediaminetetraacetic were purchased from Gibco, Germany. The osteogenic differentiation medium containing DMEM-F12, 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, 20 mM β-glycerol phosphate, 100 nM dexamethasone, 50 μM ascorbic acid was purchased from Bioidea, Iran.
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