For LC/MS sample preparation, LC/MS-grade water and acetonitrile (ACN) were used, if not otherwise stated, and were purchased together with acetone from Merck, Darmstadt, Germany. Triethylammonium bicarbonate buffer (TEAB), formic acid (FA), EDTA tris(2 carboxyethyl)phosphine (TCEP) and iodoacetamide (IAA) and trifluoroethanol (TFE) were purchased from Sigma-Aldrich, Taufkirchen, Germany. SDS was purchased from Serva Electrophoresis GmbH, Heidelberg, Germany. TFA was obtained from Roth, Karlsruhe, Germany. Rapigest was obtained from Waters, Milford, United States. 15N-labeled αSyn was purchased from rPeptide, Watkinsville, United States. MS-grade trypsin and LysN were obtained from Promega, Madison, United States. TMT10plex and TMT11-131C were purchased from Thermo Fisher Scientific, Bleiswijk, Netherlands.
Tmt11 131c
Manufactured by Thermo Fisher Scientific
Sourced in Netherlands
The TMT11-131C is a laboratory instrument designed for mass spectrometry analysis. It is used to perform quantitative proteomic studies by enabling the simultaneous detection and measurement of multiple protein samples. The device utilizes isobaric labeling technology to facilitate the comparative analysis of protein expression levels across different experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
Tmt10plex, by Thermo Fisher Scientific (3 mentions)
Lc ms grade water, by Merck Group (1 mentions)
Ms grade trypsin, by Promega (1 mentions)
Acetone, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Serva Electrophoresis (1 mentions)
Rapigest, by Waters Corporation (1 mentions)
Triethylammonium bicarbonate buffer, by Merck Group (1 mentions)
3 protocols using tmt11 131c
1
LC/MS Sample Preparation Protocols
2
Quantitative Proteome Profiling Using TMT
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Peptides were dissolved in water and TMT labels (Thermo Fisher TMT10plex, TMT11‐131C) (dissolved in acetonitrile) were added (with final acetonitrile concentration of 28.6%). Labeling reaction was conducted at RT for 1 h on a shaker. The reaction was quenched with 1.1% hydroxylamine for 15 min. Labeled samples were pooled and diluted with 0.05% formic acid to decrease acetonitrile concentration below 5%.
The labeling scheme for recovery assay with 11 TMT labels was as follows: mock‐shocked samples (mock shock, 1, 2, 3, and 5 h of recovery), pre‐shock control and heat‐shocked samples (heat shock, 1, 2, 3, and 5 h of recovery) given in the order of increasing TMT reporter ion mass (i.e., from 126 to 131C) (see Fig1 B).
The labeling scheme for 2D‐TPP with 10 TMT labels was as follows: mock shock replicate one (temperature one: TMT126, temperature two: TMT129N), mock shock replicate two (127N, 129C), heat shock replicate one (127C, 130N), heat shock replicate two (128N, 130C), and heat shock replicate three (128C, 131). In other words, samples from two adjacent temperatures of the temperature gradient were combined in each TMT set (see Fig5 A).
The labeling scheme for recovery assay with 11 TMT labels was as follows: mock‐shocked samples (mock shock, 1, 2, 3, and 5 h of recovery), pre‐shock control and heat‐shocked samples (heat shock, 1, 2, 3, and 5 h of recovery) given in the order of increasing TMT reporter ion mass (i.e., from 126 to 131C) (see Fig
The labeling scheme for 2D‐TPP with 10 TMT labels was as follows: mock shock replicate one (temperature one: TMT126, temperature two: TMT129N), mock shock replicate two (127N, 129C), heat shock replicate one (127C, 130N), heat shock replicate two (128N, 130C), and heat shock replicate three (128C, 131). In other words, samples from two adjacent temperatures of the temperature gradient were combined in each TMT set (see Fig
3
Quantitative Proteome Profiling by TMT Labeling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peptides were dissolved in water and TMT labels (ThermoFisher TMT10plex, TMT11-131C) (dissolved in acetonitrile) were added (with final acetonitrile concentration of 28.6%). Labeling reaction was conducted at RT for one hour on a shaker. The reaction was quenched with 1.1% hydroxylamine for 15 minutes. Labelled samples were pooled and diluted with 0.05% formic acid to decrease acetonitrile concentration below 5%.
The labelling scheme for recovery assay with eleven TMT labels was as follows: mock shocked samples (mock shock, one, two, three and five hours of recovery), pre-shock control and heat shocked samples (heat shock, one, two, three and five hours of recovery) given in the order of increasing TMT reporter ion mass (i.e., from 126 to 131C) (see Fig 1B).
The labelling scheme for 2D-TPP with ten TMT labels was as follows: mock shock replicate one (temperature one: TMT126, temperature two: TMT129N), mock shock replicate two (127N, 129C), heat shock replicate one (127C, 130N), heat shock replicate two (128N, 130C), heat shock replicate three (128C, 131). In other words, samples from two adjacent temperatures of the temperature gradient was combined in each TMT set (see Fig 5A).
The labelling scheme for recovery assay with eleven TMT labels was as follows: mock shocked samples (mock shock, one, two, three and five hours of recovery), pre-shock control and heat shocked samples (heat shock, one, two, three and five hours of recovery) given in the order of increasing TMT reporter ion mass (i.e., from 126 to 131C) (see Fig 1B).
The labelling scheme for 2D-TPP with ten TMT labels was as follows: mock shock replicate one (temperature one: TMT126, temperature two: TMT129N), mock shock replicate two (127N, 129C), heat shock replicate one (127C, 130N), heat shock replicate two (128N, 130C), heat shock replicate three (128C, 131). In other words, samples from two adjacent temperatures of the temperature gradient was combined in each TMT set (see Fig 5A).
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