Appropriate antibody igg isotype controls

Manufactured by BD

Appropriate antibody IgG isotype controls are laboratory reagents used to ensure the specificity of antibody-based experiments. These controls help researchers distinguish specific antigen-antibody interactions from non-specific binding. Isotype controls match the isotype of the primary antibody being used, providing a tool to assess background signal and validate experimental results.

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Lab products found in correlation

Facsaria 3, by BD (2 mentions) Facsdiva 8, by BD (1 mentions) Facsverse, by BD (1 mentions) Anti cd16 cd32 clone 2.4g2, by BD (1 mentions) Percoll, by GE Healthcare (1 mentions)

Close spelling variants of this product

Appropriate isotype control antibody Appropriate isotype controls Isotype control IgG antibody IgG isotype control Isotype control antibodies IgG control antibody Isotype controls Control IgG Appropriate antibody Isotype IgG

2 protocols using appropriate antibody igg isotype controls

Isolation and Analysis of Brain Immune Cells

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Mice were transcardially perfused with ice-cold PBS and brains were dissected. Single-cell suspensions were prepared and centrifuged over a 30%/70% discontinuous Percoll gradient (GE Healthcare), mononuclear cells were isolated from the interface, and total cell count determined. Isolated cells were labeled with fluorophore-conjugated monoclonal antibodies and sorted in a BD FACS Aria III. Flow cytometric analyses were performed on a FACS Verse or FACS Aria III using the FACSDiva 8.0 software (BD Biosciences) and data analyzed using the FlowJo v10.0.7 (Tree Star). Appropriate antibody IgG isotype controls (BD Biosciences) were used for all staining. Macrophages from the spleen were sorted after nonenzymatic disaggregation and were identified as LY6C⁺ F4/80^hi cells for comparison.

Arnold T.D., Lizama C.O., Cautivo K.M., Santander N., Lin L., Qiu H., Huang E.J., Liu C., Mukouyama Y.S., Reichardt L.F., Zovein A.C, & Sheppard D. (2019). Impaired αVβ8 and TGFβ signaling lead to microglial dysmaturation and neuromotor dysfunction. The Journal of Experimental Medicine, 216(4), 900-915.

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Spinal Cord Mononuclear Cell FACS Analysis

Cited 1 time

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For fluorescent-activated cell sorting (FACS) analysis, mice were transcardially perfused with 50ml 0.01 M PBS and their spinal cords were homogenized using a Teflon-stick homogenizer. Mononuclear cells were separated through Percoll (GE Healthcare Life Sciences) gradient centrifugation. Cells were pre-blocked with anti-CD16/CD32 (clone 2.4G2, BD Biosciences, 5 μg ml⁻¹), and stained on ice for 30 min with combinations of CD11b-PE-Cy^™7 (clone N418, eBioscience, 5 μg ml⁻¹) and FCRLS (clone 4G11, 5 μg ml⁻¹)^{29 (link)} conjugated to APC (Biolegend). Appropriate antibody IgG isotype controls (BD Biosciences) were used for all staining. FACS analysis was performed on a LSR machine (BD Biosciences), and data analyzed with FlowJo Software (TreeStar).

Butovsky O., Jedrychowski M.P., Cialic R., Krasemann S., Murugaiyan G., Fanek Z., Greco D.J., Wu P.M., Doykan C.E., Kiner O., Lawson R.J., Frosch M.P., Pochet N., Fatimy R.E., Krichevsky A.M., Gygi S.P., Lassman H., Berry J., Cudkowicz M.E, & Weiner H.L. (2014). Targeting miR-155 restores abnormal microglia and attenuates disease in SOD1 mice. Annals of neurology, 77(1), 75-99.

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