Namalwa cells

Jurkat (TIB-152), Namalwa cells (CRL-1432), Nalm6 (CRL-3273), and K562 cells (CCL-243) were purchased from the ATCC and cultured in RPMI-1640 media supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin/streptomycin (1%P/S, Biowest) at 37°C in a 5% CO₂ and 85%–90% humidified incubator. HEK-293T cells were cultured in high-glucose Dulbecco’s Modified Eagle’s Media (DMEM, Biowest) supplemented with 10% FBS (Invitrogen) at 10% CO₂ atmosphere. MiaPaCa2 (CRL-1420) were cultured in DMEM supplemented with 10% FBS and 1% P/S, and SK-MES-1 (HTB-58) cells were cultured with DMEM (Gibco) supplemented with GlutaMAX, 10% FBS, and 100 U/mL of P/S (Invitrogen). Cells were maintained in incubators at 37°C with 5% CO₂ atmosphere and 85%–90% relative humidity. Absence of Mycoplasma spp. in cultured cells was routinely tested by a PCR-based assay (Minerva).

Namalwa cells (ATCC) were cultured in 96-well plates in complete medium (Table S5) at 2 × 10⁴ cells/well without or with 1 µg/ml Rituximab (Chimeric human, IgG1κ; Roche) or 1 µg/ml DARA. After 4 d, cells were harvested and incubated with 7AAD (BD Biosciences) for 5 min to identify late apoptotic cells. Proliferation was determined via cell counting. The same experiment was performed without or with 1 µg/ml DARA on the following cell lines (all from ATCC): Raji, DG75, and two cell lines derived from Ramos (NIP-IgM and NIP-IgM CD38-KO). Data were acquired by flow cytometry.