Anti hif 1α nb100 449

Manufactured by Novus Biologicals

Sourced in United States

Anti-HIF-1α (NB100-449) is a monoclonal antibody that binds to the HIF-1α protein. HIF-1α is a subunit of the hypoxia-inducible factor-1 (HIF-1) transcription factor, which plays a central role in the cellular and physiological response to low oxygen levels.

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Lab products found in correlation

Anti phospho smad1 5 8, by Cell Signaling Technology (1 mentions) Recombinant human bmp6, by R&D Systems (1 mentions) Anti hif 2α nb100 122, by Novus Biologicals (1 mentions) Mg132, by Peptide Institute (1 mentions) Anti α smooth muscle actin clone 1a4, by Merck Group (1 mentions) Anti flag m2 f3165, by Merck Group (1 mentions) Basic fibroblast growth factor (bfgf), by R&D Systems (1 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Tgf β, by R&D Systems (1 mentions) Anti mouse cd16 cd32 mouse fc block, by BD (1 mentions)

Close spelling variants of this product

Anti-HIF-1α NB100-449 HIF-1α

2 protocols using anti hif 1α nb100 449

Antibody-Based Analysis of BMP and VEGF Signaling

Cited 1 time

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Recombinant human BMP-6, BMP-9, VEGF, bFGF and TGF-β were
purchased from R&D Systems (R&D Systems, Bio-Techne, Minneapolis,
MN, USA). Cycloheximide (CHX) was purchased from Sigma-Aldrich (Merck,
Darmstadt, Germany), and MG132 was from Peptide Institute (Osaka, Japan). The
following antibodies were used: anti-FLAG M2 (F3165; Sigma-Aldrich, Merck),
anti-DDDDK (PM020; MBL, Tokyo, Japan) which recognizes the FLAG epitope,
anti-Myc, anti-HA (HA124; Nacalai Tesque, Kyoto, Japan), anti-HA (#561; MBL),
anti-α-tubulin (AC-15; Sigma-Aldrich, Merck), anti-SMAD1 (913C1b; Bio
Matrix Research, Chiba, Japan) which recognizes both SMAD1 and SMAD5 for ChIP
assays (5 (link)), anti-phospho-SMAD1/5/8 (Cell
Signaling Technology, Danvers, MA, USA), anti-PECAM1 (clone 18; BD bioscience,
San Jose, CA, USA), anti-HIF-1α (NB100-449, Novus Biologicals,
Bio-Techne), anti-HIF-2α (NB100-122, Novus Biologicals, Bio-Techne),
anti-α-smooth muscle actin (clone 1A4; Sigma-Aldrich, Merck), and
anti-HDAC1 (clone 2E10, #05-100; Millipore, Merck). A rabbit polyclonal
anti-ATOH8 antibody was raised against residues 306-321 of human ATOH8,
CTRTLQAEGRAKKRKE.

Morikawa M., Mitani Y., Holmborn K., Kato T., Koinuma D., Maruyama J., Vasilaki E., Sawada H., Kobayashi M., Ozawa T., Morishita Y., Bessho Y., Maeda S., Ledin J., Aburatani H., Kageyama R., Maruyama K., Heldin C.H, & Miyazono K. (2019). The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension. Science signaling, 12(607), eaay4430.

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Synthesis and Analysis of TEPP-46 and DASA-58

Cited 1 time

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TEPP-46 and DASA-58 were synthesized in accordance with published methods (12 (link), 13 (link)) and were a kind gift from Craig Thomas, NIH, MD, USA. LPS used was E. coli, serotype EH100 (Alexis). Western blotting antibodies were anti-PKM2 D78A4 (4053) and anti-β-actin (4267) from Cell Signaling Technologies, anti-mB7-H1 (PD-L1, AF1019) from R&D Systems, and anti-HIF-1α (NB100-449) from Novus. Anti-mouse-CD274PE (MIH5) from eBioscience and anti-mouse CD16/CD32 (Mouse BD Fc Block) from BD Pharmingen™ were used for FLOW cytometry in vitro. SYBR primers were from Eurofins, UK. Dimethyl sulfoxide used as vehicle control for TEPP-46 in all relevant in vitro assays. Antibodies used for flow cytometry analysis of in vivo tissues are listed in Table S1 in Supplementary Material.

Palsson-McDermott E.M., Dyck L., Zasłona Z., Menon D., McGettrick A.F., Mills K.H, & O’Neill L.A. (2017). Pyruvate Kinase M2 Is Required for the Expression of the Immune Checkpoint PD-L1 in Immune Cells and Tumors. Frontiers in Immunology, 8, 1300.

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