RNA was isolated from tissue samples with the RNeasy Mini kit (Qiagen) following the manufacturer’s instructions. RNA was extracted using the NucleoSpin RNA XS Kit for RNA-seq (Takara Bio) or the ARCTURUS PicoPure RNA Isolation kit (Thermo Fisher Scientific). The High-Capacity RNA-to-cDNA kit (Applied Biosystems) was used for reverse transcription. TaqMan gene expression assays were used to quantify target genes using TaqMan Fast Universal PCR Master Mix (Applied Biosystems) and primers for Cxcl12 (Mm00445553_m1), Vcam1 (Mm01320970_m1), Kitl (Mm00442972_m1), Angpt1 (Mm00456503_m1), Il6 (Mm00446190_m1), Vegfa (Mm00437306_m1) and Vcan (Mm01283063_m1, all FAM–MGB, Thermo Fisher Scientific) as well as Gapdh (Mm99999915_g1, VIC–MGB, Thermo Fisher Scientific). Samples were run on a 7500 Real-Time PCR system (Applied Biosystems), and target gene expression was normalized to Gapdh expression.
Nucleospin rna xs kit for rna seq
Manufactured by Takara Bio
The NucleoSpin RNA XS Kit is a laboratory product designed for the purification of total RNA from small samples. It utilizes a silica membrane-based technology to efficiently capture and purify RNA molecules from various sources, including cells and tissues. The kit is suitable for a wide range of applications, including RNA-seq, without providing any interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
High capacity rna to cdna kit, by Thermo Fisher Scientific (2 mentions)
Fam mgb, by Thermo Fisher Scientific (2 mentions)
Vic mgb, by Thermo Fisher Scientific (2 mentions)
Taqman fast universal pcr master mix, by Thermo Fisher Scientific (2 mentions)
7500 real time pcr system, by Thermo Fisher Scientific (2 mentions)
Rneasy mini kit, by Qiagen (2 mentions)
2 protocols using nucleospin rna xs kit for rna seq
1
Gene Expression Analysis in Tissue Samples
2
Gene Expression Analysis in Tissue Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RNA was isolated from tissue samples with the RNeasy Mini kit (Qiagen) following the manufacturer’s instructions. RNA was extracted using the NucleoSpin RNA XS Kit for RNA-seq (Takara Bio) or the ARCTURUS PicoPure RNA Isolation kit (Thermo Fisher Scientific). The High-Capacity RNA-to-cDNA kit (Applied Biosystems) was used for reverse transcription. TaqMan gene expression assays were used to quantify target genes using TaqMan Fast Universal PCR Master Mix (Applied Biosystems) and primers for Cxcl12 (Mm00445553_m1), Vcam1 (Mm01320970_m1), Kitl (Mm00442972_m1), Angpt1 (Mm00456503_m1), Il6 (Mm00446190_m1), Vegfa (Mm00437306_m1) and Vcan (Mm01283063_m1, all FAM–MGB, Thermo Fisher Scientific) as well as Gapdh (Mm99999915_g1, VIC–MGB, Thermo Fisher Scientific). Samples were run on a 7500 Real-Time PCR system (Applied Biosystems), and target gene expression was normalized to Gapdh expression.
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