515 pump system

General Procedures: All ¹³C NMR spectra were recorded in CDCl₃ on a Bruker AM 400 NMR spectrometer operating at a ¹³C resonance frequency of 100.61 MHz and processed with 1.0 Hz line broadening (zgig Bruker pulse program). ¹H NMR spectrum of phormidolide was recorded with residual CHCl₃ as the internal standard (δ_C 77.0, δ_H 7.26) on a Varian Unity 500 MHz spectrometer. ¹H NMR spectrum and 2D NMR spectra of phormidolide acetonide derivatives were obtained on a Bruker 600 MHz spectrometer equipped with a 1.7 mm MicroCryoProbe. Semi-preparative HPLC was carried out using a Waters 515 pump system equipped with a Waters 996 PDA. High resolution electrospray ionization mass spectra (HRESIMS) were obtained using an Agilent 1290 Infinity system with an Agilent 6530 Accurate Mass Q-TOF LC/MS.

Optical rotations were measured on a JASCO P-2000 polarimeter. LC-MS/MS analysis was carried out using a ThermoFinnigan LCQ AdvantageMax mass detector equipped with an electrospray ionization (ESI) source. HRESIMS spectra were obtained using an Agilent 1290 Infinity system with an Agilent 6530 Accurate Mass Q-TOF LC/MS, and ¹H NMR spectra were obtained using a Varian Unity 500 MHz spectrometer. Semipreparative HPLC was carried out using a Waters 515 pump system equipped with a Waters 996 PDA.