Egm 2 growth media

Manufactured by Lonza

Sourced in Australia

EGM-2 is a growth media manufactured by Lonza. It is designed to support the in vitro culture of endothelial cells.

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Lab products found in correlation

Egm 2 bulletkit, by Lonza (2 mentions) Axio imager 2 microscope, by Zeiss (1 mentions) Dii ac ldl, by Biomedical Technologies (1 mentions) Hmvec, by Lonza (1 mentions) Huvecs, by Lonza (1 mentions)

Close spelling variants of this product

EGM-2 (912 citations) EGM-2 media (141 citations) EGM media (12 citations) Growth media (5 citations)

3 protocols using egm 2 growth media

Endothelial Progenitor Cells LDL Uptake

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EPCs (10³ cells/well) were seeded on 1% fibronectin coated 24-well plates in EGM-2 Bullet Kit with 5% FBS (EGM-2 growth media, Lonza, Victoria, Australia) and allowed to attach following overnight incubation. For LDL uptake assay, cultured medium was replaced with 10 μg/ml of acetylated low density lipoprotein (Ac-LDL), labeled with 1,1′-dioctadecyl– 3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate (DiI-Ac-LDL, Biomedical Technologies), then cells were incubated for additional 4 h. After the end of the incubation period, the solution was aspirated and fresh growth medium was added before capturing the images using fluorescence microscopy (Zeiss AxioImager.2 microscope).

Hakami N.Y., Ranjan A.K., Hardikar A.A., Dusting G.J, & Peshavariya H.M. (2017). Role of NADPH Oxidase-4 in Human Endothelial Progenitor Cells. Frontiers in Physiology, 8, 150.

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Isolation and Culture of ECFCs

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Blood samples were obtained from the Puget Sound Blood Bank as either outdated whole blood (approximately 500 ml per unit) or leukocyte reduction filters, which yielded between 4-7×10⁸ leukocytes after elution. Whole blood was mixed 1:2 with phosphate-buffered saline (PBS) containing 10,000 units per liter heparin and 0.02% EDTA. Cells from leukocyte reduction filters were eluted with 200ml PBS containing 10,000 units per liter heparin and 0.02% EDTA. ECFCs were then isolated and cultured as previously described.²³ Individual colonies of cells were clonally expanded. All experiments were performed on cells between passage 8 and 15.
Primary mature human dermal microvascular endothelial cells (HMVECs) were obtained from Lonza. Cells were maintained in EGM-2 growth media (Lonza) and experiments were performed on cells between passage 7 and 12.

DiMaio T.A., Wentz B.L, & Lagunoff M. (2015). Isolation and Characterization of Circulating Lymphatic Endothelial Colony Forming Cells. Experimental cell research, 340(1), 159-169.

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Endothelial and Fibroblast Cell Culture

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A human umbilical vein endothelial cells (HUVECs) were purchased from Lonza, Victoria, Australia, and human microvascular endothelial cells (HMECs) were kind gifts from Centre for Disease Control and Prevention, Atlanta, USA. Mouse lung endothelial cells (MLECs) were immortalized using SV‐40T antigen. All endothelial cells types were cultured in EGM‐2 Bullet Kit with 5% foetal bovine serum (FBS), known as EGM‐2 growth media (Lonza, Mount Waverley, VIC, Australia). Human dermal fibroblasts were cultured in DMEM with 10% FBS. Endothelial and fibroblast cells were cultured in standard cell culture conditions using a 5% CO₂ incubator at 37°C. All high quality chemicals were purchased from Sigma‐Aldrich, Castle Hill, NSW, Australia unless otherwise specified.

Hakami N.Y., Dusting G.J, & Peshavariya H.M. (2016). Trichostatin A, a histone deacetylase inhibitor suppresses NADPH Oxidase 4‐Derived Redox Signalling and Angiogenesis. Journal of Cellular and Molecular Medicine, 20(10), 1932-1944.

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